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Literature summary for 1.1.1.1 extracted from

  • Kawano, S.; Yano, M.; Hasegawa, J.; Yasohara, Y.
    Purification and characterization of an NADH-dependent alcohol dehydrogenase from Candida maris for the synthesis of optically active 1-(pyridyl)ethanol derivatives (2011), Biosci. Biotechnol. Biochem., 75, 1055-1060.
    View publication on PubMed
Search for more literature for 1.1.1.1

Inhibitors

Inhibitors Comment Organism Structure
CoCl2 15% inhibition at 1 mM [Candida] maris
HgCl2 complete inhibition at 1 mM [Candida] maris
iodoacetate 20% inhibition at 1 mM [Candida] maris
MgSO4 19% inhibition at 1 mM [Candida] maris
MnCl2 24% inhibition at 1 mM [Candida] maris
additional information no inhibition by 4-chloromercuribenzoate at 0.1 mM and by CuSO4 at 1 mM, poor effects by EDTA, 1,20-phenanthroline, 2-mercaptoethanol, and DTT [Candida] maris
NEM 17% inhibition at 1 mM [Candida] maris
quercetin 22% inhibition at 0.01 mM [Candida] maris
ZnSO4 26% inhibition at 1 mM [Candida] maris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28900
-
 2 * 28900, SDS-PAGE [Candida] maris
59900
-
 gel filtration [Candida] maris

Organism

Organism UniProt Comment Textmining
[Candida] maris
-
-
-
[Candida] maris IFO10003
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native (R)-specific alcohol dehydrogenase 37fold from strain IFO10003, by ammonium sulfate fractionation, and anion exchange and hydrophobic interaction chromatography to homogeneity [Candida] maris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.6
-
 purified native enzyme, pH 6.5, 30°C [Candida] maris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanal + NADH + H+
-
 [Candida] maris 1-butanol + NAD+
-
 r
1-chloro-5-acetylfuro[2,3-c]pyridine + NADH + H+
-
 [Candida] maris 1-chloro-5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+
-
 r
1-hexanal + NADH + H+
-
 [Candida] maris 1-hexanol + NAD+
-
 r
2,3'-dichloroacetophenone + NADH + H+
-
 [Candida] maris 2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
 r
2,3'-dichloroacetophenone + NADH + H+
-
 [Candida] maris IFO10003 2-chloro-1-(3-chlorophenyl)ethanol + NAD+
-
 r
2-acetylcyclohexanone + NADH + H+
-
 [Candida] maris 2-acetylcyclohexanol + NAD+
-
 r
2-acetylcyclopentanone + NADH + H+
-
 [Candida] maris 2-acetylcyclopentanol + NAD+
-
 r
2-acetylfuran + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
2-acetylpyridine + NADH + H+
-
 [Candida] maris (R)-1-(2-pyridyl)ethanol + NAD+
-
 r
2-acetylpyrrole + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
2-acetylthiazole + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
2-acetylthiophene + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
2-butanone + NADH + H+
-
 [Candida] maris 2-butanol + NAD+
-
 r
2-chloroacetophenone + NADH + H+ low activity [Candida] maris 1-(2-chlorophenyl)ethanol + NAD+
-
 r
2-hexanone + NADH + H+
-
 [Candida] maris 2-hexanol + NAD+
-
 r
2-nitrobenzaldehyde + NADH + H+ low activity [Candida] maris 2-nitrobenzyl alcohol + NAD+
-
 r
2-octanone + NADH + H+
-
 [Candida] maris 2-octanol + NAD+
-
 r
2-oxopentanoate + NADH + H+ low activity [Candida] maris 2-hydroxypentanoate + NAD+
-
 r
2-pentanone + NADH + H+
-
 [Candida] maris 2-pentanol + NAD+
-
 r
3-acetylpyridine + NADH + H+
-
 [Candida] maris (R)-1-(3-pyridyl)ethanol + NAD+
-
 r
3-chlorobenzaldehyde + NADH + H+
-
 [Candida] maris 3-chlorobenzyl alcohol + NAD+
-
 r
3-methylbutan-2-one + NADH + H+
-
 [Candida] maris 3-methyl-2-butanol + NAD+
-
 r
3-nitroacetophenone + NADH + H+
-
 [Candida] maris 1-(3-nitrophenyl)ethanol + NAD+
-
 r
3-nitrobenzaldehyde + NADH + H+
-
 [Candida] maris 3-nitrobenzyl alcohol + NAD+
-
 r
4-acetylpyridine + NADH + H+
-
 [Candida] maris (R)-1-(4-pyridyl)ethanol + NAD+
-
 r
4-chloroacetophenone + NADH + H+
-
 [Candida] maris 1-(4-chlorophenyl)ethanol + NAD+
-
 r
4-chlorobenzaldehyde + NADH + H+
-
 [Candida] maris 4-chlorobenzyl alcohol + NAD+
-
 r
4-fluoroacetophenone + NADH + H+
-
 [Candida] maris 1-(4-fluorophenyl)ethanol + NAD+
-
 r
4-methylpentan-2-one + NADH + H+
-
 [Candida] maris 4-methyl-2-pentanol + NAD+
-
 r
4-nitrobenzaldehyde + NADH + H+
-
 [Candida] maris 4-nitrobenzyl alcohol + NAD+
-
 r
5-acetyl-7-chlorofuro[2,3-c]pyridine + NADH + H+
-
 [Candida] maris 5-(1-hydroxyethyl)-7-chlorofuro[2,3-c]pyridine + NAD+
-
 r
5-acetylfuro[2,3-c]pyridine + NADH + H+
-
 [Candida] maris 5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+
-
 r
acetaldehyde + NADH + H+
-
 [Candida] maris ethanol + NAD+
-
 r
acetaldehyde + NADH + H+
-
 [Candida] maris IFO10003 ethanol + NAD+
-
 r
acetoin + NADH + H+
-
 [Candida] maris 3-hydroxy-2-butanol + NAD+
-
 r
acetone + NADH + H+
-
 [Candida] maris 2-propanol + NAD+
-
 ?
acetophenone + NADH + H+
-
 [Candida] maris 1-phenylethanol + NAD+
-
 r
acetophenone + NADH + H+
-
 [Candida] maris IFO10003 1-phenylethanol + NAD+
-
 r
acetylacetone + 2 NADH + 2 H+
-
 [Candida] maris 2,4-pentanediol + 2 NAD+
-
 r
acetylpyrazine + NADH + H+
-
 [Candida] maris (R)-1-(pyrazyl)ethanol + NAD+
-
 r
benzaldehyde + NADH + H+
-
 [Candida] maris benzyl alcohol + NAD+
-
 r
benzaldehyde + NADH + H+
-
 [Candida] maris IFO10003 benzyl alcohol + NAD+
-
 r
benzylacetone + NADH + H+
-
 [Candida] maris 4-phenylbutan-2-ol + NAD+
-
 r
chloroacetone + NADH + H+
-
 [Candida] maris 1-chloro-2-propanol + NAD+
-
 r
diacetyl + 2 NADH + 2 H+
-
 [Candida] maris 2,3-butandiol + 2 NAD+
-
 r
diethylketone + NADH + H+
-
 [Candida] maris 3-pentanol + NAD+
-
 r
ethyl 4-chloroacetoacetate + NADH + H+
-
 [Candida] maris ethyl 4-chloro-3-hydroxybutanoate + NAD+
-
 r
ethyl acetoacetate + NADH + H+
-
 [Candida] maris ethyl 3-hydroxybutanoate + NAD+
-
 r
ethyl pyruvate + NADH + H+
-
 [Candida] maris ethyl lactate + NAD+
-
 r
methyl acetoacetate + NADH + H+
-
 [Candida] maris methyl 3-hydroxybutanoate + NAD+
-
 r
methyl pyruvate + NADH + H+
-
 [Candida] maris methyl lactate + NAD+
-
 r
additional information substrate specificity and enantiospecificity, overview. The (R)-specific alcohol dehydrogenase requires NADH and reduces various kinds of carbonyl compounds, including ketones and aldehydes. AFPDH reduces acetylpyridine derivatives, beta-keto esters, and some ketones compounds with high enantiospecificity, overview. No activity with 2-chlorobenzaldehyde and 2-tetralone, poor activity with 1-tetralone, pyruvate, 2-oxobutyrate, oxalacetate, cyclopentanone, cyclohexanone, cycloheptanone, and dipropylketone. No activity with 1,2-propanediol, 3-chloro-1,2-propanediol, 3-bromo-1,2-propanediol, glycerol, 1-pentanol, poor activity with 1-butanol, 1-propanol, ethanol, and methanol [Candida] maris ?
-
 ?
additional information substrate specificity and enantiospecificity, overview. The (R)-specific alcohol dehydrogenase requires NADH and reduces various kinds of carbonyl compounds, including ketones and aldehydes. AFPDH reduces acetylpyridine derivatives, beta-keto esters, and some ketones compounds with high enantiospecificity, overview. No activity with 2-chlorobenzaldehyde and 2-tetralone, poor activity with 1-tetralone, pyruvate, 2-oxobutyrate, oxalacetate, cyclopentanone, cyclohexanone, cycloheptanone, and dipropylketone. No activity with 1,2-propanediol, 3-chloro-1,2-propanediol, 3-bromo-1,2-propanediol, glycerol, 1-pentanol, poor activity with 1-butanol, 1-propanol, ethanol, and methanol [Candida] maris IFO10003 ?
-
 ?
oxaloacetate + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
propionaldehyde + NADH + H+
-
 [Candida] maris 1-propanol + NAD+
-
 r
pyridine 2-aldehyde + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
pyridine 3-aldehyde + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r
pyridine 4-aldehyde + NADH + H+
-
 [Candida] maris ? + NAD+
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 28900, SDS-PAGE [Candida] maris

Synonyms

Synonyms Comment Organism
(R)-specific alcohol dehydrogenase
-
 [Candida] maris
AFPDH
-
 [Candida] maris
NADH-dependent alcohol dehydrogenase
-
 [Candida] maris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at [Candida] maris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 75 activity range, profile overview [Candida] maris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 purified native enzyme, stable up to [Candida] maris
40
-
 purified enzyme, inactivation [Candida] maris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 reduction reaction [Candida] maris
8.5
-
 oxidation reaction [Candida] maris

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5 oxidation reaction activity range, profile overview [Candida] maris
5.5 9 reduction reaction activity range, profile overview [Candida] maris

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 [Candida] maris
NADH required [Candida] maris