Ligand phosphatidylserine

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Basic Ligand Information

Molecular Structure
Picture of phosphatidylserine (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C14H24NO10PR2
phosphatidylserine
IIEROLVPXPBRRZ-QWRGUYRKSA-N
Synonyms:
(3-sn-phosphatidyl)-L-serine, 1,2-diacyl-sn-glycero-3-phospho-L-serine, 1,2-diacyl-sn-glycerol-3-phospho-L-serine, 3-O-sn-phosphatidyl-L-serine, L-1-phosphatidylserine, O3-Phosphatidyl-L-serine, phosphatidyl-L-serine

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
phosphatidylserine + H2O = serine + phosphatidic acid
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (8 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

Substrate in Enzyme-catalyzed Reactions (23 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
phosphatidylserine + NADH + H+ + O2 = ?
show the reaction diagram
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phosphatidylserine + H2O = ?
show the reaction diagram
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phosphatidylserine + H2O = ?
show the reaction diagram
-
phosphatidylserine + H2O = phosphorylserine + diacylglycerol
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (15 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
lyso-Phosphatidylserine + phospholipid = Phosphatidylserine + lyso-phospholipid
show the reaction diagram
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CDP-1,2-diacylglycerol + L-serine = CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (84 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
4fold increase in activity
-
7.2fold activation of horse heart cytochrome c mediated reaction, 4.7fold activation of Candida krusei cytchrome c mediated reaction
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activation of mitochondrial enzyme
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incorporation in membrane elevates acyltransferase activity
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activation, 16-43% as effective as phosphatidylethanolamine
-
33% increase of activity
-
relative activity: 342%
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activation
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enhancement factor: 0.05 in absence of Triton X-100, 0.4 in presence of 0.1% Triton X-100
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activation, addition leads to 100% phosphorylation of tau2-protein
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increases activity
-
solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine
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slight activation, 0.2 mM
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highly activating
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1fold stimulation of amphiphilic enzyme form, 3fold stimulation of hydrophilic enzyme form
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vesicles of phosphatidylserine (PtdSer) greatly stimulate SHIP2. This effect is specific for di-C8 and di-C16 fatty acids of D-myo-phosphatidylinositol 3,4,5-trisphosphate as substrate
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myotubularin-related protein 6 activity increases 1.8fold in both 50% and 100% phosphatidylserine
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allosteric activation
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stimulates
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up-regulates the catalytic activity of the enzyme
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as phospholipid vesicles with 80% phosphatidylcholine and 20% phosphatidylserine, specific binding to the enzyme of anionic phospholipid vesicles passed over the protein C surface, defective phospholipid binding of mutant variants also resulted in severely impaired anticoagulant activity in protein C-deficient plasma
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stimulates
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binding to nonsubstrate anionic interfaces enhances the catalytic activity of PI-PLC, interfacial binding studies, activation mechanism
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mixture of 0.2 mg/ml phosphatidylserine and phosphatidylcholine leads to an 2.4fold increase in dynein activity
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slight stimulation, acyl-CoA synthetase II
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Inhibitor in Enzyme-catalyzed Reactions (44 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
IC50: 0.0013 mM, interaction with acidic phospholipids is most efficient at pH values below pH 6.5
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assumed to be a simple non-competitive inhibition
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inhibition of microsomal enzyme
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above 1.5 mM
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2 mM, 88% inhibition
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0.1 mM, 125% stimulation
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2 mM, 46% inhibition
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negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction
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phosphatidic acid is the most effective inhibitor among acidic phospholipids, followed by phosphatidylserine and phosphatidylinositol, phosphorylated myosin as substrate
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0.2 mM, 75% loss of activity
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strong
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addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 80% of the control activity
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ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids
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Metals and Ions (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
best in lipid vesicles with up to 25% phosphatidyl-L-serine and at least 75% phosphocholine, absolutely required for activity as part of the prothrombinase complex
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Enzyme Kinetic Parameters

KM Value (10 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
2
3
-

Ki Value (3 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0009
0.061
-
0.055
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cytosolic isozyme, pH 7.2, 25°C
0.001
-
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IC50 Value (1 result)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.0013
-
IC50: 0.0013 mM, interaction with acidic phospholipids is most efficient at pH values below pH 6.5

References & Links

Links to other databases for phosphatidylserine

ChEBI
PubChem
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PubChem