Ligand Br-

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Basic Ligand Information

Molecular Structure
Picture of Br- (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
HBr
Br-
CPELXLSAUQHCOX-UHFFFAOYSA-M
Synonyms:
bromide, HBr, Hydrobromate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
Br- + H2O2 + H+ = HOBr + H2O
show the reaction diagram
-
L-tryptophan + FADH2 + Br- + O2 + H+ = 5-bromo-L-tryptophan + FAD + 2 H2O
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
monobromoacetate + H2O = glycolate + HBr
show the reaction diagram
-
-
monobromoacetate + H2O = 2-hydroxyacetate + bromide
show the reaction diagram
-
-

Substrate in Enzyme-catalyzed Reactions (210 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione = ?
show the reaction diagram
-
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione = ?
show the reaction diagram
-
phenol + bromide = 2-bromophenol + 4-bromophenol
show the reaction diagram
-
1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + 2 FADH2 + 2 bromide + 2 O2 = 4,5-dibromo-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + 2 FAD + 4 H2O
show the reaction diagram
-
L-tryptophan + FADH2 + Br- + O2 + H+ = 5-bromo-L-tryptophan + FAD + 2 H2O
show the reaction diagram
-
indole + FADH2 + bromide + O2 + H+ = 3-bromoindole + FAD + 2 H2O
show the reaction diagram
-
S-(3S)-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + 2 FADH2 + 2 H+ + Br- + O2 = S-(3S)-3-bromo-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + FAD + 2 H2O
show the reaction diagram
-
12-epi-fischerindole U + 2-oxoglutarate + O2 + bromide = 13R-bromo-12-epi-fischerindole U + succinate + CO2 + H2O
show the reaction diagram
-
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Br- = 4-bromo-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O
show the reaction diagram
-
S-adenosyl-L-methionine + bromide = S-adenosyl-L-homocysteine + methyl bromide
show the reaction diagram
-
S-adenosyl-L-methionine + bromide = S-adenosyl-L-homocysteine + bromomethane
show the reaction diagram
-
S-adenosyl-L-methionine + bromide = 5'-deoxy-5'-bromoadenosine + L-methionine
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (416 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
KBr + 2 H2O = KH + HBr + H2O2
show the reaction diagram
-
-
2-bromobenzoate + NADH + O2 = catechol + bromide + NAD+ + CO2
show the reaction diagram
-
2,2'-dibromobiphenyl + NADH + O2 = 2,3-dihydroxy-2'-bromobiphenyl + NAD+ + HBr
show the reaction diagram
-
-
4-bromophenylacetate + NADH + O2 + H2O = 3,4-dihydroxyphenylacetate + Br- + NAD+
show the reaction diagram
-
-
2,4,6-tribromophenol + NADPH + O2 = 2,6-dibromo-p-hydroquinone + Br- + NADP+ + H2O
show the reaction diagram
-
2-chloro-4-bromophenol + FADH2 + O2 = 2-chlorobenzoquinone + Br- + FAD + H2O
show the reaction diagram
-
-
3-bromo-L-tyrosine + NADPH + H+ = L-tyrosine + NADP+ + Br-
show the reaction diagram
-
-
5-deoxy-5'-bromoadenosine + fluoride = 5-deoxy-5'-fluoroadenosine + bromide
show the reaction diagram
-
-
5-deoxy-5'-bromoadenosine + fluoride = 5-deoxy-5'-fluoroadenosine + bromide
show the reaction diagram
-
-
p-coumaric acid + 2-phenylethyl bromide = 2-phenylethyl p-coumarate + bromide
show the reaction diagram
-
-
L-Lys-L-Ala-5-chloro-1-anthraquinonylhydrazide-2HBr + H2O = L-Lys-L-Ala + 5-Cl-1-anthraquinonylhydrazine + HBr
show the reaction diagram
-
6-bromopurine + H2O = hypoxanthine + HBr
show the reaction diagram
-
monobromoacetate + H2O = glycolate + HBr
show the reaction diagram
-
-
4-bromobenzoate + H2O = 4-hydroxybenzoate + Br-
show the reaction diagram
-
p-(bromomethyl)mandelate = p-(methyl)benzoylformate + Br-
show the reaction diagram
-

Activator in Enzyme-catalyzed Reactions (14 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
0.6 M, maximal stimulation
-
activates NAD:arginine ADP-ribosyltransferase A
-
stimulation at low concentrations, inhibition above 200 mM
-
stimulates alanine transamination, inhibits lysine transamination
-
1.6fold induction
-
activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate
-
greater affinity for the enzyme than chloride and a comparable relative activity
-
stimulates aminopeptidase activity
-
activates

Inhibitor in Enzyme-catalyzed Reactions (60 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
competitive, at low pH
-
marked inhibition at 1 mM
-
inhibition of mutant enzyme S402A between pH 3.5 and 4.5: concentrations greater than 0.2 mM inhibit, between pH 5.0 and 5.5: concentrations above 0.5 mM inhibit, between pH 6.0 and 6.3: 0.5 mM does not inhibit the mutant enzyme
-
competitive versus I-
-
37% inactivation at 0.1 M
-
50% inhibition at 0.072 M
-
addition of any of the neutral salts causes a parabolic inhibition. A direct comparison of arsenate and chloride ion shows that arsenate is not as inhibitory even though it has a higher ionic strength. Arsenate interacts with the enzyme differently from chloride and bromide. Sulfate, which is a large ion like arsenate, is as inhibitory as chloride (at equivalent ionic strength) with NAD+ as coenzyme, but it is much less effective than chloride with NADP+ as coenzyme
-
competitive vs. cytochrome b5, reversible by dilution
-
above 0.02 M
-
inhibition above 200 mM, stimulation below
-
93.33% residual activity at 5 mM
-
stimulates alanine transamination, inhibits lysine transamination
-
noncompetitive to glucose
-
same inhibition as I-, stronger inhibition than Cl-, the kind of cation has no inhibitory effect
-
inhibitory at 20 mM
-
50 mM for 50% inhibition
-
competitive
-
noncompetitive
-
0.1 M, 50% inhibition
1.2 M, 50% inhibition of ATP-diphosphate exchange
-

Metals and Ions (26 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
stimulates luminescence activity
-
strong stimulation
-
retards the oxidative half-reaction
-
activates
-
activates
-
activates both isozymes TAH I and TAH II
-
activates
-
activates
-
activates by 10% at 10 mg/ml
-
stimulates peptidase and epoxide hydrolase activity (i.e. conversion of leukotriene A4 into leukotriene B4), SCN- most effective, followed by Cl- and Br-
-
4 mM, activation
-
activation
-
activation
-
2 mM, activation
-
stimulation by anions with the following order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme
-

3D Structure of Enzyme-Ligand-Complex (PDB) (866 results)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (10 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.46
-
20°C, pH 6.5
0.013
-
in 50 mM phosphate buffer (pH 7.9) at 37°C

KM Value (36 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
4.4
-
-
2
-
in 100 mM phosphate buffer, pH 7.4, at 21°C
1.32
-
pH 7.2, 30°C
177.3
-
in 20 mM MES (pH 7.0), at 30°C
150
-
in 50 mM phosphate buffer (pH 7.9) at 37°C

Ki Value (22 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
102
-
pH 7.5, 25°C
65
-
-
0.1
-
-

IC50 Value (2 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE

References & Links