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Ligand (-)-vetispiradiene

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Product in Enzyme-catalyzed Reactions (1 result)

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Show Natural Product (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

4.2.3.21

trans,trans-farnesyl diphosphate = vetispiradiene + diphosphate

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Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Substrate (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.14.14.151

(-)-vetispiradiene + [reduced NADPH-hemoprotein reductase] + O2 = solavetivol + [oxidized NADPH-hemoprotein reductase] + H2O

712360

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Product in Enzyme-catalyzed Reactions (1 result)

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Show Product (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

4.2.3.21

trans,trans-farnesyl diphosphate = vetispiradiene + diphosphate

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Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (4 results)

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Show Turnover Number (4 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.14.14.151

2.1

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wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30°C

712360

1.14.14.151

3.8

-

mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30°C

712360

1.14.14.151

12.7

-

mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C

712360

1.14.14.151

20.7

-

mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C

712360

1.14.14.151

4 entries

K_M Value (4 results)

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Show KM Value (4 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.14.14.151

0.0062

-

mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30°C

712360

1.14.14.151

0.0084

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mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C

712360

1.14.14.151

0.013

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mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C

712360

1.14.14.151

0.014

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wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30°C

712360

1.14.14.151

4 entries

References & Links

Literature References (1)

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Show Reference (1 entry)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

712360

17715131

Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates

2007

Takahashi, S.; Yeo, Y.S.; Zhao, Y.; O'Maille, P.E.; Greenhagen, B.T.; Noel, J.P.; Coates, R.M.; Chappell, J.

J. Biol. Chem.

282

31744-31754

Links to other databases for (-)-vetispiradiene

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Show Links (1 entry)

ChEBI

PubChem

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