Ligand 2-Hydroxyisovalerate

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Basic Ligand Information

Molecular Structure
Picture of 2-Hydroxyisovalerate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C5H10O3
2-Hydroxyisovalerate
NGEWQZIDQIYUNV-UHFFFAOYSA-M
Synonyms:
(R,S)-2-hydroxyisovalerate, 2-hydroxy-3-methylbutanoate, 2-hydroxy-3-methylbutyric acid, 3-methyl-2-hydroxybutanoate, DL-2-hydroxyisovalerate, valic acid

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
DL-2-hydroxyisovalerate + O2 = 2-oxoisovalerate + H2O2
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (8 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
2-hydroxyisovalerate + NAD+ = 2-oxoisovalerate + NADH + H+
show the reaction diagram
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(R,S)-2-hydroxyisovalerate + acceptor = 2-oxo-isovalerate + reduced acceptor
show the reaction diagram
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2-hydroxyisovalerate + O2 = ?
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (7 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
3-methyl-2-oxobutanoate + NADH = 2-hydroxy-3-methylbutanoate + NAD+
show the reaction diagram
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2-oxoisovalerate + NADH + H+ = 2-hydroxyisovalerate + NAD+
show the reaction diagram
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alpha-ketoisovalerate + NADH = 2-hydroxyisovalerate + NAD+
show the reaction diagram
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Inhibitor in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
10 mM, 31.9% inhibition
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (1 result)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.03
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach

KM Value (5 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.6
-
pH 8.0, 30°C

IC50 Value (1 result)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
8
-
pH 8, 22°C

References & Links

Links to other databases for 2-Hydroxyisovalerate

ChEBI
PubChem
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PubChem