Ligand dihydroorotate

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

Basic Ligand Information

Molecular Structure
Picture of dihydroorotate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C5H6N2O4
dihydroorotate
UFIVEPVSAGBUSI-UHFFFAOYSA-N
Synonyms:
D-dihydroorotate, dihydro-DL-orotate, dihydroorotic acid
Pathway Source
Pathways


Show all pahtways known for Show all BRENDA pathways known for dihydroorotate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
dihydroorotate + NAD+ = orotate + NADH + H+
-
show the reaction diagram
dihydroorotate + acceptor = orotate + reduced acceptor
-

Substrate in Enzyme-catalyzed Reactions (36 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
dihydroorotic acid + H2O = ?
-

Product in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE

Inhibitor in Enzyme-catalyzed Reactions (3 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
10 mM
-
slight inhibition
-
competitive inhibition
-

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (10 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
-
50.4
-
full-length DHODH
2.1
-
mutant F188A
12.3
-
mutant H185A
1.4
-
mutant R265K
16.1
-
N-terminally truncated DHODH
2.2
-
pH 8.0, 30°C, DELTA1-75 mutant
72.9
-
pH 8.0, 30°C, wild-type
8.2
-
wild-type
15.8
-
-
127
-

KM Value (40 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
-
0.009
-
wild-type recombinant enzyme
0.017
-
wild-type
0.013
-
recombinant enzyme
0.015
-
pH 8.0, 30°C, wild-type
0.04
-
pH 8.0, 30°C, DELTA1-75 mutant
0.121
-
N-terminally truncated DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of decylubiquinone from 0-0.2 mM
0.065
-
N-terminally truncated DHODH, fixed concentration of dihydroorotate and varied concentrations of 2,6-dichloroindophenol from 0-0.2 mM
0.04
-
N-terminally truncated DHODH, concentration of dihydroorotate varied from 0-1.0 mM at fixed concentrations of 0.1 mM decylubiquinone and 0.06 mM 2,6-dichloroindophenol
0.111
-
mutant R265K
0.013
-
mutant H185A
0.019
-
mutant F188A
0.017
-
full-length DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of decylubiquinone from 0-0.2 mM
0.042
-
full-length DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of 2,6-dichloroindophenol from 0-0.2 mM
0.122
-
full-length DHODH, concentration of dihydroorotate varied from 0-1.0 mM at fixed concentrations of 0.1 mM decylubiquinone and 0.06 mM 2,6-dichloroindophenol
0.108
-
DELTA22-37 mutant enzyme
0.0166
-
DELTA2-37 mutant enzyme
0.0167
-
DELTA2-21 mutant enzyme
0.0164
-
-
0.005
-
cosubstrate ubiquinone-50
0.004
-
cosubstrate decylubiquinone
0.009
-
pH 8.5, 70°C
0.0442
-
native DHOD at 25°C
0.0259
-
37°C, pH 7.0
DHOD at 25°C
0.0243
-
in the presence of 1 mM fumarate
0.0579
-
in the presence of 0.5 mM fumarate
0.0472
-
in the presence of 0.25 mM fumarate
0.0332
-
in the presence of 0.125 mM fumarate
0.0192
-
DHOD3 at 37°C
0.071
-
DHOD1 and DHOD2 at 37°C
0.03
-
Zn/Zn-dihydroorotase, pH 8.0
0.08
-
purified recombinant enzyme, at 37°C
0.063
-
Cd/Cd-dihydroorotase, pH 8.0
0.23
-
Co/Co-dihydroorotase, pH 8.0
0.7
-

IC50 Value (1 result)

COMMENTARY
EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
LITERATURE
pH 8.0, 25°C, recombinant enzyme
18.6
-

References & Links

Links to other databases for dihydroorotate

ChEBI
PubChem
-
PubChem