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Ligand 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose

Basic Ligand Information

Molecular Structure

Show all BRENDA pathways known for 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Natural Substrate (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.114

3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O = alpha-D-mannopyranose

710037

-

3.2.1.24

alpha-(1-3)-mannobiose + H2O = alpha-D-mannopyranose + D-mannopyranose

135965

-

Substrate in Enzyme-catalyzed Reactions (12 results)

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Show Substrate (12 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.101

UDP-N-acetyl-D-glucosamine + Manalpha(1-3)Man = UDP + ?

658192, 659000

-

2.4.2.38

UDP-D-xylose + D-mannosyl-alpha-(1,3)-D-mannose = UDP + D-xylosyl-beta-(1,2)-D-mannosyl-alpha-(1,3)-D-mannose

692993, 693051

-

2.7.8.32

UDP-xylose + 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose = UMP + 3-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose

704602

-

3.2.1.114

3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O = 2 alpha-D-mannopyranose

710037

-

3.2.1.114

3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O = alpha-D-mannopyranose

710037, 710392

-

3.2.1.114

alpha-D-Manp-(1,3)-D-Manp + H2O = alpha-D-mannopyranose

664527

-

3.2.1.24

1,3-alpha-D-mannobiose + H2O = alpha-D-mannose

680469

-

3.2.1.24

3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O = alpha-D-mannopyranose

710392

-

3.2.1.24

alpha-(1-3)-mannobiose + H2O = alpha-D-mannopyranose + D-mannopyranose

135965

-

3.2.1.24

Man-alpha(1-3)Man + H2O = alpha-D-mannose

698317

-

3.2.1.24

Manalpha(1-3)Man + H2O = D-mannose

698317

-

3.2.1.24

Manalpha1-3Man + H2O = alpha-mannose

656917

-

3.2.1.114

3 entries

3.2.1.24

6 entries

Product in Enzyme-catalyzed Reactions (4 results)

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Show Product (4 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.130

methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranoside + H2O = alpha-(1->3)-D-mannobiose + methyl alpha-(1->2)-D-mannobioside + ?

0

-

3.2.1.198

4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O = 4-methylumbelliferone + (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside

0

-

3.2.1.198

methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside + H2O = alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside

0

-

3.2.1.198

methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranoside + H2O = alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside

0

-

3.2.1.198

3 entries

Inhibitor in Enzyme-catalyzed Reactions (2 results)

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Show Inhibitors (2 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.114

in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose

646575

-

3.2.1.24

50% inhibition at 18 mM

136002

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (3 results)

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Show Turnover Number (3 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.2.1.114

4.67

-

pH 7.0, 37°C, Bt3991

710037

3.2.1.114

6.4

-

pH 6.8, 37°C, recombinant enzyme

710392

3.2.1.114

2 entries

3.2.1.24

6.4

-

pH 6.8, 37°C, recombinant enzyme

710392

K_M Value (5 results)

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Show KM Value (5 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.2.1.114

9

-

pH 7.0, 37°C, Bt3991

710037

3.2.1.114

27

-

pH 6.8, 37°C, recombinant enzyme

710392

3.2.1.24

0.839

-

pH 6.5, 50°C, 20 min

698317

3.2.1.24

0.839

-

pH 6.5, 50°C

698317

3.2.1.24

27

-

pH 6.8, 37°C, recombinant enzyme

710392

3.2.1.114

2 entries

3.2.1.24

3 entries

References & Links

Literature References (14)

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Show Reference (14 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

135965

7632718

Purification and characterization of alpha-D-mannosidase from Aspergillus sp.

1995

Gaikwald, S.M.; Keskar, S.S.; Khan, M.I.

Biochim. Biophys. Acta

1250

144-148

136002

7240229

Purification and characterization of a phospholipid-dependent alpha-mannosidase from rabbit liver

1981

Forsee, W.T.; Schutzbach, J.S.

J. Biol. Chem.

256

6577-6582

646575

4051507

Characterization of microsomal and cytosolic alpha-1,2-mannosidases from mung bean hypocotyls

1985

Forsee, W.T.

Arch. Biochem. Biophys.

242

48-57

656917

11319010

Rice alpha-mannosidase digesting the high mannose glycopeptide of glutelin

2001

Kishimoto, T.; Hori, H.; Takano, D.; Nakano, Y.; Watanabe, M.; Mitsui, T.

Physiol. Plant.

112

15-24

658192

12878032

Are there two forms of beta 2-N-acetylglucosaminyltransferase I in rat testicular and epididymal fluids?

2003

Nozaki, H.; Matsuzawa, T.; Nakamura, T.; Arai, I.; Urashima, T.

Biochim. Biophys. Acta

1649

140-145

659000

-

N-Acetylglucosaminyltransferase I activity in bovine ovarian follicular fluids from dominant and atretic follicles

2004

Nozaki, H.; Miyamoto, A.; Hayashi, K.G.; Matsui, M.; Yoshida, T.; Nakamura, T.; Arai, I.; Urashima, T.

J. Appl. Glycosci.

51

315-320

664527

15721331

The production, purification and characterization of two novel alpha-D-mannosidases from Aspergillus phoenicis

2005

Athanasopoulos, V.I.; Niranjan, K.; Rastall, R.A.

Carbohydr. Res.

340

609-617

680469

17015651

Molecular and biochemical characterization of alpha-glucosidase and alpha-mannosidase and their clustered genes from the thermoacidophilic archaeon Picrophilus torridus

2006

Angelov, A.; Putyrski, M.; Liebl, W.

J. Bacteriol.

188

7123-7131

692993

17430900

A beta-1,2-xylosyltransferase from Cryptococcus neoformans defines a new family of glycosyltransferases

2007

Klutts, J.S.; Levery, S.B.; Doering, T.L.

J. Biol. Chem.

282

17890-17899

693051

18347023

Cryptococcal xylosyltransferase 1 (Cxt1p) from Cryptococcus neoformans plays a direct role in the synthesis of capsule polysaccharides

2008

Klutts, J.S.; Doering, T.L.

J. Biol. Chem.

283

14327-14334

698317

19038284

Class II alpha-mannosidase from Aspergillus fischeri: energetics of catalysis and inhibition

2009

Shashidhara, K.S.; Gaikwad, S.M.

Int. J. Biol. Macromol.

44

112-115

704602

19864415

A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans

2009

Reilly, M.C.; Levery, S.B.; Castle, S.A.; Klutts, J.S.; Doering, T.L.

J. Biol. Chem.

284

36118-36127

710037

20081828

Mechanistic insights into a Ca2+-dependent family of alpha-mannosidases in a human gut symbiont

2010

Zhu, Y.; Suits, M.D.; Thompson, A.J.; Chavan, S.; Dinev, Z.; Dumon, C.; Smith, N.; Moremen, K.W.; Xiang, Y.; Siriwardena, A.; Williams, S.J.; Gilbert, H.J.; Davies, G.J.

Nat. Chem. Biol.

6

125-132

710392

20140249

Structure and kinetic investigation of Streptococcus pyogenes family GH38 alpha-mannosidase

2010

Suits, M.D.; Zhu, Y.; Taylor, E.J.; Walton, J.; Zechel, D.L.; Gilbert, H.J.; Davies, G.J.

PLoS ONE

5

e9006

Links to other databases for 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose

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Show Links (1 entry)

ChEBI

PubChem