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Ligand L-isoleucyl-tRNALeu

Basic Ligand Information

Molecular Structure

Show all BRENDA pathways known for L-isoleucyl-tRNALeu

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Roles as Enzyme Ligand

In Vivo Product in Enzyme-catalyzed Reactions (2 results)

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Show Natural Product (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

6.1.1.3

ATP + L-isoleucine + tRNAIle = AMP + diphosphate + L-isoleucyl-tRNAIle

652046

-

6.1.1.5

ATP + L-isoleucine + tRNAIle = AMP + diphosphate + L-isoleucyl-tRNAIle

650527, 651903, 651904, 651905, , 652205, 650559, 650560, 650564, 652415, 653886, 652566, 652046

-

Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Substrate (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

6.1.1.4

L-isoleucyl-tRNALeu + H2O = t-RNALeu + isoleucine

702063

-

6.1.1.4

L-isoleucyl-tRNALeu + H2O = t-RNALeu + L-isoleucine

702347

-

6.1.1.4

2 entries

Product in Enzyme-catalyzed Reactions (4 results)

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Show Product (4 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

6.1.1.3

ATP + L-isoleucine + tRNAIle = AMP + diphosphate + L-isoleucyl-tRNAIle

652046

-

6.1.1.4

ATP + L-isoleucine + tRNALeu = AMP + diphosphate + L-isoleucyl-tRNALeu

, 653192, 649992

-

6.1.1.5

ATP + L-isoleucine + tRNAIle = AMP + diphosphate + L-isoleucyl-tRNAIle

652046, 650527, 651903, 651904, 651905, , 67, 466, 652205, 447, 448, 449, 450, 452, 453, 454, 455, 456, 461, 462, 463, 464, 465, 467, 468, 471, 476, 650559, 650560, 650564, 470, 458, 469, 472, 459, 475, 474, 652415, 653886, 652566, 460, 451, 457

-

6.1.1.9

ATP + L-isoleucine + tRNAVal = AMP + diphosphate + L-isoleucyl-tRNAVal

650085

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (4 results)

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Show Turnover Number (4 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

6.1.1.4

0.19

-

mutant Y515A, pH 7.5, 37°C

702063

6.1.1.4

0.48

-

mutant Y520A, pH 7.5, 37°C

702063

6.1.1.4

0.93

-

mutant Y520H, pH 7.5, 37°C

702063

6.1.1.4

1.52

-

wild-type, pH 7.5, 37°C

702063

6.1.1.4

4 entries

K_M Value (4 results)

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Show KM Value (4 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

6.1.1.4

0.0092

-

wild-type, pH 7.5, 37°C

702063

6.1.1.4

0.0122

-

mutant Y515A, pH 7.5, 37°C

702063

6.1.1.4

0.0147

-

mutant Y520H, pH 7.5, 37°C

702063

6.1.1.4

0.0173

-

mutant Y520A, pH 7.5, 37°C

702063

6.1.1.4

4 entries

References & Links

Literature References (47)

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Show Reference (47 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

67

6468656

Thermostable valyl-tRNA, isoleucyl-tRNA and methionyl-tRNA synthetases from an extreme thermophile Thermus thermophilus HB8. Protein structure and Zn2+ binding

1984

Kohda, D.; Yokoyama, S; Miyazawa, T.

FEBS Lett.

174

20-23

447

7929087

Relationship of protein structure of isoleucyl-tRNA synthetase with pseudomonic acid resistance of Escherichia coli

1994

Yanagisawa, T.; Lee, J.T.; Wu, H.C.; Kawakami, M.

J. Biol. Chem.

269

24304-24309

448

8672449

C-terminal zinc-containing peptide required for RNA recognition by a class I tRNA synthetase

1996

Glasfeld, E.; Landro, J.A.; Schimmel, P.

Biochemistry

35

4139-4145

449

8692688

Proofreading in trans by an aminoacyl-tRNA synthetase. A model for single site editing by isoleucyl-tRNA synthetase

1996

Jakubowski, H.

Nucleic Acids Res.

24

2505-2510

450

7488160

Mutation of the carboxy terminal zinc finger of E. coli isoleucyl-tRNA synthetase alters zinc binding and aminoacylation activity

1995

Zhou, L.; Rosevear, P.R.

Biochem. Biophys. Res. Commun.

216

648-654

451

7701322

Switching recognition of two tRNA synthetases with an amino acid swap in a designed peptide

1995

Auld, D.S.; Schimmel., P.

Science

267

1994-1996

452

7947832

Thiol ligation of two zinc atoms to a class I tRNA synthetase. Evidence for unshared thiols and role in amino acid binding and utilization

1994

Landro, J.A.; Schmidt, E.; Schimmel, P.

Biochemistry

33

14213-14220

453

8286369

Probing the metal binding sites of E. coli isoleucyl-tRNA synthetase

1994

Xu, B.; Trawick, B.; Krudy, G.A.; Phillips, R.M.; Zhou.L.; Rosevear, P.R.

Biochemistry

33

398-402

454

1459130

Analysis of the isoleucyl-tRNA synthetase reaction by total rate equation. Magnesium and spermidine in the tRNA kinetics

1992

Airas, R.K.

Eur. J. Biochem.

210

451-454

455

8129874

Evidence for distinct locations for metal binding sites in two closely related class I tRNA synthetases

1993

Schimmel, P.; Landro, J.A.; Schidt, E.

J. Biomol. Struct. Dyn.

11

571-581

456

9184155

Zinc-dependent tRNA binding by a peptide element within a tRNA synthetase

1997

Glasfeld, E.; Schimmel, P.

Biochemistry

36

6739-6744

457

1910039

Expression and characterization of a recombinant yeast isoleucyl-tRNA synthetase

1991

Racher, K.I.; Kalmar, G.B.; Borgford, T.J.

J. Biol. Chem.

266

17158-17164

458

3311074

Structure of the yeast isoleucyl-tRNA synthetase gene (ILS1). DNA-sequence, amino-acid sequence of the proteolytic peptides of the enzyme and comparison of the structure to those of other known aminoacyl-tRNA synthetases

1987

Englisch, U.; Englisch, S.; Markmeyer, P.; Schischkoff, J.; Sterbach, H.; Kratzin, H.; Cramer, F.

Biol. Chem. Hoppe-Seyler

368

971-979

459

2037598

Isoleucyl-tRNA synthetase of Methanobacterium thermoautotrophicum Marburg. Cloning of the gene, nucleotide sequence, and localization of a base change conferring resistance to pseudomonic acid

1991

Jenal.U.; Rechsteiner, T.; Tan, P.Y.; Buhlmann, E.; Meile, L.; Leisinger, T.

J. Biol. Chem.

266

10570-10577

460

4336851

Isoleucyl-transfer ribonucleic acid synthetase from Bacillus stearothermophilus. Properties of the enzyme

1972

Charlier, J.; Grosjean, H.

Eur. J. Biochem.

25

163-174

461

11946158

Comparative studies on the isoleucyl-tRNA synthetases from Bacillus stearothermophilus and Escherichia coli. II. The effect of magnesium ions in the transacylation reaction

1971

Grosjean, H.; Charlier, J.

FEBS Lett.

18

342-346

462

4334974

Cation requirements of isoleucyl-tRNA synthetase from Escherichia coli

1972

Steinmetz Kayne , M.; Cohn, M.

Biochem. Biophys. Res. Commun.

46

1285-1291

463

4569771

Isoleucyl transfer ribonucleic acid synthetase of Escherichia coli B. Effects of magnesium and spermine on the amino acid activation reaction

1973

Holler, E.

Biochemistry

12

1142-1149

464

4581276

Isolation and properties of isoleucyl-tRNA synthetase from Escherichia coli MRE 600

1973

Durekovic, A.; Flossdorf, J.; Kula, M.R.

Eur. J. Biochem.

36

528-533

465

238842

The effect of spermine anf Mg2+ on the catalytic mechanism of isoleucine:tRNA ligase

1975

Carr, A.C.; Igloi, G.L.; Penzer, G.R.; Plumbridge, J.A.

Eur. J. Biochem.

54

169-173

466

-

Studies on isoleucyl-tRNA synthetase of an extreme thermophile, Thermus thermophilus HB8

1975

Wakagi, T.; Ohta, T.; Imahori, K.

Agric. Biol. Chem.

39

1573-1580

467

-

L-Isoleucyl-tRNA synthetase aus E. coli. pH-Abhängigkeit der Enzym-Substrat Komplexbildung

1976

Hammer-Raber, B.; Rainey, P.M.; Holler, E.

Hoppe-Seyler's Z. Physiol. Chem.

357

295

468

793865

Purification of isoleucyl transfer ribonucleic acid synthetase by affinity chromatography on blue dextran-Sepharose

1976

Moe, J.G.; Piszkiewicz, D.

FEBS Lett.

72(1)

147-150

469

782885

Valyl-tRNA, isoleucyl-tRNA and tyrosyl-tRNA synthetase from baker's yeast. Substrate specificity with regard to ATP analogs and mechanism of the aminoacylation reaction

1976

Freist, W.; von der Haar, F.; Faulhammer, H.; Cramer, F.

Eur. J. Biochem.

66

493-497

470

244419

Effect of polyamines on isoleucyl-tRNA formation by rat liver isoleucyl-tRNA synthetase

1978

Igarashi, K.; Eguchi, K.; Tanaka, M.; Hirose, S.

Eur. J. Biochem.

82

301-307

471

365175

Inhibition of isoleucyl-transfer ribonucleic acid synthetase in Escherichia coli by pseudomonic acid

1978

Hughes, J.; Mellows, G.

Biochem. J.

176

305-318

472

6281001

Isoleucyl-tRNA synthetase from baker's yeast

1981

Freist, W.; von der Haar, F.; Cramer, F.

Eur. J. Biochem.

119

151-164

474

4074679

Purification and characterization of the isoleucyl-tRNA synthetase component from the high molecular weight complex of sheep liver. A hydrophobic metalloprotein

1985

Lazard, M.; Mirande, M.; Waller, J.P.

Biochemistry

24

5099-5106

475

-

Mutation to pseudomonic acid resistance of Methanobacterium thermoautotrophicum leads to an altered isoleucyl-tRNA synthetase

1986

Kiener, A.; Rechsteiner, T.; Leisinger, T.

FEMS Microbiol. Lett.

33

15-18

476

3315663

Isoleucyl-tRNA synthetase from baker's yeast and from Escherichia coli MRE 600. Discrimination of 20 amino acids in aminoacylation of tRNAIle-C-C-A(3'NH2)

1987

Freist, W.; Sternbach, H.; Cramer, F.

Eur. J. Biochem.

169

33-39

649992

10828991

CP1 domain in Escherichia coli leucyl-tRNA synthetase is crucial for its editing function

2000

Chen, J.F.; Guo, N.N.; Li, T.; Wang, E.D.; Wang, Y.L.

Biochemistry

39

6726-6731

650085

11434781

Misacylation and editing by Escherichia coli valyl-tRNA synthetase: evidence for two tRNA binding sites

2001

Tardif, K.D.; Liu, M.; Vitseva, O.; Hou, Y.M.; Horowitz, J.

Biochemistry

40

8118-8125

650527

12788342

Synthesis and activity of analogues of the isoleucyl tRNA synthetase inhibitor SB-203207

2003

Crasto, C.F.; Forrest, A.K.; Karoli, T.; March, D.R.; Mensah, L.; O'Hanlon, P.J.; Nairn, M.R.; Oldham, M.D.; Yue, W.; Banwell, M.G.; Easton, C.J.

Bioorg. Med. Chem.

11

2687-2694

650559

11327600

Ester and hydroxamate analogues of methionyl and isoleucyl adenylates as inhibitors of methionyl-tRNA and isoleucyl-tRNA synthetases

2001

Lee, J.; Kang, S.U.; Kim, S.Y.; Kim, S.E.; Kang, M.K.; Jo, Y.J.; Kim, S.

Bioorg. Med. Chem. Lett.

11

961-964

650560

11327601

Vanilloid and isovanilloid analogues as inhibitors of methionyl-tRNA and isoleucyl-tRNA synthetases

2001

Lee, J.; Kang, S.U.; Kim, S.Y.; Kim, S.E.; Job, Y.J.; Kim, S.

Bioorg. Med. Chem. Lett.

11

965-968

650564

12643918

N-Alkoxysulfamide, N-hydroxysulfamide, and sulfamate analogues of methionyl and isoleucyl adenylates as inhibitors of methionyl-tRNA and isoleucyl-tRNA synthetases

2003

Lee, J.; Kim, S.E.; Lee, J.Y.; Kim, S.Y.; Kang, S.U.; Seo, S.H.; Chun, M.W.; Kang, T.; Choi, S.Y.; Kim, H.O.

Bioorg. Med. Chem. Lett.

13

1087-1092

651903

9822629

Characterization of isoleucyl-tRNA synthetase from Staphylococcus aureus. I: Kinetic mechanism of the substrate activation reaction studied by transient and steady-state techniques

1998

Pope, A.J.; Lapointe, J.; Mensah, L.; Benson, N.; Brown, M.J.; Moore, K.J.

J. Biol. Chem.

273

31680-31690

651904

9822630

Characterization of isoleucyl-tRNA synthetase from Staphylococcus aureus. II. Mechanism of inhibition by reaction intermediate and pseudomonic acid analogues studied using transient and steady-state kinetics

1998

Pope, A.J.; Moore, K.J.; McVey, M.; Mensah, L.; Benson, N.; Osbourne, N.; Broom, N.; Brown, M.J.; O'Hanlon, P.

J. Biol. Chem.

273

31691-31701

651905

9822631

Effects of substrate and inhibitor binding on proteolysis of isoleucyl-tRNA synthetase from Staphylococcus aureus

1998

Pope, A.J.; McVey, M.; Fantom, K.; Moore, K.J.

J. Biol. Chem.

273

31702-31706

652046

10930398

Active aminoacyl-tRNA synthetases are present in nuclei as a high molecular weight multienzyme complex

2000

Nathanson, L.; Deutscher, M.P.

J. Biol. Chem.

275

31559-31562

652205

11584022

Structural basis for the recognition of isoleucyl-adenylate and an antibiotic, mupirocin, by isoleucyl-tRNA synthetase

2001

Nakama, T.; Nureki, O.; Yokoyama, S.

J. Biol. Chem.

276

47387-47393

652415

12672810

How does Pseudomonas fluorescens avoid suicide from its antibiotic pseudomonic acid?: Evidence for two evolutionarily distinct isoleucyl-tRNA synthetases conferring self-defense

2003

Yanagisawa, T.; Kawakami, M.

J. Biol. Chem.

278

25887-25894

652566

14672940

Crystal structures of the CP1 domain from Thermus thermophilus isoleucyl-tRNA synthetase and its complex with L-valine

2004

Fukunaga, R.; Fukai, S.; Ishitani, R.; Nureki, O.; Yokoyama, S.

J. Biol. Chem.

279

8396-8402

653192

12718881

Structural and mechanistic basis of pre- and posttransfer editing by leucyl-tRNA synthetase

2003

Lincecum, T.L., Jr.; Tukalo, M.; Yaremchuk, A.; Mursinna, R.S.; Williams, A.M.; Sproat, B.S.; Van Den Eynde, W.; Link, A.; Van Calenbergh, S.; Grotli, M.; Martinis, S.A.; Cusack, S.

Mol. Cell

11

951-963

653886

10446055

Insights into editing from an Ile-tRNA synthetase structure with tRNAIle and mupirocin

1999

Silvian, L.F.; Wang, J.; Steitz, T.A.

Science

285

1074-1077

702063

19540202

Two tyrosine residues outside the editing active site in Giardia lamblia leucyl-tRNA synthetase are essential for the post-transfer editing

2009

Zhou, X.L.; Wang, E.D.

Biochem. Biophys. Res. Commun.

386

510-515

702347

19702327

A paradigm shift for the amino acid editing mechanism of human cytoplasmic leucyl-tRNA synthetase

2009

Pang, Y.L.; Martinis, S.A.

Biochemistry

48

8958-8964

Links to other databases for L-isoleucyl-tRNALeu

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Show Links (1 entry)