Ligand gentiobiose

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Basic Ligand Information

Molecular Structure
Picture of gentiobiose (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C12H22O11
gentiobiose
DLRVVLDZNNYCBX-LIZSDCNHSA-N
Synonyms:
6-O-(beta-D-glucopyranosido)-beta-D-glucopyranoside, 6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside, 6-O-beta-D-glucopyranosyl-D-glucopyranoside, beta-D-Glc-(1->6)-beta-D-Glc, beta-gentiobiose, D-gentiobiose, D-gentobiose, gentobiose
Pathway Source
Pathways

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ATP + gentiobiose = ADP + 6-phospho-beta-D-glucosyl-(1,6)-D-glucose
show the reaction diagram
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gentiobiose + H2O = 2 beta-D-glucose
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

Substrate in Enzyme-catalyzed Reactions (40 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
gentiobiose + NAD+ = ? + NADH
show the reaction diagram
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gentiobiose + ferricenium hexafluorophosphate = ?
show the reaction diagram
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sucrose + gentiobiose = ?
show the reaction diagram
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gentiobiose + starch = ?
show the reaction diagram
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UDPgalactose + gentiobiose = ?
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gentiobiose + alpha-D-mannose 1-phosphate = ? + phosphate
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gentiobiose + alpha-D-glucose 1-phosphate = 1,3-beta-D-glucosylgentiobiose + phosphate
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sucrose + gentobiose = ?
show the reaction diagram
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beta-D-glucose-1-phosphate + gentiobiose = 6-O-beta-D-glucopyranosyl trehalose + phosphate
show the reaction diagram
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ATP + gentiobiose = ADP + 6-phospho-beta-D-glucosyl-(1,6)-D-glucose
show the reaction diagram
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phosphatidylglycerol + beta-gentiobiose = sn-1,2-diacylglycerol + beta-gentiobiose-6-phosphoglycerol
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D-gentiobiose = ?
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gentiobiose + H2O = 2 beta-D-glucose
show the reaction diagram
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gentiobiose + H2O = D-glucose
show the reaction diagram
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gentiobiose + H2O = D-glucopyranose
show the reaction diagram
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gentiobiose + phenyl alpha-D-galactoside = ?
show the reaction diagram
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gentiobiose + H2O = 2 beta-D-glucose
show the reaction diagram
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gentiobiose + H2O = ?
show the reaction diagram
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6-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O = ?
show the reaction diagram
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gentiobiose + H2O = ?
show the reaction diagram
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gentiobiose + H2O = D-glucose
show the reaction diagram
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Asn-(GlcNAc)2(Man)6 + gentiobiose = ?
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gentiobiose + H2O = 2 beta-D-glucose
show the reaction diagram
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gentiobiose + H2O = D-glucose + D-glucose
show the reaction diagram
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beta-D-Glc-(1->6)-beta-D-Glc + H2O = beta-D-glucose
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (28 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
p-nitrophenyl beta-gentiobioside + H2O = 4-nitrophenol + beta-gentiobiose
show the reaction diagram
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laminaran + H2O = D-glucose + gentiobiose + gentiotriose
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
stimulate
-

Inhibitor in Enzyme-catalyzed Reactions (10 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
1 mM, 22% inhibition of reaction with lactose, 0.00625 mM
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a non-competitive inhibitor
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at 1-5 mM, competitive to membrane-derived oligosaccharides
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5 mM, competitive to membrane-derived oligosaccharides, 15% of the activity without addition
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slight inhibition of acid trehalase
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20 mM, uncompetitive inhibition with starch as substrate
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Metals and Ions (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activates the neutral trehalase
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (16 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
26.1
-
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.056
-
pH 5.0, 50°C

KM Value (34 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
17
-
at 30°C with 0.4 mM ferricenium as electron acceptor
60.8
-
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
1
-
pH 7.8, 37°C
22
-
-
10.7
-
-

Ki Value (4 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

IC50 Value (1 result)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.05
-
pH and temperature not specified in the publication

References & Links

Links to other databases for gentiobiose

ChEBI
PubChem
ChEBI
PubChem