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Ligand (1,4-alpha-D-galacturonide)2

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Substrate (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.67

(1,4-alpha-D-galacturonide)2 + H2O = D-galacturonic acid

136785, 136789, 136784, 136786, 671022, 114101, 668557, 671023, 136796, 136776, 136777, 136778, 136782

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Product in Enzyme-catalyzed Reactions (1 result)

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Show Product (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.67

(1,4-alpha-D-galacturonide)5 + H2O = D-galacturonic acid + (1,4-alpha-D-galacturonide)2 + (1,4-alpha-D-galacturonide)3 + (1,4-alpha-D-galacturonide)4

136776, 0

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Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (2 results)

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Show Turnover Number (2 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.2.1.67

0.17

-

0.1 M acetate buffer pH 3.8, 30°C

671022

3.2.1.67

182

-

30 mM phosphate buffer pH 6.5, 80°C

668557

3.2.1.67

2 entries

K_M Value (5 results)

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Show KM Value (5 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.2.1.67

0.34

-

30 mM phosphate buffer pH 6.5, 80°C

668557

3.2.1.67

0.86

-

0.1 M acetate buffer pH 3.8, 30°C

671022

3.2.1.67

1.44

-

-

136796

3.2.1.67

2.2

2.8

-

136786

3.2.1.67

2.24

-

-

136778

3.2.1.67

5 entries

References & Links

Literature References (13)

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Show Reference (13 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

114101

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Purification and characterization of pectinesterase and polygalacturonase from Trichoderma reesei

1985

Markovic, O.; Slezarik, A.; Labudova, I.

FEMS Microbiol. Lett.

27

267-271

136776

-

Exopolygalacturonase in tomato fruit

1987

Pressey, R.

Phytochemistry

26

1867-1870

136777

-

Immobilization of exo-D-galacturonanase by coupling to a polyacrylamide type support

1986

Heinrichova, K.; Lehoczki, M.; Zliechovcova, D.

J. Gen. Microbiol.

131

2291-2298

136778

4056742

An exo-D-galacturonanase of Butyrivibrio fibrisolvens from the bovine rumen

1985

Heinrichova, K.; Wojciechowicz, M.; Ziolecki, A.

J. Gen. Microbiol.

131

2053-2058

136782

-

Purification and a partial characterization of exo-D-galacturonanase of banana

1982

Heinrichova, K.

Collect. Czech. Chem. Commun.

47

2433-2439

136784

-

Isolation, characterization and mode of action of exo-D-galacturonanase from carrot

1977

Heinrichova, K.

Collect. Czech. Chem. Commun.

42

3214-3221

136785

2310

Purification and characterization of an extracellular exo-D-galacturonanase of Aspergillus niger

1976

Heinrichova, K.; Rexova-Benkova, L.

Biochim. Biophys. Acta

422

349-356

136786

-

Modes of action of carrot and peach exopolygalacturonases

1975

Pressey, R.; Avants, J.K.

Phytochemistry

14

957-961

136789

5661621

Isolation of an oligogalacturonate hydrolase from a Bacillus species

1968

Hasegawa, S.; Nagel, C.W.

Arch. Biochem. Biophys.

124

513-520

136796

8856078

Primary structure and characterization of an exopolygalacturonase from Aspergillus tubingensis

1996

Kester, H.C.M.; Kusters-Van Someren, M.A.; Mueller, Y.; Visser, J.

Eur. J. Biochem.

240

738-746

668557

16262687

Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima

2005

Kluskens, L.D.; van Alebeek, G.J.; Walther, J.; Voragen, A.G.; de Vos, W.M.; van der Oost, J.

FEBS J.

272

5464-5473

671022

16402551

Oligogalacturonate hydrolase from carrot roots

2006

Stratilova, E.; Dzurova, M.; Malovikova, A.; Omelkova, J.

Z. Naturforsch. C

60

899-905

671023

15787251

Purification and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans

2005

Stratilova, E.; Dzurova, M.; Breierova, E.; Omelkova, J.

Z. Naturforsch. C

60

91-96

Links to other databases for (1,4-alpha-D-galacturonide)2

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ChEBI

PubChem

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