Ligand cortisol

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

Basic Ligand Information

Molecular Structure
Picture of cortisol (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C21H30O5
cortisol
JYGXADMDTFJGBT-VWUMJDOOSA-N
Synonyms:
4-pregnene-11beta,17alpha,21-triol-3,20-dione, 11beta,17alpha,21-trihydroxy-4-pregnen-3,20-dione, 11beta,17alpha,21-trihydroxy-4-pregnene-3,20-dione, 11beta,17alpha,21-trihydroxy-pregn-4-en-3,20-dione, 11beta,17alpha,21-trihydroxy-pregn-4-ene-3,20-dione, 11beta-cortisol, 11beta-hydrocortisone, 17alpha-hydroxycorticosterone, hydrocorticosterone, hydrocortisone

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
cortisol + reduced adrenal ferredoxin + O2 = 11-deoxycortisol + adrenal ferredoxin + H2O
show the reaction diagram
-
cortisol + NADPH = ?
show the reaction diagram
-
acetyl-CoA + cortisol = CoA + cortisol 21-acetate
show the reaction diagram
-
3'-phosphoadenylylsulfate + cortisol = adenosine 3',5'-bisphosphate + cortisol 21-sulfate
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (8 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
cortisone + NADPH = cortisol + NADP+
show the reaction diagram
-
-

Substrate in Enzyme-catalyzed Reactions (29 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
11beta,17alpha,21-trihydroxy-4-pregnen-3,20-dione + NADPH = 3beta,11beta,17alpha,21-tetrahydroxy-4-pregnen-20-one + NADP+
show the reaction diagram
-
Cortisol + NADPH + O2 = ?
show the reaction diagram
-
11beta-hydrocortisone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = 9alpha-hydroxy-11beta-hydrocortisone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
show the reaction diagram
-
acetyl-CoA + cortisol = CoA + cortisol 21-acetate
show the reaction diagram
-
UDP-alpha-D-glucose + hydrocortisone = UDP + 11beta,17-dihydroxy-3,20-dioxopregn-4-en-21-yl beta-D-glucopyranoside
show the reaction diagram
-
3'-phosphoadenylylsulfate + cortisol = adenosine 3',5'-bisphosphate + cortisol sulfate
show the reaction diagram
-
3'-phosphoadenylylsulfate + cortisol = adenosine 3',5'-bisphosphate + cortisol 21-sulfate
show the reaction diagram
-
3'-phosphoadenylyl 5'-phosphosulfate + cortisol = adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
3'-phosphoadenylylsulfate + hydrocortisone = adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (14 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
11,17-dihydroxyprogesterone + NADH + O2 = cortisol + NAD+ + H2O
show the reaction diagram
-

Activator in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
induces
-
treatment of hepatocytes induces the expression of only the heavy enzyme subunit
-

Inhibitor in Enzyme-catalyzed Reactions (11 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
i.e. cortisol
-
competitive
-
3 mM, 5% inhibition
-
inhibition above 0.08 mM
-

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (7 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.183
-
pH 7.5, 37°C

KM Value (39 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0038
-
-
0.032
-
NADH, pH 6.3
0.00682
-
pH 6.8, 37.5°C
0.012
-
pH 7, 25°C

Ki Value (1 result)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.19
-
androsterone oxidation

IC50 Value (1 result)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.017
-
in 0.1 M potassium phosphate, pH 7.4, at 25°C

References & Links

Links to other databases for cortisol

ChEBI
PubChem
-
PubChem