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Ligand deacetylcephalosporin C

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.1.175

acetyl-CoA + deacetylcephalosporin C = CoA + cephalosporin C

673028, 688409, 657712

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In Vivo Product in Enzyme-catalyzed Reactions (4 results)

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Show Natural Product (4 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.14.11.26

deacetoxycephalosporin C + 2-oxoglutarate + O2 = deacetylcephalosporin C + succinate + CO2

0

-

1.14.20.1

deacetoxycephalosporin C + 2-oxoglutarate + O2 = deacetylcephalosporin C + succinate + CO2

0

-

1.14.20.1

deacetoxycephalosporin C + 2-oxoglutarate + O2 = deacetylcephalosporin C + succinate + CO2 + H2O

0

-

1.14.20.1

2 entries

3.1.1.41

cephalosporin C + H2O = deacetylcephalosporin C + acetate

0

-

Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Substrate (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.1.175

acetyl-CoA + deacetylcephalosporin C = CoA + cephalosporin C

657617, 657712, 657802, 658291, 659547, 673028, 688409

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Product in Enzyme-catalyzed Reactions (9 results)

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Show Product (9 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.14.11.26

3-exomethylenecephalosporin C + 2-oxoglutarate + O2 = deacetylcephalosporin C + succinate + CO2 + H2O

0

-

1.14.11.26

deacetoxycephalosporin C + 2-oxoglutarate + O2 = deacetylcephalosporin C + succinate + CO2

0

-

1.14.11.26

deacetoxycephalosporin C + 2-oxoglutarate + O2 = deacetylcephalosporin C + succinate + CO2 + H2O

0

-

1.14.20.1

3-exomethylenecephalosporin C + 2-oxoglutarate + O2 = deacetylcephalosporin C + succinate + CO2 + H2O

440380

-

1.14.20.1

deacetoxycephalosporin C + 2-oxoglutarate + O2 = deacetylcephalosporin C + succinate + CO2

0

-

1.14.20.1

deacetoxycephalosporin C + 2-oxoglutarate + O2 = deacetylcephalosporin C + succinate + CO2 + H2O

0

-

1.14.11.26

3 entries

1.14.20.1

3 entries

3.1.1.41

cephalosporin C + H2O = deacetylcephalosporin C + acetate

0, 170672, 651823, 649397, 170669, 170671, 649401, 652917

-

3.1.1.6

cephalosporin C + H2O = deacetylcephalosporin C + acetate

0

-

3.1.1.72

cephalosporin C + H2O = deacetylcephalosporin C + acetate

649151, 0

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Enzyme Kinetic Parameters

K_M Value (2 results)

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Show KM Value (2 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.3.1.175

0.04

-

-

659547

2.3.1.175

0.29

-

-

658291

2.3.1.175

2 entries

References & Links

Literature References (16)

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Show Reference (16 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

170669

-

Deacetylcephalosporin C formation by cephalosporin C acetylhydrolase induced in a Cephalosporium acremonium

1975

Fujisawa, Y.; Shirafuji, H.; Kanzaki, T.

Agric. Biol. Chem.

39

1303-1309

170671

4519146

New findings on cephalosporin C biosynthesis

1973

Fujisawa, Y.; Shirafuji, H.; Kida, M.; Nara, K.

Nature New Biol.

246

154-155

170672

-

Purification and properties of cephalosporin-C deacetylase from the yeast, Rhodotorula glutinis 38B1, useful for bioconversion of 7-aminocephalosporanic acid derivatives

1998

Sakai, Y.; Abe, T.; Aoki, M.; Ohabayashi, Y.; Yamamoto, K.; Tani, Y.; Kato, N.

J. Ferment. Bioeng.

85

53-57

440380

2656705

Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus

1989

Dotzlaf, J.E.; Yeh, W.K.

J. Biol. Chem.

264

10219-10227

649151

11717513

Purification, crystallization and preliminary X-ray analysis of an acetylxylan esterase from Bacillus pumilus

2001

Benini, S.; Degrassi, G.; Krastanova, I.; Lamba, D.; Venturi, V.

Acta Crystallogr. Sect. D

57

1906-1907

649397

11030579

A cephalosporin C acetylhydrolase is present in the cultures of Nocardia lactamdurans

2000

Cardoza, R.E.; Velasco, J.; Martin, J.F.; Liras, P.

Appl. Microbiol. Biotechnol.

54

406-412

649401

11759684

Cloning and characterization of the gene cahB encoding a cephalosporin C acetylhydrolase from Acremonium chrysogenum

2001

Velasco, J.; Gutierrez, S.; Casqueiro, J.; Fierro, F.; Campoy, S.; Martin, J.F.

Appl. Microbiol. Biotechnol.

57

350-356

651823

-

Isolation, analysis, and expression of lipase with cephalosporin-C deacetylation activity from Staphylococcus sp

2001

Lee, H.W.; Ko, J.Y.; Kim, W.J.; Byun, S.M.

J. Biochem. Mol. Biol.

34

274-277

652917

12842474

Multifunctional xylooligosaccharide/cephalosporin C deacetylase revealed by the hexameric structure of the Bacillus subtilis enzyme at 1.9A resolution

2003

Vincent, F.; Charnock, S.J.; Verschueren, K.H.; Turkenburg, J.P.; Scott, D.J.; Offen, W.A.; Roberts, S.; Pell, G.; Gilbert, H.J.; Davies, G.J.; Brannigan, J.A.

J. Mol. Biol.

330

593-606

657617

9421924

Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum

1997

Gutierrez, S.; Velasco, J.; Marcos, A.T.; Fernandez, F.J.; Fierro, F.; Barredo, J.L.; Diez, B.; Martin, J.F.

Appl. Microbiol. Biotechnol.

48

606-614

657712

1632779

Cloning and disruption of the cefG gene encoding acetyl coenzyme A: deacetylcephalosporin C O-acetyltransferase from Acremonium chrysogenum

1992

Matsuda, A.; Sugiura, H.; Matsuyama, K.; Matsumoto, H.; Ichikawa, S.; Komatsu, K.

Biochem. Biophys. Res. Commun.

186

40-46

657802

9895280

Molecular characterization of the Acremonium chrysogenum cefG gene product: the native deacetylcephalosporin C acetyltransferase is not processed into subunits

1999

Velasco, J.; Gutierrez, S.; Campoy, S.; Martin, J.F.

Biochem. J.

337

379-385

658291

1368946

Purification of acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase from Acremonium chrysogenum

1992

Matsuyama, K.; Matsumoto, H.; Matsuda, A.; Sugiura, H.; Komatsu, K.; Ichikawa, S.

Biosci. Biotechnol. Biochem.

56

1410-1412

659547

-

Investigation of acetyl-CoA:deacetylcephalosporin C O-acetyltransferase of Cephalosporium acremonium

1985

Scheidegger, A.; Gutzwiller, A.; Kuenzi, M.T.; Fiechter, A.; Nuesch, J.

J. Biotechnol.

3

109-117

673028

17379148

Deacetylcephalosporin C production in Penicillium chrysogenum by expression of the isopenicillin N epimerization, ring expansion, and acetylation genes

2007

Ullan, R.V.; Campoy, S.; Casqueiro, J.; Fernandez, F.J.; Martin, J.F.

Chem. Biol.

14

329-339

688409

18279889

The last step in cephalosporin C formation revealed: crystal structures of deacetylcephalosporin C acetyltransferase from Acremonium chrysogenum in complexes with reaction intermediates

2008

Lejon, S.; Ellis, J.; Valegard, K.

J. Mol. Biol.

377

935-944

Links to other databases for deacetylcephalosporin C

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ChEBI

PubChem

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