Ligand phenylmethanesulfonyl fluoride

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Basic Ligand Information

Molecular Structure

C₇H₇FO₂S

phenylmethanesulfonyl fluoride

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

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Synonyms:

(phenylmethyl)sulfonyl fluoride, C6H5CH2SO3F, p-methylsulfonylfluoride, phenyl-methanesulfonyl fluoride, phenyl-methyl-sulfonyl-fluoride, phenylmethane-sulfonylfluoride, phenylmethanesulfonly fluoride, phenylmethanesulfonylflouride, Phenylmethanesulfonylfluoride, phenylmethane sulfonyl fluoride, phenylmethane sulfonylfluoride, phenyl methane sulfonyl fluoride, Phenylmethanesulphonyl fluoride, phenylmethanosulfonyl fluorate, Phenylmethyl-sulfonyl fluoride, phenylmethylsufonyl fluoride, phenylmethylsulfonic fluoride, phenylmethylsulfonyl-fluoride, phenylmethylsulfonyl fluoride, phenyl methylsulfonyl fluoride, phenyl methyl sulfonylfluoride, phenyl methyl sulfonyl fluoride, phenyl methylsulfonylfluoride, Phenylmethyl sulfonylfluoride, phenylmethyl sulfonyl fluoride, Phenylmethylsulfonylfluoride, Phenylmethylsulphonylfluoride, Phenylmethylsulphonyl fluoride, phenyl methyl sulphonyl fluoride, PMSF

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (2 results)

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1.3.8.6

glutaryl-CoA + phenylmethylsulfonyl fluoride = ?

391409

3.5.1.86

phenylmethylsulfonyl fluoride + H2O = ?

680440

Activator in Enzyme-catalyzed Reactions (31 results)

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1.1.1.73

111% activity at 1 mM

739937

1.11.1.9

2 mM, 167% of initial activity

764056

1.4.3.2

exposure of the enzyme to phenylmethylsulfonyl fluoride results in the LAAO expressing anticoagulant activity, preventing contact activation generated thrombin from forming a clot

slightly activating

1 mM + 1 mM DTT, activation to 147% of control

657589

2.4.1.19

80 mM, relative activity 114%

0.1 mM: inhibition of intracellular proteolytic proteins during the incubation of disrupted yeast cells for RNA hydrolysis

134104

3.1.3.26

0.1 mM, 63% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified

activates 14% at 5 mM

5 mM, 28% increase in enzyme activity

776028

3.2.1.67

113.77% activity at 5 mM

750600

3.2.1.8

activates xylanase I, inhibits xylanase II

707211

3.2.1.B33

5 mM, 1.1fold activation

725057

3.2.1.B50

activates 14% at 5 mM

activates

weak activation

5 mM, 1.2fold activation

719130

3.5.1.13

slight activation at 1 mM

733190

3.5.1.138

123% activity at 0.5 mM

769105

3.5.1.4

slight activation at 1 mM

1 mM, 40% inhibition

1 mm, 126% of initial activity

747385

Inhibitor in Enzyme-catalyzed Reactions (917 results)

63% inhibition at 10 mM

721272

1.1.1.184

about 10% inhibition at 1 mM

655017

1.1.1.2

lower molecular weight form

286177

1.1.1.202

85% inhibition at 5 mM

about 48% inhibition at 5 mM

740402

1.1.1.79

1 mM, 30% inhibition, incubation for 1 min

287318

1.1.1.8

0.5 mM 50% inhibition of cytosolic form, little effect on chloroplastic form

weak inhibition

5 mM, 49% residual activity

724568

1.1.3.17

uncompetitive inhibition, 50% inhibition

676916

1.1.3.4

decreases the enzyme activity by about 18% at 2 mM

743193

1.11.1.9

20 mM, 59% residual activity

61% activity at 1 mM

0.2 mM, inhibits the 8R-dioxygenase enzyme by over 85%

285247

1.14.18.1

79.9% residual activity at 5 mM

strong inhibitor

inactivates NPADH, presumably by modifying the active site cysteine

non-competitive

60% residual activity at 1 mM with feruloyl-CoA as substrate

slight inhibition

about 20% inhibition of isoform LAAOII and about 90% inhibition of isoform LAAOI at 5 mM

743013

1.4.3.3

31.8% residual activity at 10 mM

741688

1.5.1.48

0.1 mM, 93% inhibition

i.e. PMSF, chloroplast enzyme, slight inhibition

394774

1.9.6.1

1 mM, 36.8% residual activity

58% inhibition

complete inhibition at 10 mM

complete inhibition at 5 mM

30% inhibition at 50 mM

treatment of purified SlCGT with 1, 10, and 50 mM PMSF leads to strong decreases in caffeoyl transfer activities to 60%, 40%, and 0% activity, respectively

slight inhibition at 1 mM

638140

2.4.1.18

70.88% residual activity at 1 mM

756764

2.4.1.219

strong inhibition at 10 mM

1 mM, 65% irreversible inhibition, 1.5 M ATP protect

inhibits 65% at 5 mM

about 25% residual activity at 1 mM

753524

3.1.1.113

almost complete inhibition

1 mM

1 mM, 54% inhibition

1 mM, 15% of initial activity; 1 mM, 55% of initial activity; 1 mM, 60% of initial activity

50% inhibition at about 8 mM PMSF

75% inhibition at 1 mM

20% inhibition at 1 mM

1 mM, 26.4% inhibition, Phedase type 2; 1 mM, 61% inhibition, Phedase type 1

676327

3.1.1.84

1 mM, complete inhibition

698540

3.1.1.85

10.5% residual activity after 10 min of incubation with 2 mM

652416

3.1.1.86

50 mM, 100% inhibition

strong inhibition of 4-nitrophenylacetate hydrolysis

734779

3.1.2.12

inhibition of wild-type YeiG and YeiG mutants C54A and C26A

FS-44: 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase

208374

3.1.4.12

70.3% residual activity at 2 mM

2 mM, 94% inhibition

21.2% inhibition at 2 mM

5 mM, 60% inhibition

23% residual activity in the presence of 5 mM phenylmethylsulfonyl fluoride, after 30 min at 30°C

about 2% residual activity at 1% (v/v)

78% inhibition at 5 mM

680971

3.2.1.157

47.5% inhibition at 1 mM

no inhibition of isozyme MG4, 47% inhibition of isozyme MG6

679331

3.2.1.203

35.54% residual activity at 10 mM

partially

about 35% residual activity at 1 mM

750933

3.2.1.54

10 mM, activity decreases by about 50%

1 mM, 4°C, 10 h incubation, 76% inhibition

136738

3.2.1.67

81.4% residual activity at 10 mM

750600

3.2.1.68

9% inhibition at 5 mM

732278

3.2.1.78

80.3% residual activity at 5 mM

strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C

5 mM, inhibition to 75% of control

768468

3.2.1.B1

about 60% residual activity at 1 mM; about 65% residual activity at 10 mM

731420

3.3.2.10

27% inhibition at 1 mM at pH 7.4

661511

3.3.2.2

6 mM 29% inhibition; 6 mM, 29% inhibition

1 mM, 40% inhibition; 1 mM, 64% inhibition

70.04% residual activity at 10 mM

41% inhibition at 1 mM

88.5% inhibition at 1 mM

753644

3.4.14.1

less than 40% residual activity at 1 mM

1 mM, no residual activity

weak

0.1 mM and 1 mM, 78.1% and 21.4% compared to a control without additives

95250

3.4.18.1

0.1 mM, proPoCtX: 42.2% inhibition, native PoCtX: 37.2% inhibition

weak

2 mM, 15% inhibition

10 mM, 21% inhibition

649268

3.4.21.106

80% inhibition at 1 mM

51% inhibition at 5 mM

651080

3.4.21.112

12% inhibition at 0.05 mM

36549, 36550, 36551, 36556

3.4.21.35

3 entries

3.4.21.36

36559, 36563, 36571, 36572

1 mM, 42% inhibition of amidolytic activity

652686

3.4.21.48

30710, 95499, 95501

3.4.21.5

2 mM, complete inhibition

29377, 29382, 29408

complete inhibition

partial inhibition

50% loss of activtity

0.6 mM, 50% inhibition

at high concentrations

almost complete inhibition at 10 mM

20 mM

80% ihibition

1 mM, 87% inhibition

5 mM, 21% inhibition

1 mM, 87% inhibition

weak inhibition

53.68% inhibition at 0.1 mM

708216

3.4.22.39

36705, 36706, 36711

3.4.22.41

13.22% inhibition at 0.1 mM

1 mM, 6% inhibition

incubation with 100 mM phenylmethylsulfonyl fluoride for 30 min decreases activity to 63% of the initial value, but inhibition is partially reverted when 12 mM cysteine is added to the reaction mixture

complete inhibition

3 mM, 16% inhibition

PMSF does not affect activity when using 2-(N-methylamino)benzoyl-AGAGIIETk(Dnp) as a substrate. With 2-(N-methylamino)benzoyl-AGAGIIETk(Dnp), PMSF shows no effect on truncated protease and 30% inhibition of full-length NSP2

755412

3.4.23.18

16.3% residual activity at 5 mM

752341

3.4.23.20

complete inhibition at 10 mM

weak inhibitor

slight

weak inhibition

weak

2 mM, 36.4% inhibition

768748

3.4.24.30

strongly inhibited, 100% inhibition

50% inhibition at 1 mM

PMSF, 80% inhibition at 1 mM

1 mM, 68% inhibition after 1 h

slight inhibition

1 mM, 45% inhibition

10 mM: 9.8% activity; strong inhibition

31% inhibition at 1 mM, complete inhibition at 10 mM

2 mM, 10% inhibition

weak

weak

1 mM, 77% loss of activity

strong inhibition, 0.1 mM: 95% inhibition, 0.01 mM: 84% inhibition

1 mM, 80% inhibition

5 mM, 31% inhibition

irreversible inhibition

734439

3.5.1.B18

15% inhibition at 1 mM

683975

3.5.3.1

1 mM, 64% loss of activity

771016

3.5.3.18

NG,NG-dimethyl-L-arginine release from freeze-thaw whole blood 57% inhibited

667159

3.5.3.6

1 mM, dextran-conjugated enzyme and native enzyme retain 1.1% and 1.2% of their initial activities

1 mM, 51% inhibition

about 10% residual activity at 1% (w/v)

1 mM, 71.7% residual activity

10 mM: 63% of maximal activity

83% reduced activity at 1 mM

1 mM, 7.6% inhibition

2 mM, 33% inhibition

10 mM, 59% inhibition

52.3% residual activity at 10 mM

768962

5.1.1.15

59% inhibition at 1 mM

748352

5.3.1.7

partial inhibition at 0.1 mM, complete inhibition at 1 mM

650629

6.2.2.2

1 mM, strong inhibition

756443

6.2.2.3

1 mM, strong inhibition

756443

Metals and Ions (4 results)

activates 12% at 1 mM

749624

3.2.1.8

activation of isozymes A and B

655257

3.4.25.1

activates chymotrypsin-like activity

137351

3D Structure of Enzyme-Ligand-Complex (PDB) (3 results)

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

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1.3.8.6

391409

K_i Value (3 results)

0.15

0.1

0.6

IC₅₀ Value (16 results)

0.2

at pH 7.4 and 37Â°C

0.24

mediocre inhibitor, IC50: 0.24 mM

0.6

IC50: 0.6 mM

650716

3.7.1.17

0.63

pH 7.5, 21°C, recombinant enzyme

733885

References & Links

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Show Reference (1142 entries)

Links to other databases for phenylmethanesulfonyl fluoride

Ligand phenylmethanesulfonyl fluoride

Basic Ligand Information

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (2 results)

Activator in Enzyme-catalyzed Reactions (31 results)

Inhibitor in Enzyme-catalyzed Reactions (917 results)

Metals and Ions (4 results)

3D Structure of Enzyme-Ligand-Complex (PDB) (3 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (1 result)

Ki Value (3 results)

IC50 Value (16 results)

References & Links

Literature References (1142)

Links to other databases for phenylmethanesulfonyl fluoride

k_cat Value (Turnover Number) (1 result)

K_i Value (3 results)

IC₅₀ Value (16 results)