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Ligand alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (7 results)

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Show Substrate (7 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.15

alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O = alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate

135820, 135824, 135818, 135813, 135853, 135834

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3.2.1.15

alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O = alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + galacturonate

654804

-

3.2.1.15

pentagalacturonate + H2O = trigalacturonate + digalacturonate + tetragalacturonate + galacturonate

749784

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3.2.1.15

3 entries

3.2.1.67

[(1->4)-alpha-D-galacturonide]5 + H2O = [(1->4)-alpha-D-galacturonide]4 + D-galacturonate

747730

-

3.2.1.82

alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronosyl-(1->4)-alpha-D-galactopyranuronic acid + H2O = ?

136895

-

4.2.2.2

pentagalacturonic acid = saturated digalacturonic acid + unsaturated trigalacturonic acid + saturated trigalacturonic acid + unsaturated digalacturonic acid

37369, 37382, 37367, 37371

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4.2.2.9

pentagalacturonic acid = ?

34118, 34115

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Product in Enzyme-catalyzed Reactions (2 results)

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Show Product (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.15

alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O = alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate

0

-

3.2.1.67

[(1->4)-alpha-D-galacturonide]6 + H2O = [(1->4)-alpha-D-galacturonide]5 + D-galacturonate

0

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Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (3 results)

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Show Turnover Number (3 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.2.1.15

35.8

-

at pH 4.0 and 30°C

749784

3.2.1.15

81.1

-

-

654804

3.2.1.15

2 entries

3.2.1.67

934

-

pH 6.5, 80°C

747730

K_M Value (10 results)

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Show KM Value (10 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.2.1.15

0.000052

-

-

654804

3.2.1.15

0.0365

-

at pH 4.0 and 30°C

749784

3.2.1.15

0.71

-

-

135813

3.2.1.15

0.87

-

-

135818

3.2.1.15

0.89

-

-

135820

3.2.1.15

1.85

-

-

135824

3.2.1.15

4.8

-

-

135834

3.2.1.15

7 entries

3.2.1.67

0.24

-

pH 6.5, 80°C

747730

3.2.1.82

3.1

-

-

136895

4.2.2.2

0.23

-

-

37369

References & Links

Literature References (16)

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Show Reference (16 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

34115

-

Exopolygalacturonate lyase produced by Streptomyces massasporeus

1980

Sato, M.; Kaji, A.

Agric. Biol. Chem.

44

717-721

34118

10049400

Characterization of the exopolygalacturonate lyase PelX of Erwinia chrysanthemi 3937

1999

Shevchik, V.E.; Kester, H.C.M.; Benen, J.A.E.; Visser, J.; Robert-Baudouy, J.; Hugouvieux-Cotte-Pattat, N.

J. Bacteriol.

181

1652-1663

37367

-

Purification and properties of pectate lyase produced by Streptomyces fradiae IFO 3439

1975

Sato, M.; Kaji, A.

Agric. Biol. Chem.

39

819-824

37369

-

Purification and properties of pectate lyase produced by Streptomyces nitrosporeus

1977

Sato, M.; Kaji, A.

Agric. Biol. Chem.

41

2193-2197

37371

-

Another pectate lyase produced by Streptomyces nitrosporeus

1980

Sato, M.; Kaji, A.

Agric. Biol. Chem.

44

1345-1349

37382

6035509

Polygalacturonic acid trans-eliminase of Xanthomonas campestris

1967

Nasuno, S.; Starr, M.P.

Biochem. J.

104

178-185

135813

-

Purification and some properties of a protopectin-solubilizing enzyme from Galactomyces reessii strain L

1984

Sakai, T.; Yoshitake, S.

Agric. Biol. Chem.

48

1941-1950

135818

-

Some properties of endo-polygalacturonase from Trichosporon penicillatum SNO-3

1982

Sakai, T.; Okushima, M.; Sawada, M.

Agric. Biol. Chem.

46

2223-2231

135820

-

Purification, characterization and mode of effect of another endo-D-galacturonase from Aspergillus niger

1981

Heinrichova, K.; Dzurova, M.

Collect. Czech. Chem. Commun.

46

3145-3156

135824

-

Purification and characterization of another D-galacturonase from Aspergillus niger

1977

Heinrichova, K.; Rexova-Benkova, L.

Collect. Czech. Chem. Commun.

42

2569-2576

135834

-

Properties of a polygalacturonase produced by Acrocylindrium

1973

Kimura, H.; Uchino, F.; Mizushima, S.

J. Gen. Microbiol.

74

127-137

135853

-

Purification and characterization of an endopolygalacturonase from Physalospora piricola

1994

Miyairi, K.; Matsue, T.; Kagawa, O.; Kutsuzawa, T.; Okuno, T.

Biosci. Biotechnol. Biochem.

58

1909-1910

136895

8481009

Isolation and characterisation of an extracellular glycosylated protein complex from Clostridium thermosacchalyticum with pectin methylesterase and polygalacturonate hydrolase activity

1993

Van Rijssel, M.; Gerwig, G.J.; Hansen, T.A.

Appl. Environ. Microbiol.

59

828-836

654804

11410340

Study of the mode of action of endopolygalacturonase from Fusarium moniliforme

2001

Bonnin, E.; Le Goff, A.; Korner, R.; Van Alebeek, G.W.; Christensen, T.M.; Voragen, A.G.; Roepstorff, P.; Caprari, C.; Thibault, J.

Biochim. Biophys. Acta

1526

301-309

747730

16262687

Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima

2005

Kluskens, L.; Van Alebeek, G.; Walther, J.; Voragen, A.; De Vos, W.; Van Der Oost, J.

FEBS J.

272

5464-5473

749784

-

Kinetic properties of Rhizopus oryzae RPG1 endo-polygalacturonase hydrolyzing galacturonic acid oligomers

2016

Mertens, J.; Bowman, M.

Biocatal. Agricult. Biotechnol.

5

11-16

Links to other databases for alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate

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ChEBI

PubChem

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