Ligand 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide

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Basic Ligand Information

Molecular Structure
Picture of 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C18H17BrN5S
2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide
AZKSAVLVSZKNRD-UHFFFAOYSA-M
Synonyms:
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, methylthiazolyltetrazolium bromide, oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, reduced 3(4,5-dimethyl thiazolyl 1-2) 2,5-diphenyl tetrazolium bromide, reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, thiazolyl blue

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (14 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
(R)-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide = pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
show the reaction diagram
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6-hydroxynicotinate + H2O + thiazolyl blue = 2,6-dihydroxynicotinate + H2O2 + reduced thiazolyl blue
show the reaction diagram
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succinate + 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide = ?
show the reaction diagram
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3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide + H2O = ?
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
D-proline + oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide = DELTA1-pyrroline 2-carboxylate + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
show the reaction diagram
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NADPH + H+ + oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide = NADP+ + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
show the reaction diagram
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3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide + NAD(P)H = reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide + NAD(P)+
show the reaction diagram
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (9 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE

KM Value (1 result)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.247
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pH 8.5, 60C, cosubstrate: (R)-lactate

References & Links

Links to other databases for 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide

ChEBI
PubChem
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PubChem