Ligand N-Formyl-Met-Leu-Phe

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Basic Ligand Information

Molecular Structure
Picture of N-Formyl-Met-Leu-Phe (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C21H31N3O5S
N-Formyl-Met-Leu-Phe
SBHXYTNGIZCORC-ZDUSSCGKSA-N
Synonyms:
fMLP, formyl-Met-Leu-Phe, formyl-methionine-leucine-phenylalanine, formyl-methionyl-leucyl-phenylalanine, formyl-methionyl-leucylphenylalanine, formylmethionine-Leu-Phe, N-formyl-L-Met-Leu-Phe, N-formyl-L-methionyl-L-leucyl-L-phenylalanine, N-formyl-Met-Leu-Phe(fMLP), N-formyl-methionyl-leucyl-phenylalanine, N-formylmethionyl-leucyl-phenylalanine

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
N-formyl-Met-Leu-Phe + H2O = ?
show the reaction diagram
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N-formyl-Met-Leu-Phe(fMLP) + H2O = N-formyl-Met + Leu-Phe(fMLP)
show the reaction diagram
-
N-formyl-L-Met-Leu-Phe + H2O = ?
show the reaction diagram
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ATP + H2O + formyl-methionyl-leucine-phenylalanine/in = ADP + phosphate + formyl-methionyl-leucine-phenylalanine/out
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ATP + H2O + formyl-methionyl-leucine-phenylalanine/in = ADP + phosphate + formyl-methionyl-leucine-phenylalanine/out
show the reaction diagram
-
-

Substrate in Enzyme-catalyzed Reactions (8 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
N-formyl-Met-Leu-Phe + H2O = ?
show the reaction diagram
-
N-formyl-Met-Leu-Phe(fMLP) + H2O = N-formyl-Met + Leu-Phe(fMLP)
show the reaction diagram
-
formylmethionine-Leu-Phe + H2O = ?
show the reaction diagram
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N-Formyl-Met-Leu-Phe + H2O = N-Formyl-Met + Leu-Phe
show the reaction diagram
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N-formyl-Met-Leu-Phe + H2O = formate + Met-Leu-Phe
show the reaction diagram
-
ATP + H2O + formyl-methionyl-leucine-phenylalanine/in = ADP + phosphate + formyl-methionyl-leucine-phenylalanine/out
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ATP + H2O + formyl-methionyl-leucine-phenylalanine/in = ADP + phosphate + formyl-methionyl-leucine-phenylalanine/out
show the reaction diagram
-
-

Activator in Enzyme-catalyzed Reactions (13 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
stimulation of carboxyl methylation of GTP-binding proteins through a substrate-dependent mechanism
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stimulation is dependent on the preincubation of cells with cytochalasin B, addition of tumor necrosis factor increases 50% alkyl-2-acetyl-sn-glycero-3-phosphocholine production
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induces release of enzyme from neutrophils
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a chemotactic peptide, stimulates the enzyme and its release from primary granules of PMN
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treatment of whole blood leads to stimulation of neutrophils resulting in activation of the alternative complement pathway and release of C5 fragments, which further amplify proinflammatory responses
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PR3 and CD177 are increased in parallel. Causes a moderate increase. stimulating cells with cytochalasin B in combination with fML, PmPR3- and CD177-negative cells become positive
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Ca2+ is required for the N-formyl-Met-Leu-Phe-induced and the A23187-induced stimulation
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (3 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
12.3
-
-
12.3
-
-
0.7
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enzyme form PDF1A

KM Value (3 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.18
-
-
0.18
-
-
0.23
-
enzyme form PDF1A

References & Links

Links to other databases for N-Formyl-Met-Leu-Phe

ChEBI
PubChem
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PubChem