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Ligand dihexanoylphosphatidylcholine Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information Molecular Structure
C2 0 H4 0 NO8 P
dihexanoylphosphatidylcholine
DVZARZBAWHITHR-UHFFFAOYSA-O
1,2-dicaproyl-sn-glycero-3-phosphocholine, 1,2-Dihexanoylphosphatidylcholine, dihexanoylglycerophosphocholine, dihexanoyl phosphatidylcholine
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (2 results)
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dihexanoylphosphatidylcholine + H2O = dihexanoylglycerol + phosphorylcholine
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dihexanoylphosphatidylcholine + H2O = dihexanoylglycerophosphate + choline
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Product in Enzyme-catalyzed Reactions (1 result)
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CDP-choline + dihexanoylglycerol = CMP + dihexanoylglycerophosphocholine
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Activator in Enzyme-catalyzed Reactions (4 results)
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i.e. C6PC, binding constants in presence of Ca2+
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activates, 4-5fold increase in catalytic efficiency, binds to a lipid-binding subsite, not to the active site, maximal activation at 0.4 mM
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non-substrate activator lipid, maximum PI-PLC activity at 0.7-1 mM
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (2 results)
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monomeric substrate, recombinant PLC
4.93
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monomeric substrate, wild-type PLC
6.78
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KM Value (3 results)
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monomeric substrate, recombinant PLC
1.12
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monomeric substrate, wild-type PLC
0.19
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References & Links Literature References (7)
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Engineering of the nonspecific phospholipase C from Bacillus cereus: Replacement of glutamic acid-4 by alanine results in loss of interfacial catalysis and enhanced phosphomonoesterase activity
1998
Tan, C.A.; Roberts, M.F.
Biochemistry
37
4275-4279
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Phospholipase D from savoy cabbage
1969
Yang, S.F.
Methods Enzymol.
14
208-210
Phospholipase D from savoy cabbage: purification and preliminary kinetic characterization
1979
Allgyer, T.T.; Wells, M.A.
Biochemistry
18
5348-5353
The inhibition and activation of Ca2+-dependent phosphatidylinositol phosphodiesterase by phospholipids and blood plasma
1980
Dawson, R.M.C.; Hemington, N.; Irvine, R.F.
Eur. J. Biochem.
112
33-38
Specificity of soluble phospholipid binding sites on human factor Xa
2002
Banerjee, M.; Drummond, D.C.; Srivastava, A.; Daleke, D.; Lentz, B.R.
Biochemistry
41
7751-7762
Listeria monocytogenes phosphatidylinositol-specific phospholipase C: Activation and allostery
2002
Ryan, M.; Zaikova, T.O.; Keana, J.F.W.; Goldfine, H.; Griffith, O.H.
Biophys. Chem.
101-102
347-358
Allosteric interactions within subsites of a monomeric enzyme: Kinetics of fluorogenic substrates of PI-specific phospholipase C
2003
Birrell, G.B.; Zaikova, T.O.; Rukavishnikov, A.V.; Keana, J.F.W.; Griffith, O.H.
Biophys. J.
84
3264-3275
Links to other databases for dihexanoylphosphatidylcholine
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