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Ligand bis-4-nitrophenyl phosphate

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Basic Ligand Information

Molecular Structure
Picture of bis-4-nitrophenyl phosphate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C12H9N2O8P
bis-4-nitrophenyl phosphate
MHSVUSZEHNVFKW-UHFFFAOYSA-N
Synonyms:
4-bis-nitrophenyl phosphate, bis(4-nitrophenyl) phosphate, bis(4-nitrophenyl)phosphate, bis(4-nitrophenyl) phosphoric acid, bis(p-nitro-phenyl)phosphate, bis(p-nitrophenyl)-phosphate, bis(p-nitrophenyl)phosphate, bis(p-nitrophenyl) phosphate, bis(p-nitrophenylphosphate), bis(p-nitrophenyl phosphate), bis(para-nitrophenyl)phosphate, bis-(4-nitrophenyl)phosphate, bis-(4-nitrophenyl) phosphate, bis-(4-nitrophenylphosphate), bis-(p-nitrophenyl)phosphate, bis-(p-nitrophenyl) phosphate, bis-4-nitrophenylphosphate, bis-p-nitrophenylphosphate, bis-p-nitrophenyl phosphate, bis-p nitrophenyl phosphate, bis p-nitrophenylphosphate, di(p-nitrophenyl) phosphate, di-p-nitrophenyl phosphate

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (57 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
bis-(4-nitrophenyl)phosphate + H2O = 4-nitrophenyl phosphate + 4-nitrophenol
show the reaction diagram
-
bis(p-nitrophenyl) phosphate + H2O = p-nitrophenol + p-nitrophenyl phosphate:
show the reaction diagram
-
bis(p-nitrophenyl)phosphate + H2O = p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
-
bis(p-nitrophenyl)phosphate + H2O = ?
show the reaction diagram
-
bis(4-nitrophenyl) phosphate + H2O = ?
show the reaction diagram
-
bis-p-nitrophenylphosphate + H2O = ?
show the reaction diagram
-
bis(p-nitrophenyl)phosphate + H2O = p-nitrophenol + phosphate
show the reaction diagram
-
bis-(4-nitrophenyl) phosphate + H2O = ?
show the reaction diagram
-
bis-4-nitrophenyl phosphate + H2O = ?
show the reaction diagram
-
bis-(4-nitrophenyl) phosphate + H2O = 4-nitrophenyl phosphate + 4-nitrophenol
show the reaction diagram
-
bis-(4-nitrophenyl) phosphate + H2O = ?
show the reaction diagram
-
bis(p-nitrophenyl) phosphate + H2O = p-nitrophenyl phosphate + p-nitrophenol
show the reaction diagram
-
bis(p-nitrophenyl)phosphate + H2O = ?
show the reaction diagram
-
bis(4-nitrophenyl) phosphate + H2O = 4-nitrophenyl phosphate + 4-nitrophenol
show the reaction diagram
-
bis(4-nitrophenyl) phosphate + H2O = 4-nitrophenyl phosphate + 4-nitrophenol
show the reaction diagram
-
bis(4-nitrophenyl)phosphate + H2O = ?
show the reaction diagram
-

Inhibitor in Enzyme-catalyzed Reactions (32 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
weak inhibitor
-
0.01 mM, 8% inhibition of lysophospholipase I and 61% inhibition of lysophospholipase II
-
little effect on the activity
-
pre-tRNA processing inhibited by bis(p-nitrophenyl)phosphate concentrations higher than 10 mM observed
-
inhibition above a concentration of 1 mM
-
1 mM, competitive inhibition
-
slight inhibition at 1 mM after 30 min preincubation
-

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (84 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
700
-
-
0.033
-
pH 7.3, 22°C, recombinant enzyme
0.21
-
-
6
-
-

KM Value (115 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
37
-
pH 7.3, 22°C, recombinant enzyme
0.28
-
-
14.5
-
-
0.4
-
-
0.9
-
-
0.16
-
-
0.6
-
pH 8.0, 0.065 mM Ca2+
0.4
-
-

Ki Value (1 result)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
2.31
-
pH 5.0, 30°C

IC50 Value (5 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE

References & Links

Links to other databases for bis-4-nitrophenyl phosphate

ChEBI
PubChem
ChEBI
PubChem