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Ligand 2-Hydroxy-(4-chlorophenyl)-acetonitrile Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information Molecular Structure
C8 H6 ClNO
2-Hydroxy-(4-chlorophenyl)-acetonitrile
LSDSHEPNJSMUTI-UHFFFAOYSA-N
(4-chlorophenyl)(hydroxy)acetonitrile, (R)-2-hydroxy-2-(4-chlorophenyl)acetonitrile, 4-chloromandelonitrile
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (2 results)
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4-chloromandelonitrile + H2O = 3-chloromandelic acid + NH3
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(4-chlorophenyl)(hydroxy)acetonitrile + H2O = (2R)-(4-chlorophenyl)(hydroxy)acetic acid + NH3
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Product in Enzyme-catalyzed Reactions (1 result)
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HCN + 4-chlorbenzaldehyde = (R)-2-hydroxy-2-(4-chlorophenyl)acetonitrile
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (2 results)
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2.2
-
pH 7.5, 40°C, wild-type enzyme
3.07
-
pH 7.5, 40°C, mutant enzyme S190G
KM Value (2 results)
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6.08
-
pH 7.5, 40°C, mutant enzyme S190G
6.15
-
pH 7.5, 40°C, wild-type enzyme
References & Links Literature References (2)
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Cloning, purification and evaluation of the enzymatic properties of a novel arylacetonitrilase from Luminiphilus syltensis NOR5-1B: a potential biocatalyst for the synthesis of mandelic acid and its derivatives
2015
Sun, H.; Gao, W.; Fan, H.; Wang, H.; Wei, D.
Biotechnol. Lett.
37
1655-1661
Improving catalytic performance of an arylacetonitrilase by semirational engineering
2017
Xue, Y.P.; Jiao, B.; Hua, D.E.; Cheng, F.; Liu, Z.Q.; Zheng, Y.G.
Bioprocess Biosyst. Eng.
40
1565-1572
Links to other databases for 2-Hydroxy-(4-chlorophenyl)-acetonitrile