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Literature summary for 1.1.1.1 extracted from

  • Peng, H.; Wu, G.; Shao, W.
    The aldehyde/alcohol dehydrogenase (AdhE) in relation to the ethanol formation in Thermoanaerobacter ethanolicus JW200 (2008), Anaerobe, 14, 125-127.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermoanaerobacter ethanolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
SDS-PAGE Thermoanaerobacter ethanolicus

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter ethanolicus Q0PH30 strain JW200
-
Thermoanaerobacter ethanolicus JW 200 Q0PH30 strain JW200
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography Thermoanaerobacter ethanolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6
-
in Tris-HCl buffer (pH 7.6), 5 mM dithiothreitol, 0.2 mM NADH, and 10 mM acetaldehyde at 65°C Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + NAD+
-
Thermoanaerobacter ethanolicus acetaldehyde + NADH + H+
-
?
ethanol + NAD+
-
Thermoanaerobacter ethanolicus JW 200 acetaldehyde + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
AdhE the purified AdhE exhibits high enzymatic activity attributed to aldehyde dehydrogenase and low alcohol dehydrogenase activity Thermoanaerobacter ethanolicus
aldehyde/alcohol dehydrogenase
-
Thermoanaerobacter ethanolicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thermoanaerobacter ethanolicus