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Literature summary for 1.1.1.1 extracted from

  • Hildebrandt, P.; Musidlowska, A.; Bornscheuer, U.T.; Altenbuchner, J.
    Cloning, functional expression and biochemical characterization of a stereoselective alcohol dehydrogenase from Pseudomonas fluorescens DSM50106 (2002), Appl. Microbiol. Biotechnol., 59, 483-487.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli using a rhamnose-inducible system Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31997
-
 x * 31997, amino acid sequence calculation Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
 strain DSM 50106, stereoselective enzyme
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetophenone + NADH the enzyme exhibits high stereoselectivity in the desymmetrization of the prochiral ketone acetophenone, producing optically pure (R)-alpha-phenyl ethanol at high conversion Pseudomonas fluorescens (R)-alpha-phenyl ethanol + NAD+
-
 ?
cyclohexanone + NADH best cyclic ketone substrate Pseudomonas fluorescens cyclohexanol + NAD+
-
 ?

Subunits

Subunits Comment Organism
? x * 31997, amino acid sequence calculation Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas fluorescens

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Pseudomonas fluorescens