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Literature summary for 1.1.1.1 extracted from

  • Solanki, K.; Abdallah, W.; Banta, S.
    Engineering the cofactor specificity of an alcohol dehydrogenase via single mutations or insertions distal to the 2'-phosphate group of NADP(H) (2017), Protein Eng. Des. Sel., 30, 373-380 .
    View publication on PubMed
Search for more literature for 1.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
G211C mutant enzyme exhibits almost complete reversals in cofactor specificity Pyrococcus furiosus
G211InsA with NADP+, the mutant enzyme shows a 29-fold increase in kcat as compared to the wild-type enzyme Pyrococcus furiosus
G211InsC with NAD+, the mutant enzyme exhibits 6-fold decrease in kcat as compared to wild-type enzyme. Mutant enzyme exhibits almost complete reversals in cofactor specificity Pyrococcus furiosus
G211InsG with NAD+, the mutant enzyme exhibits 2.5-fold enhancement in kcat over the wild-type enzyme. Activity with NADP+ exceeds that of the wild type enzyme Pyrococcus furiosus
G211InsS with NAD+, the mutant enzyme exhibits 2.5-fold enhancement in kcat over the wild-type enzyme. With NADP+, the mutant enzyme shows a 29-fold increase in kcat as compared to the wild-type enzyme Pyrococcus furiosus
G211S with NAD+, the mutant enzyme exhibits 1.5-fold enhancement in kcat over the wild-type. Activity with NADP+ exceeds that of the wild type enzyme Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.097
-
 NAD+ pH 8.8, 45°C, mutant enzyme G211InsC Pyrococcus furiosus
0.1
-
 NAD+ pH 8.8, 45°C, mutant enzyme G211S Pyrococcus furiosus
0.12
-
 NAD+ pH 8.8, 45°C, mutant enzyme G211InsA Pyrococcus furiosus
0.23
-
 NAD+ pH 8.8, 45°C, wild-type enzyme Pyrococcus furiosus
0.29
-
 NAD+ pH 8.8, 45°C, mutant enzyme G211InsG Pyrococcus furiosus
0.44
-
 2,3-Butanediol pH 8.8, 45°C, wild-type enzyme, cofactor: NAP+ Pyrococcus furiosus
0.48
-
 NAD+ pH 8.8, 45°C, mutant enzyme G211C Pyrococcus furiosus
0.91
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211S, cofactor: NADP+ Pyrococcus furiosus
1.5
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NADP+ Pyrococcus furiosus
1.6
-
 NAD+ pH 8.8, 45°C, mutant enzyme G211InsS Pyrococcus furiosus
5.5
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NADP+ Pyrococcus furiosus
7.4
-
 NADP+ pH 8.8, 45°C, wild-type enzyme Pyrococcus furiosus
9.6
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211C, cofactor: NADP+ Pyrococcus furiosus
12
-
 2,3-Butanediol pH 8.8, 45°C, wild-type enzyme, cofactor: NAD+ Pyrococcus furiosus
15
-
 NADP+ pH 8.8, 45°C, mutant enzyme G211S Pyrococcus furiosus
17
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NADP+ Pyrococcus furiosus
25
-
 NADP+ pH 8.8, 45°C, mutant enzyme G211C Pyrococcus furiosus
33
-
 NADP+ pH 8.8, 45°C, mutant enzyme G211InsG Pyrococcus furiosus
66
-
 NADP+ pH 8.8, 45°C, mutant enzyme G211InsS Pyrococcus furiosus
76
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NAD+ Pyrococcus furiosus
90
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NADP+ Pyrococcus furiosus
100
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211S, cofactor: NAD+ Pyrococcus furiosus
110
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NAD+ Pyrococcus furiosus
120
-
 NADP+ pH 8.8, 45°C, mutant enzyme G211InsC Pyrococcus furiosus
240
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211C, cofactor: NAD+ Pyrococcus furiosus
260
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NAD+ Pyrococcus furiosus
360
-
 NADP+ pH 8.8, 45°C, mutant enzyme G211InsA Pyrococcus furiosus
1100
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NAD+ Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-butanediol + NAD+
-
 Pyrococcus furiosus acetoin + NADH + H+
-
 ?
2,3-butanediol + NADP+
-
 Pyrococcus furiosus acetoin + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
AdhD
-
 Pyrococcus furiosus
alcohol dehydrogenase D
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.073
-
 NADP+ pH 8.8, 45°C, wild-type enzyme Pyrococcus furiosus
0.073
-
 2,3-Butanediol pH 8.8, 45°C, wild-type enzyme, cofactor: NAP+ Pyrococcus furiosus
0.29
-
 NADP+ pH 8.8, 45°C, mutant enzyme G211S Pyrococcus furiosus
0.29
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211S, cofactor: NADP+ Pyrococcus furiosus
0.43
-
 NAD+ pH 8.8, 45°C, mutant enzyme G211InsC Pyrococcus furiosus
0.43
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NAD+ Pyrococcus furiosus
0.52
-
 NADP+ pH 8.8, 45°C, mutant enzyme G211InsG Pyrococcus furiosus
0.52
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NADP+ Pyrococcus furiosus
0.86
-
 NADP+ pH 8.8, 45°C, mutant enzyme G211InsC Pyrococcus furiosus
0.86
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NADP+ Pyrococcus furiosus
0.92
-
 NADP+ pH 8.8, 45°C, mutant enzyme G211C Pyrococcus furiosus
0.92
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211C, cofactor: NADP+ Pyrococcus furiosus
1.1
-
 NAD+ pH 8.8, 45°C, mutant enzyme G211C Pyrococcus furiosus
1.1
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211C, cofactor: NAD+ Pyrococcus furiosus
1.7
-
 NAD+ pH 8.8, 45°C, mutant enzyme G211InsA Pyrococcus furiosus
1.7
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NAD+ Pyrococcus furiosus
2.1
-
 NADP+ pH 8.8, 45°C, mutant enzyme G211InsA Pyrococcus furiosus
2.1
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NADP+ Pyrococcus furiosus
2.1
-
 NADP+ pH 8.8, 45°C, mutant enzyme G211InsS Pyrococcus furiosus
2.1
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NADP+ Pyrococcus furiosus
2.6
-
 NAD+ pH 8.8, 45°C, wild-type enzyme Pyrococcus furiosus
2.6
-
 2,3-Butanediol pH 8.8, 45°C, wild-type enzyme, cofactor: NAD+ Pyrococcus furiosus
4
-
 NAD+ pH 8.8, 45°C, mutant enzyme G211S Pyrococcus furiosus
4
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211S, cofactor: NAD+ Pyrococcus furiosus
6.5
-
 NAD+ pH 8.8, 45°C, mutant enzyme G211InsG Pyrococcus furiosus
6.5
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NAD+ Pyrococcus furiosus
6.7
-
 NAD+ pH 8.8, 45°C, mutant enzyme G211InsS Pyrococcus furiosus
6.7
-
 2,3-Butanediol pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NAD+ Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 assay at Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pyrococcus furiosus
NADP+
-
 Pyrococcus furiosus