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Literature summary for 1.1.1.1 extracted from

  • Shim, E.J.; Jeon, S.H.; Kong, K.H.
    Overexpression, purification, and biochemical characterization of the thermostable NAD-dependent alcohol dehydrogenase from Bacillus stearothermophilus (2003), J. Microbiol. Biotechnol., 13, 738-744.
No PubMed abstract available

Application

Application Comment Organism
biotechnology possible usage of the enzyme in bioindustrial processes and as biosensor Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
overexpression as GST-fusion protein in Escherichia coli Geobacillus stearothermophilus

General Stability

General Stability Organism
highly stable against 0.1 M urea and 0.05% SDS Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
imidazole
-
Geobacillus stearothermophilus
Pyridine
-
Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.11
-
ethanol
-
Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
reombinant fusion enzyme by glutathione affinity chromatography, cleavage of GST fusion tag by thrombin, further purification of the active enzyme Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + NAD+
-
Geobacillus stearothermophilus acetaldehyde + NADH
-
?
additional information broad substrate specificity Geobacillus stearothermophilus ?
-
?
secondary alcohol + NAD+
-
Geobacillus stearothermophilus aldehyde + NADH
-
?

Synonyms

Synonyms Comment Organism
ADH
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
recombinant enzyme Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
68
-
purified recombinant enzyme, most stable at Geobacillus stearothermophilus
85
-
remaining activity Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
recombinant enzyme Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Geobacillus stearothermophilus