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Ligand L-2-amino-3-oxobutanoate

Basic Ligand Information

Molecular Structure

Related pathways

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Roles as Enzyme Ligand

In Vivo Product in Enzyme-catalyzed Reactions (3 results)

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Show Natural Product (3 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.103

L-threonine + NAD+ = (2S)-2-amino-3-oxobutanoate + NADH + H+

285720, 285707, 285708, 285713, 285705, 285721, 285719, 285715, 285717, 285701, 285703, 285704, 285706, 285709, 285710, 285711, 285712, 285714, 285716, 285718, 285702, -

-

1.1.1.103

L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH + H+

-

-

1.1.1.103

2 entries

2.3.1.29

acetyl-CoA + glycine = CoA + L-2-amino-3-oxobutanoate

-

1FC4, 3D-view

Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Substrate (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.1.29

CoA + L-2-amino-3-oxobutanoate = acetyl-CoA + glycine

777099

1FC4, 3D-view

Product in Enzyme-catalyzed Reactions (9 results)

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Show Product (9 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.103

L-threonine + NAD+ = (2S)-2-amino-3-oxobutanoate + NADH + H+

285720, 285707, 285708, 285713, 285705, 285721, 285719, 285715, 285717, 285701, 285703, 285704, 285706, 285709, 285710, 285711, 285712, 285714, 285716, 285718, 285702, -

-

1.1.1.103

L-allothreonine + NAD+ = L-2-amino-3-oxobutanoate + NADH

285713

-

1.1.1.103

L-threonine + 3-acetyl-pyridine adenine dinucleotide = L-2-amino-3-oxobutanoate + ?

-

-

1.1.1.103

L-threonine + 3-pyridinealdehyde adenine dinucleotide = L-2-amino-3-oxobutanoate + ?

-

-

1.1.1.103

L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH + H+

-

-

1.1.1.103

L-threonine + nicotinamide guanine dinucleotide = L-2-amino-3-oxobutanoate + ?

-

-

1.1.1.103

L-threonine + thionicotinamide-NAD+ = L-2-amino-3-oxobutanoate + ?

-

-

1.1.1.103

7 entries

1.1.1.276

L-allo-threonine + NADP+ = L-2-amino-3-ketobutyrate + NADPH

639084

-

2.3.1.29

acetyl-CoA + glycine = CoA + L-2-amino-3-oxobutanoate

-

1FC4, 3D-view

Inhibitor in Enzyme-catalyzed Reactions (1 result)

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Show Inhibitors (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.103

competitive product inhibition by the unstable L-2-amino-3-oxobutyrate only in presence of NADH, which stabilizes

668536

-

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (1 entry)

EC NUMBER

ENZYME 3D STRUCTURE

2.3.1.29

1FC4, 3D-view

Enzyme Kinetic Parameters

References & Links

Literature References (24)

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Show Reference (24 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

285701

11470445

Characterization of hepatic L-threonine dehydrogenase of chicken

2001

Yuan, J.H.; austic, R.E.

Comp. Biochem. Physiol. B

130

65-73

285702

9784233

Investigation of a catalytic zinc binding site in Escherichia coli L-threonine dehydrogenase by site-directed mutagenesis of cysteine-38

1998

Johnson, A.R.; Chen, Y.W.; Dekker, E.E.

Arch. Biochem. Biophys.

358

211-221

285703

7840645

Identification of a second active site residue in Escherichia coli L-threonine dehydrogenase: methylation of histidin-90 with methyl p-nitrobenzenesulfonate

1995

Marcus, J.P.; Dekker, E.E.

Arch. Biochem. Biophys.

316

413-420

285704

8519804

Functional analysis of E. coli threonine dehydrogenase by means of mutant isolation and characterization

1995

Chen, Y.W.; Dekker, E.E.; Somerville, R.L.

Biochim. Biophys. Acta

1253

208-214

285705

7827500

Purification and structural characterization of porcine L-threonine dehydrogenase

1994

Kao, Y.C.; Davis, L.

Protein Expr. Purif.

5

423-431

285706

8407827

Threonine formation via the coupled activity of 2-amino-3-ketobutyrate coenzyme A lyase and threonine dehydrogenase

1993

Marcus, J.P.; Dekker, E.E.

J. Bacteriol.

175

6505-6511

285707

-

Mitochondrial L-threonine dehydrogenase

1992

Pagani, R.; Leoncini, R.; Righi, S.; Guerranti, R.; Lazzerretti, L.; Marinello, E.

Biochem. Soc. Trans.

20

379

285708

-

Rat liver L-threonine dehydrogenase

1991

Pagani, R.; Guerranti, R.; Right, S.; Leoncini, R.; Vannoni, D.; Marinello, E.

Biochem. Soc. Trans.

20

24

285709

2007567

L-Threonine dehydrogenase from Escherichia coli

1991

Epperly, B.R.; Dekker, E.E.

J. Biol. Chem.

266

6086-6092

285710

2104757

The sulfhydryl content of L-threonine dehydrogenase from Escherichia coli K-12: relation to catalytic activity and Mn2+ activation

1990

Craig, P.A.; Dekker, E.E.

Biochim. Biophys. Acta

1037

30-38

285711

2647748

The primary structure of Escherichia coli L-threonine dehydrogenase

1989

Aronson, B.D.; Somerville, R.L.; Epperly, B.R.; Dekker, E.E.

J. Biol. Chem.

264

5226-5232

285712

3056527

Cd2+ activation of L-threonine dehydrogenase from Escherichia coli K-12

1988

Craig, P.A.; Dekker, E.E.

Biochim. Biophys. Acta

957

222-229

285713

3536927

Interaction between L-threonine dehydrogenase and aminoacetone synthetase and mechanism of aminoacetone production

1986

Tressel, T.; Thompson, R.; Zieske, L.R.; Menendez, M.I.T.S.; Davis, L.

J. Biol. Chem.

261

16428-16437

285714

3518793

L-Threonine dehydrogenase from Escherichia coli K-12: thiol-dependent activation by Mn2+

1986

Craig, P.A.; Dekker, E.E.

Biochemistry

25

1870-1876

285715

3888978

L-Threonine dehydrogenase from goat liver

1985

Ray, M.; Ray, S.

J. Biol. Chem.

260

5913-5918

285716

6780553

L-threonine dehydrogenase

1981

Boylan, S.A.; Dekker, E.E.

J. Biol. Chem.

256

1809-1815

285717

7028754

L-Threonine dehydrogenase of chicken liver

1981

Aoyama, Y.; Motokawa, Y.

J. Biol. Chem.

256

12367-12373

285718

33672

L-Threonine dehydrogenase of Escherichia coli K-12

1978

Boylan, S.A.; Dekker, E.E.

Biochem. Biophys. Res. Commun.

85

190-197

285719

-

L-Threonine dehydrogenase (Arthrobacter)

1971

McGilvray, D.; Morris, J.G.

Methods Enzymol.

17B

580-584

285720

4284408

The enzymatic formation of aminoacetone from threonine and its further metabolism

1964

Green, M.L.; Elliott, W.H.

biochem. J.

92

537-548

285721

14165492

Studies on liver threonine dehydrogenase

1964

Hartshorne, D.; Greenberg, D.M.

Arch. Biochem. Biophys.

105

173-178

639084

12535615

Characterization of short-chain dehydrogenase/reductase homologues of Escherichia coli (YdfG) and Saccharomyces cerevisiae (YMR226C)

2003

Fujisawa, H.; Nagata, S.; Misono, H.

Biochim. Biophys. Acta

1645

89-94

668536

15902509

L-Threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3: gene cloning and enzymatic characterization

2005

Shimizu, Y.; Sakuraba, H.; Kawakami, R.; Goda, S.; Kawarabayasi, Y.; Ohshima, T.

Extremophiles

9

317-324

777099

36697628

Chemoenzymatic synthesis of 3-ethyl-2,5-dimethylpyrazine by L-threonine 3-dehydrogenase and 2-amino-3-ketobutyrate CoA ligase/L-threonine aldolase

2021

Motoyama, T.; Nakano, S.; Hasebe, F.; Miyata, R.; Kumazawa, S.; Miyoshi, N.; Ito, S.

Commun. Chem.

4

108

Links to other databases for L-2-amino-3-oxobutanoate

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