Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 mM, 123% of initial activity | Yokenella sp. | |
EDTA | 1 mM, 110% of initial activity | Yokenella sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Yokenella sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, no residual activity | Yokenella sp. | |
Co2+ | 1 mM, 19% residual activity | Yokenella sp. | |
Cu2+ | 1 mM, no% residual activity | Yokenella sp. | |
Fe2+ | 1 mM, 60% residual activity | Yokenella sp. | |
Zn2+ | 1 mM, 0.5% residual activity | Yokenella sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
benzaldehyde | pH 6.5, 65°C | Yokenella sp. | |
0.6 | - |
NADPH | pH 6.5, 65°C | Yokenella sp. | |
0.7 | - |
NADP+ | pH 8.0, 55°C | Yokenella sp. | |
3.3 | - |
(2E)-but-2-enal | pH 6.5, 65°C | Yokenella sp. | |
9.1 | - |
(2E)-but-2-en-1-ol | pH 8.0, 55°C | Yokenella sp. | |
14 | - |
benzyl alcohol | pH 8.0, 55°C | Yokenella sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, 113% of initial activity | Yokenella sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36411 | - |
x * 36411, calculated, x * 37000, SDS-PAGE | Yokenella sp. |
37000 | - |
x * 36411, calculated, x * 37000, SDS-PAGE | Yokenella sp. |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | 20% v/v, 24 h, 87% residual activity | Yokenella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yokenella sp. | W6CX26 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Yokenella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-2-methylpent-2-enal + NADPH + H+ | 41.5% of the activity with (2E)-but-2-enal, yield 95% after 12 h | Yokenella sp. | (2E)-2-methylpent-2-en-1-ol + NADP+ | - |
? | |
(2E)-3,7-dimethylocta-2,6-dienal + NADPH + H+ | 37.4% of the activity with (2E)-but-2-enal, yield 87% after 12 h | Yokenella sp. | (2E)-3,7-dimethylocta-2,6-dien-1-ol + NADP+ | - |
? | |
(2E)-but-2-en-1-ol + NADP+ | - |
Yokenella sp. | (2E)-but-2-enal + NADPH + H+ | - |
r | |
(2E)-but-2-enal + NADPH + H+ | yield 96% after 12 h | Yokenella sp. | (2E)-but-2-en-1-ol + NADP+ | - |
r | |
(2E)-dec-2-enal + NADPH + H+ | 16.7% of the activity with (2E)-but-2-enal, yield 91% after 12 h | Yokenella sp. | (2E)-dec-2-en-1-ol + NADP+ | - |
? | |
(2E)-hex-2-enal + NADPH + H+ | 41.7% of the activity with (2E)-but-2-enal, yield 98% after 12 h | Yokenella sp. | (2E)-hex-2-en-1-ol + NADP+ | - |
? | |
(2E)-oct-2-enal + NADPH + H+ | 30.2% of the activity with (2E)-but-2-enal, yield 69% after 12 h | Yokenella sp. | (2E)-oct-2-en-1-ol + NADP+ | plus 27% oct-2-enyl ester | ? | |
benzaldehyde + NADPH + H+ | 107% of the activity with (2E)-but-2-enal, yield 99% after 12 h | Yokenella sp. | benzyl alcohol + NADP+ | - |
r | |
benzyl alcohol + NADP+ | - |
Yokenella sp. | benzaldehyde + NADPH + H+ | - |
r | |
additional information | enzyme selectively reduces the C=O bond of allylic aldehydes/ketones to the corresponding alpha,beta-unsaturated alcohols and also has the capacity of stereoselectively reducing aromatic ketones to (S)-enantioselective alcohols. The enzyme preferentially catalyzes oxidation of allylic/benzyl aldehydes | Yokenella sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36411, calculated, x * 37000, SDS-PAGE | Yokenella sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
oxidation of crotyl aclohol | Yokenella sp. |
65 | - |
reduction of crotonaldehyde | Yokenella sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life 6.2 h | Yokenella sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
93.3 | - |
benzyl alcohol | pH 8.0, 55°C | Yokenella sp. | |
101 | - |
(2E)-but-2-en-1-ol | pH 8.0, 55°C | Yokenella sp. | |
194 | - |
NADP+ | pH 8.0, 55°C | Yokenella sp. | |
360 | - |
benzaldehyde | pH 6.5, 65°C | Yokenella sp. | |
405 | - |
(2E)-but-2-enal | pH 6.5, 65°C | Yokenella sp. | |
433 | - |
NADPH | pH 6.5, 65°C | Yokenella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reduction of crotonaldehyde | Yokenella sp. |
8 | - |
oxidation of crotyl aclohol | Yokenella sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NAD(H) | Yokenella sp. | |
NADPH | enzyme strictly requires NADP(H) as a coenzyme | Yokenella sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.6 | - |
benzyl alcohol | pH 8.0, 55°C | Yokenella sp. | |
11 | - |
(2E)-but-2-en-1-ol | pH 8.0, 55°C | Yokenella sp. | |
122 | - |
(2E)-but-2-enal | pH 6.5, 65°C | Yokenella sp. | |
278 | - |
NADP+ | pH 8.0, 55°C | Yokenella sp. | |
720 | - |
NADPH | pH 6.5, 65°C | Yokenella sp. | |
899 | - |
benzaldehyde | pH 6.5, 65°C | Yokenella sp. |