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Ligand o-toluidine

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (7 results)

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Show Substrate (7 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.10.3.2

4 toluidine + O2 = ?

726534

-

1.10.3.2

o-toluidine + O2 = ?

742692, 764340

-

1.10.3.2

2 entries

1.11.1.7

o-toluidine + H2O2 = ? + H2O

658234

-

2.3.1.5

acetyl-CoA + 2-methylaniline = CoA + N-acetyl-2-methylaniline

686074

-

2.3.1.5

acetyl-CoA + 2-toluidine = CoA + N-acetyl-2-toluidine

687848, 675406

-

2.3.1.5

2 entries

2.5.1.2

thiamine + 2-methylbenzeneamine = 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-methylphenyl)amine

636851

-

6.3.1.18

ATP + L-glutamate + 2-methylaniline = ?

729932

-

Product in Enzyme-catalyzed Reactions (3 results)

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Show Product (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.7.1.16

2-nitrotoluene + 3 NADPH + 3 H+ = 2-toluidine + 3 NADP+ + 2 H2O

-

-

3.1.1.1

prilocaine + H2O = o-toluidine + N-propylalanine

-

-

3.5.1.9

2-methylformanilide + H2O = 2-methylaniline + formate

-

-

Activator in Enzyme-catalyzed Reactions (1 result)

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Show Activating Compound (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

3.5.1.9

activates

743358

-

3D Structure of Enzyme-Ligand-Complex (PDB) (2 results)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (2 entries)

EC NUMBER

ENZYME 3D STRUCTURE

1.11.1.5

5CID, 3D-view

3.4.23.22

5DPZ, 3D-view

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (6 results)

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Show Turnover Number (6 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.11.1.7

270

-

pH 7.0

658234

2.3.1.5

4.8

-

pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate

675406

2.3.1.5

6.3

-

pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate

675406

2.3.1.5

12

-

-

686074

2.3.1.5

72

-

pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate

675406

2.3.1.5

111

-

-

686074

2.3.1.5

5 entries

K_M Value (7 results)

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Show KM Value (7 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.11.1.7

2.5

-

pH 7.0

658234

2.3.1.5

1.1

-

pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate

675406

2.3.1.5

1.2

-

pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate

675406

2.3.1.5

2.32

-

-

686074

2.3.1.5

6.6

-

pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate

675406

2.3.1.5

7.18

-

-

686074

2.3.1.5

5 entries

2.5.1.2

6.4

-

-

636851

References & Links

Literature References (12)

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Show Reference (12 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

636851

-

The purification and properties of a thiaminase I enzyme from nardoo (Marsilea Drumondii)

1977

McCleary, B.V.; Chick, B.F.

Phytochemistry

16

207-213

658234

15493874

Kinetic characterization of phenol and aniline derivates as substrates of peroxidase

2004

Gilabert, M.A.; Fenoll, L.G.; Garcia-Molina, F.; Tudela, J.; Garcia-Canovas, F.; Rodriguez-Lopez, J.N.

Biol. Chem.

385

795-800

675406

16959263

NMR-based model reveals the structural determinants of mammalian arylamine N-acetyltransferase substrate specificity

2006

Zhang, N.; Liu, L.; Liu, F.; Wagner, C.R.; Hanna, P.E.; Walters, K.J.

J. Mol. Biol.

363

188-200

686074

17672512

Arylamine N-acetyltransferases: characterization of the substrate specificities and molecular interactions of environmental arylamines with human NAT1 and NAT2

2007

Liu, L.; Von Vett, A.; Zhang, N.; Walters, K.J.; Wagner, C.R.; Hanna, P.E.

Chem. Res. Toxicol.

20

1300-1308

687848

17368397

Bacillus cereus strain 10-L-2 produces two arylamine N-acetyltransferases that transform 4-phenylenediamine into 4-aminoacetanilide

2007

Mulyono, R.; Takenaka, S.; Sasano, Y.; Murakami, S.; Aoki, K.

J. Biosci. Bioeng.

103

147-154

726534

23712478

Purification and biochemical characterization of a new alkali-stable laccase from Trametes sp. isolated in Tunisia: role of the enzyme in olive mill waste water treatment

2013

Daassi, D.; Zouari-Mechichi, H.; Prieto, A.; Martinez, M.J.; Nasri, M.; Mechichi, T.

World J. Microbiol. Biotechnol.

29

2145-2155

729932

23893114

Function of a glutamine synthetase-like protein in bacterial aniline oxidation via gamma-glutamylanilide

2013

Takeo, M.; Ohara, A.; Sakae, S.; Okamoto, Y.; Kitamura, C.; Kato, D.; Negoro, S.

J. Bacteriol.

195

4406-4414

742692

27793681

Purification, characterization, and cloning of an extracellular laccase with potent dye decolorizing ability from white rot fungus Cerrena unicolor GSM-01

2017

Wang, S.S.; Ning, Y.J.; Wang, S.N.; Zhang, J.; Zhang, G.Q.; Chen, Q.J.

Int. J. Biol. Macromol.

95

920-927

742692

27793681

Purification, characterization, and cloning of an extracellular laccase with potent dye decolorizing ability from white rot fungus Cerrena unicolor GSM-01

2017

Wang, S.S.; Ning, Y.J.; Wang, S.N.; Zhang, J.; Zhang, G.Q.; Chen, Q.J.

Int. J. Biol. Macromol.

95

920-927

742692

2 entries

743358

26970015

Kynurenine pathway metabolites and enzymes involved in redox reactions

2017

Gonzalez Esquivel, D.; Ramirez-Ortega, D.; Pineda, B.; Castro, N.; Rios, C.; Perez de la Cruz, V.

Neuropharmacology

112

331-345

764340

32282952

Purification and properties of a laccase from the mushroom Agaricus sinodeliciosus

2021

Hao, J.; Zhang, W.; Wang, H.; Ziya, N.; Luo, Y.; Jia, P.; Zhang, G.; Ng, T.

Biotechnol. Appl. Biochem.

68

297-306

764340

32282952

Purification and properties of a laccase from the mushroom Agaricus sinodeliciosus

2021

Hao, J.; Zhang, W.; Wang, H.; Ziya, N.; Luo, Y.; Jia, P.; Zhang, G.; Ng, T.

Biotechnol. Appl. Biochem.

68

297-306

764340

2 entries

Links to other databases for o-toluidine

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