Ligand hydrazine

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Basic Ligand Information

Molecular Structure
Picture of hydrazine (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
H4N2
hydrazine
OAKJQQAXSVQMHS-UHFFFAOYSA-N
Synonyms:
H2NNH2, hydrazin, N2H4, NH2NH2

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
hydrazine + reduced ferredoxin = 2 NH3 + oxidized ferredoxin
show the reaction diagram
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hydrazine + H2O + ferricytochrome c = nitric oxide + ammonium + ferrocytochrome c
show the reaction diagram
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hydrazine + ferricytochrome c = N2 + ferrocytochrome c
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
N2 + 4 reduced ferredoxin = hydrazine + 4 oxidized ferredoxin
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (27 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
hydrazine + H2O + ferricytochrome c = nitric oxide + ammonium + ferrocytochrome c
show the reaction diagram
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short-chain amides + hydrazine = acid hydrazides + NH3
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hydrazine + fumarate = 2-hydrazinosuccinic acid
show the reaction diagram
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mesaconate + hydrazine = ?
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ATP + hydrazine + HCO3- = ATP + phosphate + N-amino carbamoyl phosphate
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (16 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
N2 + 4 reduced ferredoxin = hydrazine + 4 oxidized ferredoxin
show the reaction diagram
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L-glutamylhydrazine + methylamine = gamma-L-glutamylmethylamide + hydrazine
show the reaction diagram
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(gamma-L-glutamyl)hydrazine + H2O = L-glutamate + hydrazine
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L-leucinhydrazide + H2O = L-leucine + H2NNH2
show the reaction diagram
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phenylacetic hydrazide + H2O = phenylacetate + hydrazine
show the reaction diagram
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Inhibitor in Enzyme-catalyzed Reactions (56 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
inhibitory at 100 mM
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1 mM, 41% inhibition
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77% residual activity at 1 mM
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5 mM, 45% residual activity
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uncompetitive; uncompetitive inhibition, inhibition constant: 0.3 mM
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and derivatives
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1-3 mM
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1-3 mM
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protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism
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1 mM, 28% residual activity
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complete inhibition at 1 mM
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10 mM, 95% inhibition
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1 mM, complete inhibition
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competes strongly with hydroxylamine. Addition of 0.015 mM, 0.035 mM, and 0.35 mM results in 15%, 50%, and 85% inhibition, respectively
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inactivates wild-type hcAMPP and R535A mutant enzymes
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1 mM
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25 mM, complete inhibition
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1 mM, 82% inhibition
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3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (3 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
3
6
at pH 7.0 and 37°C

KM Value (8 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

Ki Value (4 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.35
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References & Links

Links to other databases for hydrazine

ChEBI
PubChem
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PubChem