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Ligand Isoprimeverose

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Product in Enzyme-catalyzed Reactions (3 results)

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Show Natural Product (3 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.120

oligoxyloglucan + H2O = isoprimeverose + ?

0

-

3.2.1.120

xyloglucan + H2O = isoprimeverose + ?

0

-

3.2.1.120

xyloglucan fragment oligosaccharide + H2O = isoprimeverose

646669

-

3.2.1.120

3 entries

Substrate in Enzyme-catalyzed Reactions (5 results)

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Show Substrate (5 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.2

isoprimeverose + starch = ?

659632

-

3.2.1.120

alpha-D-Xyl-(1->6)-beta-D-Glc + H2O = ?

751081

-

3.2.1.120

Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + isoprimeverose = Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc

680407

-

3.2.1.177

isoprimeverose + H2O = alpha-D-xylose + beta-D-glucose

724946, 715011, 716825, 715446, 714102

-

3.2.1.177

isoprimeverose + H2O = D-xylose + D-glucose

754214

-

3.2.1.120

2 entries

3.2.1.177

2 entries

Product in Enzyme-catalyzed Reactions (5 results)

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Show Product (5 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.207

[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid = Xyl(alpha1-6)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid

0

-

3.2.1.120

alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O = alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc

0

-

3.2.1.120

oligoxyloglucan + H2O = isoprimeverose + ?

0

-

3.2.1.120

xyloglucan + H2O = isoprimeverose + ?

0

-

3.2.1.120

xyloglucan fragment oligosaccharide + H2O = isoprimeverose

0, 646669, 646671

-

3.2.1.120

4 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (4 results)

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Show Turnover Number (4 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.2.1.120

532

-

pH 4.5, 60°C

751081

3.2.1.177

2.94

-

pH 6, 25°C

714102

3.2.1.177

7.69

-

pH 5.5, 45°C, recombinant enzyme

754214

3.2.1.177

51.3

-

pH 7.0, 37°C

716825

3.2.1.177

3 entries

K_M Value (4 results)

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Show KM Value (4 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.2.1.120

4.03

-

pH 4.5, 60°C

751081

3.2.1.177

0.55

-

pH 7.0, 37°C

716825

3.2.1.177

8

9

pH 5.5, 45°C, recombinant enzyme

754214

3.2.1.177

55

-

pH 6, 25°C

714102

3.2.1.177

3 entries

References & Links

Literature References (11)

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Show Reference (11 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

646669

4019436

A novel enzyme producing isoprimeverose from oligoxyloglucans of Aspergillus oryzae

1985

Kato, Y.; Matsushita, J.; Kubodera, T.; Matsuda, K.

J. Biochem.

97

801-810

646671

12223734

A xyloglucan-specific endo-1,4-beta-glucanase isolated from auxin-treated pea stems

1997

Matsumoto, T.; Sakai, F.; Hayashi, T.

Plant Physiol.

114

661-667

659632

15973532

Dextran dextrinase and dextran of Gluconobacter oxydans

2005

Naessens, M.; Cerdobbel, A.; Soetaert, W.; Vandamme, E.J.

J. Ind. Microbiol. Biotechnol.

32

323-334

680407

-

Screening, purification and characterization of a prokaryotic isoprimeverose-producing oligoxyloglucan hydrolase from Oerskovia sp. Y1

2007

Yaoi, K.; Hiyoshi, A.; Mitsuishi, Y.

J. Appl. Glycosci.

54

91-94

714102

21426303

Structural and enzymatic characterisation of a glycoside hydrolase family 31 alpha-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification

2011

Larsbrink, J.; Izumi, A.; Ibatullin, F.; Nakhai, A.; Gilbert, H.J.; Davies, G.J.; Brumer, H.

Biochem. J.

436

567-580

715011

16631751

Structural elements to convert Escherichia coli alpha-xylosidase (YicI) into alpha-glucosidase

2006

Okuyama, M.; Kaneko, A.; Mori, H.; Chiba, S.; Kimura, A.

FEBS Lett.

580

2707-2711

715446

10801892

Identification and molecular characterization of the first alpha-xylosidase from an archaeon

2000

Moracci, M.; Cobucci Ponzano, B.; Trincone, A.; Fusco, S.; De Rosa, M.; van Der Oost, J.; Sensen, C.W.; Charlebois, R.L.; Rossi, M.

J. Biol. Chem.

275

22082-22089

716825

15294295

Overexpression and characterization of two unknown proteins, YicI and YihQ, originated from Escherichia coli

2004

Okuyama, M.; Mori, H.; Chiba, S.; Kimura A.

Protein Expr. Purif.

37

170-179

724946

11523897

Enzymatic synthesis and hydrolysis of xylogluco-oligosaccharides using the first archaeal alpha-xylosidase from Sulfolobus solfataricus

2001

Trincone, A.; Cobucci-Ponzano, B.; Di Lauro, B.; Rossi, M.; Mitsubishi, Y.; Moracci, M.

Extremophiles

5

277-282

751081

26755723

Identification of the gene encoding isoprimeverose-producing oligoxyloglucan hydrolase in Aspergillus oryzae

2016

Matsuzawa, T.; Mitsuishi, Y.; Kameyama, A.; Yaoi, K.

J. Biol. Chem.

291

5080-5087

754214

27074950

Screening, identification, and characterization of alpha-xylosidase from a soil metagenome

2016

Matsuzawa, T.; Kimura, N.; Suenaga, H.; Yaoi, K.

J. Biosci. Bioeng.

122

393-399

Links to other databases for Isoprimeverose

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Show Links (1 entry)

ChEBI

PubChem