Ligand GFL
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Basic Ligand Information
Molecular Structure
C17H25N3O4
GFL
JPVGHHQGKPQYIL-KBPBESRZSA-N
Gly-L-Phe-L-Leu, Gly-Phe-Leu, H-Gly-Phe-Leu-OH
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (2 results)
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Gly-Phe-Leu + H2O = Phe-Leu + Gly-Phe + Gly + Leu
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H-Gly-Phe-Leu-OH + H2O = ?
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Product in Enzyme-catalyzed Reactions (4 results)
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Leu-enkephalin + H2O = Tyr-Gly + Gly-Phe-Leu
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Leu-enkephalin + H2O = Tyr-Gly + Gly-Phe-Leu
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Tyr-Gly-Gly-Phe-Leu + H2O = Tyr-Gly + Gly-Phe-Leu
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Tyr-Gly-Gly-Phe-Leu + H2O = Tyr-Gly + Gly-Phe-Leu
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Inhibitor in Enzyme-catalyzed Reactions (1 result)
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non-competitive inhibitor, 82% inhibition at 1 mM
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (4 results)
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0.006
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pH 8.0, 37°C, 5 M glycerol, product GF
0.013
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pH 8.0, 37°C, product GF
0.026
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pH 8.0, 37°C, 5 M glycerol, product FL
0.045
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pH 8.0, 37°C, product FL
KM Value (4 results)
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0.4
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pH 8.0, 37°C, product FL
0.7
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pH 8.0, 37°C, 5 M glycerol, product GL
1.8
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pH 8.0, 37°C, 5 M glycerol, product FL
2.5
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pH 8.0, 37°C, 5 M glycerol, product GF
Ki Value (1 result)
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0.015
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in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C
References & Links
Literature References (6)
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Dipeptidyl peptidase I from goat brain: purification, characterization and its action on Leu-enkephalin
1993
Pal, S.; Raghav, N.; Kamboj, R.C.; Singh, H.
Neurochem. Int.
22
59-68
Human placental dipeptidyl aminopeptidase III: Hydrolysis of enkephalins and its stimulation by cobaltus ions
1988
Shimamori, Y.; Watanabe, Y.; Fujimoto, Y.
Biochem. Med. Metab. Biol.
40
305-310
Dipeptidyl-aminopeptidase III of rat brain. Selective affinity for enkephalin and angiotensin
1982
Lee, C.M.; Snyder, S.H.
J. Biol. Chem.
257
12043-12050
Rat brain cathepsin L: characterization and differentiation from cathepsin B utilizing opioid peptides
1987
Marks, N.; Berg, M.J.
Arch. Biochem. Biophys.
259
131-143
Additional data about thermolysin specificity in buffer- and glycerol-containing media
1997
Ligne, T.; Pauthe, E.; Monti, J.P.; Gacel, G.; Larreta-Garde, V.
Biochim. Biophys. Acta
1337
143-148
Functional characterization and specific effects of various peptides on enzymatic activity of a DPP-III homologue from goat brain
2008
Dhanda, S.; Singh, H.; Singh, J.; Singh, T.P.
J. Enzyme Inhib. Med. Chem.
23
174-181
Links to other databases for GFL
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