Ligand 2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
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Basic Ligand Information
Molecular Structure

C20H27N3O15
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
ZSNJSHLEDPXSEP-UVFFFCKLSA-N
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (1 result)
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2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc + H2O = 2,4-dinitrophenol + beta-D-Gal-(1->3)-alpha-D-GalNAc
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (5 results)
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0.76
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pH 6.5, 37°C, mutant D764A
14
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pH 6.5, 37°C, mutant E796Q
18
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pH 6.5, 37°C, mutant E796A
541
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pH 6.5, 37°C, DELTA1-39 deletion mutant
748
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pH 6.5, 37°C, DELTA1-39,DELTA1568-1767 deletion mutant
KM Value (5 results)
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0.0012
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pH 6.5, 37°C, mutant E796Q
0.0013
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pH 6.5, 37°C, mutant E796A
0.033
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pH 6.5, 37°C, mutant D764A
0.034
-
pH 6.5, 37°C, DELTA1-39 deletion mutant
0.048
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pH 6.5, 37°C, DELTA1-39,DELTA1568-1767 deletion mutant
References & Links
Literature References (1)
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Mechanistic investigation of the endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae R6
2009
Willis, L.M.; Zhang, R.; Reid, A.; Withers, S.G.; Wakarchuk, W.W.
Biochemistry
48
10334-10341
Links to other databases for 2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc