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Literature summary for 1.1.1.1 extracted from

  • Hess, M.; Antranikian, G.
    Archaeal alcohol dehydrogenase active at increased temperatures and in the presence of organic solvents (2008), Appl. Microbiol. Biotechnol., 77, 1003-1013.
    View publication on PubMed
Search for more literature for 1.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Picrophilus oshimae

Inhibitors

Inhibitors Comment Organism Structure
acetone 50% (v/v), 82% loss of activity Picrophilus oshimae
Al3+ 10 mM, 30% loss of activity Picrophilus oshimae
CHAPS 10% (w/v), 59% inhibition Picrophilus oshimae
DMSO 50% (v/v), 29% loss of activity Picrophilus oshimae
ethanol 50% (v/v), 59% loss of activity Picrophilus oshimae
heptane 50% (v/v), 99% loss of activity Picrophilus oshimae
hexadecane 50% (v/v), 71% loss of activity Picrophilus oshimae
isooctane 50% (v/v), 98% loss of activity Picrophilus oshimae
Isopropanol 50% (v/v), 88% loss of activity Picrophilus oshimae
K+ 10 mM, 29% loss of activity Picrophilus oshimae
methanol 50% (v/v), 30% loss of activity Picrophilus oshimae
Na+ 10 mM, 13% loss of activity Picrophilus oshimae
Ni2+ 10 mM, 46% loss of activity Picrophilus oshimae
pefabloc 10 mM, 32% inhibition Picrophilus oshimae
SDS 10% (w/v), 95% inhibition Picrophilus oshimae
tert-butanol 50% (v/v), 92% loss of activity Picrophilus oshimae
Toluene 50% (v/v), 97% loss of activity Picrophilus oshimae
Triton X-100 10% (w/v), 78% inhibition Picrophilus oshimae
Tween 20 10% (w/v), 82% inhibition Picrophilus oshimae
Tween 80 10% (w/v), 89% inhibition Picrophilus oshimae
Urea 5 M, 41% inhibition Picrophilus oshimae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
 ethanol
-
 Picrophilus oshimae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 12 * 35000, SDS-PAGE Picrophilus oshimae

Organism

Organism UniProt Comment Textmining
Picrophilus oshimae Q6L0S1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Picrophilus oshimae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.225
-
-
 Picrophilus oshimae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phenylethanol + NAD+
-
 Picrophilus oshimae 1-phenylethanone + NADH
-
 ?
2,3-butanediol + H2O
-
 Picrophilus oshimae ?
-
 ?
2-aminoethanol + NAD+
-
 Picrophilus oshimae ?
-
 ?
2-propanol + NAD+
-
 Picrophilus oshimae 2-propanone + NADH
-
 ?
ethanol + NAD+
-
 Picrophilus oshimae acetaldehyde + NADH + H+
-
 ?
ethanol + NADP+ 8.5% of the activity with ethanol and NAD+ Picrophilus oshimae acetaldehyde + NADPH + H+
-
 ?
methanol + NAD+
-
 Picrophilus oshimae formaldehyde + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
dodecamer 12 * 35000, SDS-PAGE Picrophilus oshimae

Synonyms

Synonyms Comment Organism
AdhA
-
 Picrophilus oshimae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
83
-
-
 Picrophilus oshimae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
75 90 75°C: about 45% of maximal activity, 90°C: about 45% of maximal activity Picrophilus oshimae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 half-life: 114 min Picrophilus oshimae
40
-
 half-life: 110 min Picrophilus oshimae
50
-
 half-life: 96 min Picrophilus oshimae
60
-
 half-life: 55 min Picrophilus oshimae
70
-
 half-life: 42 min Picrophilus oshimae
80
-
 half-life: 18 min Picrophilus oshimae
90
-
 half-life: 4 min Picrophilus oshimae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00086
-
 ethanol
-
 Picrophilus oshimae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Picrophilus oshimae

pH Range

pH Minimum pH Maximum Comment Organism
7 8 pH 7: 41% of maximal activity, pH 8: 59% of maximal activity Picrophilus oshimae