Ligand L-Leu-4-nitroanilide

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Basic Ligand Information

Molecular Structure
Picture of L-Leu-4-nitroanilide (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C12H17N3O3
L-Leu-4-nitroanilide
AXZJHDNQDSVIDR-NSHDSACASA-N
Synonyms:
L-Leu-p-nitroanilide, L-Leu 4-nitroanilide, L-leucine-4-nitroanilide, L-leucine-p-nitroanilide, L-leucine 4-nitroanilide, L-leucine p-nitroanilide, L-leucyl-4-nitroanilide, L-leucyl 4-nitroanilide, L-Leu p-nitroanilide, Leu-4-nitroanilide, Leu-p-nitroanilide, Leu-para-nitroanilide, Leu 4-nitroanilide, leucine-4-nitroaniline, leucine p-nitroanilide, leucyl-p-nitroanilide, N-(4-nitrophenyl)-L-leucinamide

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (66 results)

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EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
L-leucine-4-nitroanilide + H2O = L-leucine + 4-nitroaniline
show the reaction diagram
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Leu-4-nitroanilide + H2O = Leu + 4-nitroaniline
show the reaction diagram
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Leu-p-nitroanilide + H2O = Leu + p-nitroaniline
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Leu-p-nitroanilide + H2O = L-leucine + p-nitroaniline
show the reaction diagram
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Leu-4-nitroanilide + H2O = Leu + 4-nitroaniline
show the reaction diagram
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L-leucyl 4-nitroanilide + H2O = L-leucine + 4-nitroaniline
show the reaction diagram
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L-leucyl 4-nitroanilide + H2O = L-leucine + 4-nitroaniline
show the reaction diagram
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L-leucyl 4-nitroanilide + H2O = L-leucine + 4-nitroaniline
show the reaction diagram
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L-leucyl 4-nitroanilide + H2O = L-leucine + 4-nitroaniline
show the reaction diagram
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Leu-4-nitroanilide + H2O = Leu + 4-nitroaniline
show the reaction diagram
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L-leucyl-4-nitroanilide + H2O = L-leucine + 4-nitroaniline
show the reaction diagram
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L-Leu-4-nitroanilide + H2O = L-Leu + 4-nitroaniline
show the reaction diagram
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Leu-p-nitroanilide + H2O = Leu + 4-nitroaniline
show the reaction diagram
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L-leucine-4-nitroanilide + H2O = L-leucine + 4-nitroaniline
show the reaction diagram
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L-Leu p-nitroanilide + H2O = Leu + p-nitroaniline
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Product in Enzyme-catalyzed Reactions (2 results)

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EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
Benzyloxycarbonyl-Ala-Ala-Leu 4-nitroanilide + H2O = Benzyloxycarbonyl-Ala-Ala + Leu 4-nitroanilide
show the reaction diagram
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succinyl-Gly-Gly-Leu-4-nitroanilide + H2O = Leu-4-nitroanilide + succinyl-Gly-Gly
show the reaction diagram
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Inhibitor in Enzyme-catalyzed Reactions (1 result)

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EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (121 results)

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EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
4.2
-
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1.65
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pH 7.2, 37°C
340
-
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749.7
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at pH 8.0 and 37°C

KM Value (159 results)

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EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.5
3
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0.25
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with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C
3.39
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0.1
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pH 7.0, 70°C
0.093
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at pH 8.0 and 37°C
12.5
-
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0.2
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Ki Value (1 result)

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EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0055
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enzyme with 2 Co2+ or Zn2+ bound, pH 8.0, 25°C

References & Links

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Links to other databases for L-Leu-4-nitroanilide

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ChEBI
PubChem
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PubChem