Ligand 4-nitrophenyl beta-lactoside

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Basic Ligand Information

Molecular Structure
Picture of 4-nitrophenyl beta-lactoside (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C18H25NO13
4-nitrophenyl beta-lactoside
IAYJZWFYUSNIPN-XZILNXCWSA-N

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (5 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
at pH 5.0 and 25°C
0.78
-
pH 5.7, 37°C, mutant D214N
0.042
-
pH 5.7, 37°C, mutant E212Q
0.0041
-
pH 5.7, 37°C, mutant E217Q
0.00066
-
pH 5.7, 37°C, wild-type enzyme
3.5
-

KM Value (5 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
at pH 5.0 and 25°C
0.089
-
pH 5.7, 37°C, mutant D214N
0.37
-
pH 5.7, 37°C, mutant E212Q
0.27
-
pH 5.7, 37°C, mutant E217Q
0.21
-
pH 5.7, 37°C, wild-type enzyme
0.22
-

References & Links

Links to other databases for 4-nitrophenyl beta-lactoside

ChEBI
PubChem
-
PubChem