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pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O
pentalenolactone F + O2 + NADPH + H+ = pentalenolactone + NADP+ + H2O
pentalenolactone F + O2 + NADPH + H+ = pentalenolactone + NADP+ + H2O
pentalenolactone F + O2 + NADPH + H+ = pentalenolactone + NADP+ + H2O
pentalenolactone F + O2 + NADPH + H+ = pentalenolactone + NADP+ + H2O
pentalenolactone F + O2 + NADPH + H+ = pentalenolactone + NADP+ + H2O
pentalenolactone F + O2 + NADPH + H+ = pentalenolactone + NADP+ + H2O
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pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O
pentalenolactone F + O2 + NADPH + H+ = pentalenolactone + NADP+ + H2O
pentalenolactone F + O2 + NADPH + H+ = pentalenolactone + NADP+ + H2O
pentalenolactone F + O2 + NADPH + H+ = pentalenolactone + NADP+ + H2O
pentalenolactone F + O2 + NADPH + H+ = pentalenolactone + NADP+ + H2O
pentalenolactone F + O2 + NADPH + H+ = pentalenolactone + NADP+ + H2O
pentalenolactone F + O2 + NADPH + H+ = pentalenolactone + NADP+ + H2O
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0.000067
-
recombinant mutant M81A, pH 8.0, 30°C
0.00032
-
recombinant mutant M77S, pH 8.0, 30°C
0.00058
-
recombinant mutant F232A, pH 8.0, 30°C
0.00142
-
recombinant mutant M81C-2-mercaptoethanol conjugate, pH 8.0, 30°C
0.0015
-
recombinant mutant F232L, pH 8.0, 30°C
0.00153
-
recombinant mutant M81C, pH 8.0, 30°C
0.03
-
recombinant wild-type enzyme, pH 8.0, 30°C
8.8
-
at 30°C, pH not specified in the publication
10.5
-
at 30°C, pH not specified in the publication
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0.012
-
recombinant mutant F232L, pH 8.0, 30°C
0.019
-
recombinant mutant M77S, pH 8.0, 30°C
0.019
-
recombinant mutant M81C, pH 8.0, 30°C
0.036
-
recombinant wild-type enzyme, pH 8.0, 30°C
0.037
-
recombinant mutant M81A, pH 8.0, 30°C
0.043
-
recombinant mutant F232A, pH 8.0, 30°C
0.28
-
recombinant mutant M81C-2-mercaptoethanol conjugate, pH 8.0, 30°C
0.34
-
at 30°C, pH not specified in the publication
0.43
-
at 30°C, pH not specified in the publication
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A gene cluster for biosynthesis of the sesquiterpenoid antibiotic pentalenolactone in Streptomyces avermitilis
2006
Tetzlaff, C.N.; You, Z.; Cane, D.E.; Takamatsu, S.; Omura, S.; Ikeda, H.
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Genome mining in Streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone
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J. Am. Chem. Soc.
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The cytochrome P450-catalyzed oxidative rearrangement in the final step of pentalenolactone biosynthesis substrate structure determines mechanism
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J. Am. Chem. Soc.
138
12678-12689
Oxidative rearrangement mechanism of pentalenolactone F catalyzed by cytochrome P450 CYP161C2 (PntM)
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