Ligand 1,3-1,4-beta-D-glucan
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Basic Ligand Information
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (3 results)
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1,3-1,4-beta-D-glucan + H2O = ?
-
1,3-1,4-beta-D-glucan + H2O = cellooligosaccharide
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1,3-1,4-beta-D-glucan + H2O = ?
-
Enzyme Kinetic Parameters
kcat Value (Turnover Number) (6 results)
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114.8
-
pH 5, 50°C, soluble, from barley, EG VII
281.2
-
pH 5, 50°C, soluble, from barley, EG VI
2.25
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wild-type, expressed in Escherichia coli, pH 5.0, 50°C
2.44
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mutant V18Y, expressed in Escherichia coli, pH 5.0, 50°C
3.29
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mutant V18Y/W203Y, expressed in Escherichia coli, pH 5.0, 50°C
3.67
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mutant W203Y, expressed in Escherichia coli, pH 5.0, 50°C
References & Links
Literature References (3)
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A transglycosylating 1,3(4)-beta-glucanase from Rhodothermus marinus. NMR analysis of enzyme reactions
2000
Petersen, B.O.; Krah, M.; Duus, J.O.; Thomsen, K.K.
Eur. J. Biochem.
267
361-369
Catalytic properties and mode of action of three endo-beta-glucanases from Talaromyces emersonii on soluble beta-1,4- and beta-1,3;1,4-linked glucans
2003
McCarthy, T.; Hanniffy, O.; Savage, A.V.; Tuohy, M.G.
Int. J. Biol. Macromol.
33
141-148
Rational design to improve thermostability and specific activity of the truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase
2012
Huang, J.W.; Cheng, Y.S.; Ko, T.P.; Lin, C.Y.; Lai, H.L.; Chen, C.C.; Ma, Y.; Zheng, Y.; Huang, C.H.; Zou, P.; Liu, J.R.; Guo, R.T.
Appl. Microbiol. Biotechnol.
94
111-121
Links to other databases for 1,3-1,4-beta-D-glucan
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