Application | Comment | Organism |
---|---|---|
biotechnology | possible usage of the enzyme in bioindustrial processes and as biosensor | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
overexpression as GST-fusion protein in Escherichia coli | Geobacillus stearothermophilus |
General Stability | Organism |
---|---|
highly stable against 0.1 M urea and 0.05% SDS | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
imidazole | - |
Geobacillus stearothermophilus | |
Pyridine | - |
Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.11 | - |
ethanol | - |
Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
reombinant fusion enzyme by glutathione affinity chromatography, cleavage of GST fusion tag by thrombin, further purification of the active enzyme | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + NAD+ | - |
Geobacillus stearothermophilus | acetaldehyde + NADH | - |
? | |
additional information | broad substrate specificity | Geobacillus stearothermophilus | ? | - |
? | |
secondary alcohol + NAD+ | - |
Geobacillus stearothermophilus | aldehyde + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
recombinant enzyme | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | - |
purified recombinant enzyme, most stable at | Geobacillus stearothermophilus |
85 | - |
remaining activity | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant enzyme | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Geobacillus stearothermophilus |