Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Picrophilus torridus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | 50% (v/v), 82% loss of activity | Picrophilus torridus | |
Al3+ | 10 mM, 30% loss of activity | Picrophilus torridus | |
CHAPS | 10% (w/v), 59% inhibition | Picrophilus torridus | |
DMSO | 50% (v/v), 29% loss of activity | Picrophilus torridus | |
ethanol | 50% (v/v), 59% loss of activity | Picrophilus torridus | |
heptane | 50% (v/v), 99% loss of activity | Picrophilus torridus | |
hexadecane | 50% (v/v), 71% loss of activity | Picrophilus torridus | |
isooctane | 50% (v/v), 98% loss of activity | Picrophilus torridus | |
Isopropanol | 50% (v/v), 88% loss of activity | Picrophilus torridus | |
K+ | 10 mM, 29% loss of activity | Picrophilus torridus | |
methanol | 50% (v/v), 30% loss of activity | Picrophilus torridus | |
Na+ | 10 mM, 13% loss of activity | Picrophilus torridus | |
Ni2+ | 10 mM, 46% loss of activity | Picrophilus torridus | |
pefabloc | 10 mM, 32% inhibition | Picrophilus torridus | |
SDS | 10% (w/v), 95% inhibition | Picrophilus torridus | |
tert-butanol | 50% (v/v), 92% loss of activity | Picrophilus torridus | |
Toluene | 50% (v/v), 97% loss of activity | Picrophilus torridus | |
Triton X-100 | 10% (w/v), 78% inhibition | Picrophilus torridus | |
Tween 20 | 10% (w/v), 82% inhibition | Picrophilus torridus | |
Tween 80 | 10% (w/v), 89% inhibition | Picrophilus torridus | |
Urea | 5 M, 41% inhibition | Picrophilus torridus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
ethanol | - |
Picrophilus torridus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
12 * 35000, SDS-PAGE | Picrophilus torridus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picrophilus torridus | Q6L0S1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Picrophilus torridus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.225 | - |
- |
Picrophilus torridus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phenylethanol + NAD+ | - |
Picrophilus torridus | 1-phenylethanone + NADH | - |
? | |
2,3-butanediol + H2O | - |
Picrophilus torridus | ? | - |
? | |
2-aminoethanol + NAD+ | - |
Picrophilus torridus | ? | - |
? | |
2-propanol + NAD+ | - |
Picrophilus torridus | 2-propanone + NADH | - |
? | |
ethanol + NAD+ | - |
Picrophilus torridus | acetaldehyde + NADH + H+ | - |
? | |
ethanol + NADP+ | 8.5% of the activity with ethanol and NAD+ | Picrophilus torridus | acetaldehyde + NADPH + H+ | - |
? | |
methanol + NAD+ | - |
Picrophilus torridus | formaldehyde + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 35000, SDS-PAGE | Picrophilus torridus |
Synonyms | Comment | Organism |
---|---|---|
AdhA | - |
Picrophilus torridus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
83 | - |
- |
Picrophilus torridus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 90 | 75°C: about 45% of maximal activity, 90°C: about 45% of maximal activity | Picrophilus torridus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
half-life: 114 min | Picrophilus torridus |
40 | - |
half-life: 110 min | Picrophilus torridus |
50 | - |
half-life: 96 min | Picrophilus torridus |
60 | - |
half-life: 55 min | Picrophilus torridus |
70 | - |
half-life: 42 min | Picrophilus torridus |
80 | - |
half-life: 18 min | Picrophilus torridus |
90 | - |
half-life: 4 min | Picrophilus torridus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00086 | - |
ethanol | - |
Picrophilus torridus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Picrophilus torridus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | pH 7: 41% of maximal activity, pH 8: 59% of maximal activity | Picrophilus torridus |