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Literature summary for 1.1.1.1 extracted from

  • Brown, S.; Guss, A.; Karpinets, T.; Parks, J.; Smolin, N.; Yang, S.; Land, M.; Klingeman, D.; Bhandiwad, A.; Rodriguez Jr., M.; Raman, B.; Shao, X.; Mielenz, J.; Smith, J.; Keller, M.; Lynd, L.
    Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum (2011), Proc. Natl. Acad. Sci. USA, 108, 13752-13757.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
structural modeling of ethanol-tolerant mutant protein Acetivibrio thermocellus

Protein Variants

Protein Variants Comment Organism
P704L/H734R mutation leads to ethanol-tolerant phenotype. P704L may affect cofactor-binding directly, while mutation H734R is close to the active site iron atom. Mutant displays a complete loss of NADH-dependent activity with concomitant acquisition of NADPH-dependent activity Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A3DCI2
-
-
Acetivibrio thermocellus DSM 1237 A3DCI2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NADH + H+
-
Acetivibrio thermocellus ethanol + NAD+
-
r
acetaldehyde + NADH + H+
-
Acetivibrio thermocellus DSM 1237 ethanol + NAD+
-
r
acetaldehyde + NADPH + H+ NADPH is not a cofactor for wild-type, but for mutant P704L/H734R Acetivibrio thermocellus ethanol + NADP+
-
r
acetaldehyde + NADPH + H+ NADPH is not a cofactor for wild-type, but for mutant P704L/H734R Acetivibrio thermocellus DSM 1237 ethanol + NADP+
-
r

Synonyms

Synonyms Comment Organism
AdhE
-
Acetivibrio thermocellus
Cthe_0423
-
Acetivibrio thermocellus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Acetivibrio thermocellus
NADPH
-
Acetivibrio thermocellus

General Information

General Information Comment Organism
physiological function in ethanol-tolerant mutant strain, EA, the tolerant phenotype is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE), with a complete loss of NADH-dependent activity and concomitant acquisition of NADPH-dependent activity, which likely affects electron flow in the mutant strain. Mutant adhE allele alone confers increased ethanol tolerance Acetivibrio thermocellus