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Literature summary for 1.1.1.1 extracted from

  • Ditlow, C.C.; Holmquist, B.; Morelock, M.M.; Vallee, B.L.
    Physical and enzymatic properties of a class II alcohol dehydrogenase isozyme of human liver: pi-ADH (1984), Biochemistry, 23, 6363-6368.
    View publication on PubMed
Search for more literature for 1.1.1.1

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Homo sapiens
2,2'-bipyridine
-
 Homo sapiens
4-Methylpyrazole poor inhibitor, class II isoenzyme Homo sapiens
EDTA
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 benzyl alcohol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
0.007
-
 Octanol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
0.032
-
 3-Phenyl-1-propanol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
0.034
-
 Vanillyl alcohol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
0.06
-
 16-hydroxyhexadecanoate 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
0.09
-
 Pentanol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
0.2
-
 tryptophol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
0.23
-
 12-hydroxydodecanoate 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
120
-
 ethanol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
210
-
 Cyclohexanol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
290
-
 ethylene glycol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
310
-
 2-deoxy-D-ribose 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
560
-
 2-propanol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 class II isoenzyme: pi-ADH
-

Purification (Commentary)

Purification (Comment) Organism
class II isoenzyme: pi-ADH Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.47
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
4°C, pH 7.5, stable for 2-3 weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12-hydroxydodecanoate + NAD+
-
 Homo sapiens 12-oxododecanoic acid + NADH
-
 ?
16-hydroxyhexadecanoate + NAD+
-
 Homo sapiens 16-oxohexadecanoic acid + NADH
-
 ?
2-deoxy-D-ribose + NAD+
-
 Homo sapiens ? + NADH
-
 ?
3-phenyl-1-propanol + NAD+
-
 Homo sapiens 3-phenyl-1-propanone + NADH
-
 ?
benzyl alcohol + NAD+
-
 Homo sapiens benzaldehyde + NADH
-
 ?
cyclohexanol + NAD+
-
 Homo sapiens cyclohexanone + NADH
-
 ?
ethanol + NAD+
-
 Homo sapiens acetaldehyde + NADH
-
 ?
ethylene glycol + NAD+
-
 Homo sapiens ? + NADH
-
 ?
methanol + NAD+ activity detected with class II isoenzyme pi-ADH Homo sapiens formaldehyde + NADH + H+
-
 ?
octan-1-ol + NAD+
-
 Homo sapiens n-octanal + NADH
-
 ?
pentanal + NAD+
-
 Homo sapiens pentanone + NADH
-
 ?
propan-2-ol + NAD+
-
 Homo sapiens acetone + NADH
-
 ?
tryptophol + NAD+
-
 Homo sapiens ? + NADH
-
 ?
vanillyl alcohol + NAD+
-
 Homo sapiens vanillin + NADH
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.583
-
 Cyclohexanol
-
 Homo sapiens
0.75
-
 2-propanol
-
 Homo sapiens
0.75
-
 ethylene glycol
-
 Homo sapiens
1.83
-
 tryptophol
-
 Homo sapiens
2.83
-
 2-deoxy-D-ribose
-
 Homo sapiens
4
-
 12-hydroxydodecanoate
-
 Homo sapiens
6.17
-
 16-hydroxyhexadecanoate
-
 Homo sapiens
7.5
-
 3-Phenyl-1-propanol
-
 Homo sapiens
7.83
-
 ethanol
-
 Homo sapiens
8
-
 Pentanol
-
 Homo sapiens
8.33
-
 Octanol
-
 Homo sapiens
8.67
-
 Vanillyl alcohol
-
 Homo sapiens
9.17
-
 benzyl alcohol
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.4
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
8 12 about 30% of maximal activity at pH 8.0 and at pH 12.0 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens
NADH
-
 Homo sapiens