Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Equus caballus | activity is severely reduced towards aliphatic alcohols of more than 8 carbon atoms for the free enzyme, but not so with immobilized HLAD, exhibiting an activity towards C22 and C24 aliphatic alcohols higher than 50% of the highest value, obtained with C8 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Equus caballus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Equus caballus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanol + NAD+ | immobilized HLAD shows about 95% reaction rate and free HLAD shows about 90% reaction rate with butanol compared to ethanol at pH 8.8 and 30°C | Equus caballus | butyraldehyde + NADH + H+ | - |
? | |
docosanol + NAD+ | immobilized HLAD shows about 60% reaction rate and free HLAD shows about 20% reaction rate with docosanol compared to ethanol at pH 8.8 and 30°C | Equus caballus | ? + NADH + H+ | - |
? | |
dodecanol + NAD+ | immobilized HLAD shows about 60% reaction rate and free HLAD shows about 35% reaction rate with dodecanol compared to ethanol at pH 8.8 and 30°C | Equus caballus | dodecanal + NADH + H+ | - |
? | |
ethanol + NAD+ | immobilized HLAD shows 100% reaction rate and free HLAD shows about 55% reaction rate with ethanol at pH 8.8 and 30°C | Equus caballus | aldehyde + NADH + H+ | - |
? | |
hexadecanol + NAD+ | immobilized HLAD shows about 60% reaction rate and free HLAD shows about 15% reaction rate with hexadecanol compared to ethanol at pH 8.8 and 30°C | Equus caballus | hexadecanal + NADH + H+ | - |
? | |
additional information | activity is severely reduced towards aliphatic alcohols of more than 8 carbon atoms for the free enzyme, but not so with immobilized HLAD, exhibiting an activity towards C22 and C24 aliphatic alcohols higher than 50% of the highest value, obtained with C8 | Equus caballus | ? | - |
? | |
octanol + NAD+ | immobilized and free HLAD show 100% reaction rate with octanol compared to ethanol at pH 8.8 and 30°C | Equus caballus | octanal + NADH + H+ | - |
? | |
tetracosanol + NAD+ | immobilized HLAD shows about 55% reaction rate and free HLAD shows about 15% reaction rate with tetracosanol compared to ethanol at pH 8.8 and 30°C | Equus caballus | ? + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HLAD | - |
Equus caballus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
HLAD is a mesophilic enzyme whose activity and stability are significantly impaired at temperatures over 35°C | Equus caballus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
activity for both free and immobilized HLAD increases with pH up to 8.8 | Equus caballus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Equus caballus |