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Ligand pseudooxynicotine

Basic Ligand Information

Molecular Structure

Related pathways

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Natural Substrate (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.4.2.3

pseudooxynicotine + H2O + 2 ferricytochrome c protein CycN = 4-oxo-4-(pyridin-3-yl)butanal + methylamine + 2 ferrocytochrome c protein CycN + 2 H+

767147, 724032

-

1.4.2.3

pseudooxynicotine + H2O + ? = 4-oxo-4-(pyridin-3-yl)butanal + methylamine + ?

768010

-

1.4.2.3

2 entries

In Vivo Product in Enzyme-catalyzed Reactions (1 result)

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Show Natural Product (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.5.99.4

(S)-nicotine + acceptor + H2O = pseudooxynicotine + reduced acceptor

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-

Substrate in Enzyme-catalyzed Reactions (3 results)

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Show Substrate (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.4.2.3

4-(methylamino)-1-(pyridin-3-yl)butan-1-one + H2O + 2 ferricytochrome c = 4-oxo-4-(pyridin-3-yl)butanal + methylamine + 2 ferrocytochrome c + 2 H+

718644

-

1.4.2.3

pseudooxynicotine + H2O + 2 ferricytochrome c protein CycN = 4-oxo-4-(pyridin-3-yl)butanal + methylamine + 2 ferrocytochrome c protein CycN + 2 H+

767147, 724032

-

1.4.2.3

pseudooxynicotine + H2O + ? = 4-oxo-4-(pyridin-3-yl)butanal + methylamine + ?

768010

-

1.4.2.3

3 entries

Product in Enzyme-catalyzed Reactions (3 results)

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Show Product (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.5.3.6

(R)-nicotine + H2O + O2 = 1-(pyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2

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-

1.5.99.4

(RS)-nicotine + acceptor + H2O = pseudooxynicotine + reduced acceptor

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-

1.5.99.4

(S)-nicotine + acceptor + H2O = pseudooxynicotine + reduced acceptor

-

-

1.5.99.4

2 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (2 results)

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Show Turnover Number (2 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.4.2.3

0.79

-

at pH 8.5 and 30°C

768010

1.4.2.3

151

-

pH 8.0, temperature not specified in the publication

718644

1.4.2.3

2 entries

K_M Value (2 results)

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Show KM Value (2 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.4.2.3

0.073

-

at pH 8.5 and 30°C

768010

1.4.2.3

0.247

-

pH 8.0, temperature not specified in the publication

718644

1.4.2.3

2 entries

References & Links

Literature References (4)

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Show Reference (4 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

718644

22267672

Functional identification of two novel genes from Pseudomonas sp. strain HZN6 involved in the catabolism of nicotine

2012

Qiu, J.; Ma, Y.; Wen, Y.; Chen, L.; Wu, L.; Liu, W.

Appl. Environ. Microbiol.

78

2154-2160

724032

23335761

Cloning of a novel nicotine oxidase gene from Pseudomonas sp. strain HZN6 whose product nonenantioselectively degrades nicotine to pseudooxynicotine

2013

Qiu, J.; Ma, Y.; Zhang, J.; Wen, Y.; Liu, W.

Appl. Environ. Microbiol.

79

2164-2171

767147

35835223

The enzyme pseudooxynicotine amine oxidase from Pseudomonas putida S16 is not an oxidase, but a dehydrogenase

2022

Choudhary, V.; Wu, K.; Zhang, Z.; Dulchavsky, M.; Barkman, T.; Bardwell, J.; Stull, F.

J. Biol. Chem.

298

102251

768010

26634650

Characterization of pseudooxynicotine amine oxidase of Pseudomonas putida S16 that is crucial for nicotine degradation

2015

Hu, H.; Wang, W.; Tang, H.; Xu, P.

Sci. Rep.

5

17770

Links to other databases for pseudooxynicotine

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Show Links (1 entry)