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Literature summary for 1.1.1.1 extracted from
- Characterization of mutation-induced changes in the maize (Zea mays L.) ADH1-1S1108 alcohol dehydrogenase (1993), Biochem. Genet., 31, 497-506.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.037 | - |
NAD+ | wild-type enzyme ADH1-1S | Zea mays |  |
| 0.038 | - |
NAD+ | mutant enzyme ADH1-1S1108 | Zea mays | |
| 0.042 | - |
NADH | mutant enzyme ADH1-1S1108 | Zea mays | |
| 0.085 | - |
NADH | wild-type enzyme ADH1-1S | Zea mays | |
| 22 | - |
ethanol | mutant enzyme ADH1S-1108 | Zea mays | |
| 24 | - |
ethanol | wild-type enzyme ADH1-1S | Zea mays | |
| 70 | - |
acetaldehyde | mutant enzyme ADH1-1S1108 | Zea mays | |
| 85 | - |
acetaldehyde | wild-type enzyme ADH1-1S | Zea mays | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
wild-type enzyme Adh1-1S and mutant enzyme Adh1-1S1108 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type enzyme Adh1-1S and mutant enzyme Adh1-1S1108 | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kernel | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 65 | - |
mutant enzyme Adh1-1S1108 | Zea mays |
| 70 | - |
wild-type enzyme Adh1-1S | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylpropan-1-ol + NAD+ | - |
Zea mays | 2-methyl-propan-1-one + NADH | - |
? | |
| allyl alcohol + NAD+ | - |
Zea mays | prop-2-en-1-al + NADH | - |
? | |
| butan-2-ol + NAD+ | no activity | Zea mays | butan-2-one + NADH | - |
? | |
| butanol + NAD+ | - |
Zea mays | butyraldehyde + NADH | - |
? | |
| ethanol + NAD+ | - |
Zea mays | acetaldehyde + NADH | - |
? | |
| hexan-1-ol + NAD+ | - |
Zea mays | n-hexanal + NADH | - |
? | |
| propan-2-ol + NAD+ | - |
Zea mays | acetone + NADH | - |
? | |
| propanol + NAD+ | - |
Zea mays | propionaldehyde + NADH + H+ | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
wild-type enzyme Adh1-1S is more stable than mutant enzyme Adh1-1S1108 | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
mutant enzyme ADH1-1S1108 | Zea mays |
| 8.5 | - |
wild-type enzyme ADH1-1S | Zea mays |
| 9 | - |
oxidation of ethanol | Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Zea mays | |
| NADH | - |
Zea mays | |
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