Any feedback?
BRENDA home
Ligand Avicel
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information
close all tables 
open all tablesRoles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (37 results)
top
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EC NUMBER 
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
avicel + ascorbate + O2 = C1-oxidized cellooligosaccharides + dehydroascorbate + H2O
-
avicel + ascorbate + O2 = C4-oxidized cellooligosaccharides + C1/C4-oxidized cellooligosaccharides + dehydroascorbate + H2O
-
avicel + H2O = ?
731793, 731310, 731498, 743930, 745353, 747350, 750135, 751125, 742545, 695830, 664783, 744426, 735810, 750082, 732818, 735513, 744104, 742763, 744550, 750136, 744885
-
avicel + H2O = cellobiose + ?
-
avicel + H2O = ?
736781, 656333, 326312, 393618, 682688, 745048, 393593, 742404, 742545, 393560, 393640, 750440, 677720, 693294, 678809, 723993, 657034, 730175, 743720, 743723, 735807, 736553, 744810, 744076, 393619, 393631, 393636, 735579, 655841, 655859, 655827, 680314, 393597, 393559, 393620, 393627, 393579, 678759, 677608, 680983, 681360, 693995, 694990, 736374, 736149, 737199, 740175, 741842, 750783
-
Avicel + H2O = D-glucose + cellotetraose + cellotriose + cellobiose
-
avicel + H2O = ?
713792, 731342, 750524, 665332, 678845, 752111, 752089, 663567, 663568, 742765, 750082, 696830, 709052, 707767, 715879, 747349
-
avicel + H2O = cellobiose
-
Activator in Enzyme-catalyzed Reactions (1 result)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
Enzyme Kinetic Parameters
kcat Value (Turnover Number) (4 results)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
KM Value (9 results)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
-999
-
Km value of the full-length enzyme increases from 2.8 g/l, pH 5.0, 10°C, to 19 g/l, pH 5.0, 50°C
-999
-
Km value of the full-length enzyme increases from 2.8 g/l, pH 5.0, 10°C, to 29 g/l, pH 5.0, 50°C
-999
-
Km value of the isolated catalytic domain increases from 4.9 g/l, pH 5.0, 10°C, to 76 g/l, pH 5.0, 50°C
-999
-
Km value of the isolated catalytic domain increases from 7.1 g/l, pH 5.0, 10°C, to 122 g/l, pH 5.0, 50°C
References & Links
Literature References (159)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
BRENDA REFERENCE ID
PUBMED ID
TITLE
YEAR
AUTHORS
JOURNAL
VOLUME
PAGES
-
Glucohydrolase from Penicillium funiculosum
1989
Rao, M.; Mishra, C.
Appl. Microbiol. Biotechnol.
30
130-134
Extracellular enzyme system utilized by the fungus Sporotrichum pulverulentum (Chrysosporium lignorum) for the breakdown of cellulose. 3. Purification and physico-chemical characterization of an exo-1,4-beta-glucanase
1975
Eriksson, K.E.; Petterson, B.
Eur. J. Biochem.
51
213-218
Purification, characterization and gene analysis of exo-cellulase II (Ex-2) from the white rot basidiomycete Irpex lacteus
1999
Hamada, N.; Ishikawa, K.; Fuse, N.; Kodaira, R.; Shimosaka, M.; Amano, Y.; Kanda, T.; Okazaki, M.
J. Biosci. Bioeng.
87
442-451
Properties and gene structure of a bifunctional celluloytic enzyme (CelA) from the extreme thermophile 'Anaerocellum thermophilum' with separate glycosyl hydrolase family 9 and 48 catalytic domains
1998
Zverlov, V.; Mahr, S.; Riedel, K.; Bronnenmeier, K.
Microbiology
144
457-465
-
Cellobiosidase from Ruminococcus albus
1988
Ohmiya, K.; Shimizu, S.
Methods Enzymol.
160
391-398
-
Cellulases of a mutant strain of Trichoderma viride QM 9414
1988
Voragen, A.G.J.; Beldman, G.; Rombouts, F.M.
Methods Enzymol.
160
243-251
-
Purification and properties of an extracellular beta-glucosidase from the cellulolytic thermophile Clostridium stercorarium
1988
Bronnenmeier, K.; Staudenbauer, W.L.
Appl. Microbiol. Biotechnol.
28
380-386
The cellulase of Trichoderma viride. Purification, characterization and comparison of all detectable endoglucanases, exoglucanases and beta-glucosidases
1985
Beldman, G.; Searle-Van Leeuwen, M.F.; Rombouts, F.M.; Voragen, F.G.J.
Eur. J. Biochem.
146
301-308
-
Cloning and expression of a Clostridium cellobioparum cellulase gene and its excretion from Escherichia coli JM 109
1989
Shima, S.; Kato, J.; Igarashi, Y.; Kodama, T.
J. Ferment. Bioeng.
68
75-78
Purification and properties of an endo-1,4-beta-glucanase from Clostridium josui
1989
Fujino, T.; Sukhumavasi, J.; Sasaki, T.; Ohmiya, K.; Shimizu, S.
J. Bacteriol.
171
4076-4079
-
Endoglucanase from Clostridium thermocellum
1988
Ng, T.K.; Zeikus, J.G.
Methods Enzymol.
160
351-355
Characterization of the purified multifunctional cellulase component of Penicillium funiculosum
1987
Sahasrabudhe, N.A.; Lachke, A.H.; Ranjekar, P.K.
Biotechnol. Lett.
9
881-886
-
Isolation, crystallization and properties of a new cellulase of Clostridium thermocellum overproduced in Escherichia coli
1986
Joliff, G.; Beguin, P.; Juy, M.; Millet, J.; Ryter, A.; Poljak, R.; Aubert, J.P.
Bio/Technology
4
896-900
Enzymatic studies on a cellulase system of Trichoderma viride. IV. Purification and properties of a less-random type cellulase
1976
Okada, G.
J. Biochem.
80
913-922
Purification and properties of an endo-cellulase of avicelase type from Irpex lacteus (Polyporus tulipiferae)
1976
Kanda, T.; Wakabayashi, K.; Nisizawa, K.
J. Biochem.
79
977-988
Studies on cellulase produced by the phytopathogens. Purification and enzymatic properties of cellulase of Fusarium moniliforme
1974
Matsumoto, K.; Endo, Y.; Tamiya, N.; Kano, M.; Miyauchi, K.; Abe, J.
J. Biochem.
76
563-572
An internal cellulose-binding domain mediates adsorption of an engineered bifunctional xylanase/cellulase
1994
Tomme, P.; Gilkes, N.R.; Miller Jr., R.C.; Warren, A.J.; Kilburn, D.G.
Protein Eng.
7
117-123
Molecular cloning and expression of an endo-beta-1,4-D-glucanase I (avicelase I) gene from Bacillus cellulolyticus K-12 and characterization of the recombinant enzyme
1999
Lee, T.K.; Kim, C.H.
Appl. Biochem. Biotechnol.
80
121-125
Characterization and substrate specificity of an endo-beta-1,4-D-glucanase I (avicelase I) from an extracellular multienzyme complex of Bacillus circulans
1995
Kim, C.H.
Appl. Environ. Microbiol.
61
959-965
Cloning and expression of a beta-1,4-endoglucanase gene from Cellulomonas sp. CelB7 in Escherichia coli. Purification and characterization of the recombinant enzyme
1996
Fueloep, L.; Tran, S.L.P.; Pragai, Z.; Felfoeldi, F.; Ponyi, T.
FEMS Microbiol. Lett.
145
355-360
-
Cellulases of Cellulomonas uda
1988
Nakamura, K.; Kitamura, K.
Methods Enzymol.
160
211-216
-
Exocellulase of Irpex lacteus (Polyporus tulipiferae)
1988
Kanda, T.; Nisizawa, K.
Methods Enzymol.
160
403-408
-
Purification and some properties of two cellobiohydrolases from Penicillium purpurogenum
1986
Kamagata, Y.; Sasaki, H.; Takao, S.
J. Ferment. Technol.
64
211-217
-
Purification and some properties of cellulose 1,4-beta-cellobiosidase from a strain of Penicillium sp.
1986
Funaguma, T.; Tsuji, H.; Hara, A.
J. Ferment. Technol.
64
77-80
-
Purification and properties of two exocellobiohydrolases from a cellulolytic culture of Fusarium lini
1983
Mishra, C.; Vaidya, M.; Rao, M.; Deshpande, V.
Enzyme Microb. Technol.
5
430-434
Product inhibition of the recombinant CelS, and exoglucanase component of the Clostridium thermocellum cellulosome
1995
Kruus, K.; Andreacchi, A.; Wang, W.K.; Wu, J.H.D.
Appl. Microbiol. Biotechnol.
44
399-404
Isolation of a cellobiohydrolase of Clostridium thermocellum capable of degrading natural crystalline substrates
1993
Singh, R.N.; Akimenko, V.K.
Biochem. Biophys. Res. Commun.
192
1123-1130
Purification and properties of a novel type of exo-1,4-beta-glucanase (avicelase II) from the cellulolytic thermophile Clostridium stercorarium
1991
Bronnenmeier, K.; Rucknagel, K.P.; Staudenbauer, W.L.
Eur. J. Biochem.
200
379-385
Structural Basis for the exocellulase activity of the cellobiohydrolase CbhA from Clostridium thermocellum
2004
Schubot, F.D.; Kataeva, I.A.; Chang, J.; Shah, A.K.; Ljungdahl, L.G.; Rose, J.P.; Wang, B.C.
Biochemistry
43
1163-1170
CelE, a multidomain cellulase from Clostridium cellulolyticum: a key enzyme in the cellulosome?
2000
Gaudin, C.; Belaich, A.; Champ, S.; Belaich, J.P.
J. Bacteriol.
182
1910-1915
Cel9M, a new family 9 cellulase of the Clostridium cellulolyticum cellulosome
2002
Belaich, A.; Parsiegla, G.; Gal, L.; Villard, C.; Haser, R.; Belaich, J.P.
J. Bacteriol.
184
1378-1384
Characterization of a cellulase containing a family 30 carbohydrate-binding module (CBM) derived from Clostridium thermocellum CelJ: importance of the CBM to cellulose hydrolysis
2003
Arai, T.; Araki, R.; Tanaka, A.; Karita, S.; Kimura, T.; Sakka, K.; Ohmiya, K.
J. Bacteriol.
185
504-512
Role of cellulose-binding domain of exocellulase I from white rot basidiomycete Irpex lacteus
2001
Hamada, N.; Kodaira, R.; Nogawa, M.; Shinji, K.; Ito, R.; Amano, Y.; Shimosaka, M.; Kanda, T.; Okazaki, M.
J. Biosci. Bioeng.
91
359-362
Purification and characterization of cellulases produced by two Bacillus strains
2000
Mawadza, C.; Hatti-Kaul, R.; Zvauya, R.; Mattiasson, B.
J. Biotechnol.
83
177-187
Characterization of a functional soluble form of a Brassica napus membrane-anchored endo-1,4-beta-glucanase heterologously expressed in Pichia pastoris
2001
Molhoj, M.; Ulvskov, P.; Dal Degan, F.
Plant Physiol.
127
674-684
-
Cloning and expression of Phanerochaete chrysosporium cellobiohydrolase (cbhl.2)
2005
Howard, R.L.
Afr. J. Biotechnol.
4
1180-1184
-
Refolding and characterization of a heterologous expressed Phanerochaete chrysosporium cellobiohydrolase (CBHl.2)
2005
Howard, R.L.
Afr. J. Biotechnol.
4
1185-1188
-
Purification, cloning and characterisation of two forms of thermostable and highly active cellobiohydrolase I (Cel7A) produced by the industrial strain of Chrysosporium lucknowense
2005
Gusakov, A.V.; Sinitsyn, A.P.; Salanovich, T.N.; Bukhtojarov, F.E.; Markov, A.V.; Ustinov, B.B.; van Zeijl, C.; Punt, P.; Burlingame, R.
Enzyme Microb. Technol.
36
57-69
-
Expression of a cellobiohydrolase cDNA from the white rot fungus Irpex lacteus in Aspergillus oryzae
2005
Toda, H.; Takada, S.; Amano, Y.; Kanda, T.; Okazaki, M.; Shimosaka, M.
J. Appl. Glycosci.
52
23-26
Comparison of wild-type and UV-mutant beta-glucanase-producing strains of Talaromyces emersonii with potential in brewing applications
2005
McCarthy, T.C.; Lalor, E.; Hanniffy, O.; Savage, A.V.; Tuohy, M.G.
J. Ind. Microbiol. Biotechnol.
32
125-134
Salt-activated endoglucanase of a strain of alkaliphilic Bacillus agaradhaerens
2006
Hirasawa, K.; Uchimura, K.; Kashiwa, M.; Grant, W.D.; Ito, S.; Kobayashi, T.; Horikoshi, K.
Antonie van Leeuwenhoek
89
211-219
Properties of cellulosomal family 9 cellulases from Clostridium cellulovorans
2006
Arai, T.; Kosugi, A.; Chan, H.; Koukiekolo, R.; Yukawa, H.; Inui, M.; Doi, R.H.
Appl. Microbiol. Biotechnol.
71
654-660
Purification and characterization of cellulase produced by Bacillus amyoliquefaciens DL-3 utilizing rice hull
2008
Lee, Y.J.; Kim, B.K.; Lee, B.H.; Jo, K.I.; Lee, N.K.; Chung, C.H.; Lee, Y.C.; Lee, J.W.
Biores. Technol.
99
378-386
Analysis of the putative substrate binding region of hyperthermophilic endoglucanase from Pyrococcus horikoshii
2007
Kim, H.W.; Takagi, Y.; Hagihara, Y.; Ishikawa, K.
Biosci. Biotechnol. Biochem.
71
2585-2587
A functionally based model for hydrolysis of cellulose by fungal cellulase
2006
Zhang, Y.H.; Lynd, L.R.
Biotechnol. Bioeng.
94
888-898
Design of highly efficient cellulase mixtures for enzymatic hydrolysis of cellulose
2007
Gusakov, A.V.; Salanovich, T.N.; Antonov, A.I.; Ustinov, B.B.; Okunev, O.N.; Burlingame, R.; Emalfarb, M.; Baez, M.; Sinitsyn, A.P.
Biotechnol. Bioeng.
97
1028-1038
Expression and purification of cellulase Xf818 from Xylella fastidiosa in Escherichia coli
2006
Wulff, N.A.; Carrer, H.; Pascholati, S.F.
Curr. Microbiol.
53
198-203
-
Heterologous expression of Melanocarpus albomyces cellobiohydrolase Cel7B, and random mutagenesis to improve its thermostability
2007
Voutilainen, S.P.; Boer, H.; Linder, M.B.; Puranen, T.; Rouvinen, J.; Vehmaanperae, J.; Koivula, A.
Enzyme Microb. Technol.
41
234-243
A xylan-degrading strain of Sulfolobus solfataricus: isolation and characterization of the xylanase activity
2004
Cannio, R.; Di Prizito, N.; Rossi, M.; Morana, A.
Extremophiles
8
117-124
Activation of crystalline cellulose to cellulose III(I) results in efficient hydrolysis by cellobiohydrolase
2007
Igarashi, K.; Wada, M.; Samejima, M.
FEBS J.
274
1785-1792
Correlation between cellulose binding and activity of cellulose-binding domain mutants of Humicola grisea cellobiohydrolase 1
2007
Takashima, S.; Ohno, M.; Hidaka, M.; Nakamura, A.; Masaki, H.; Uozumi, T.
FEBS Lett.
581
5891-5896
Purification and characterization of a new endoglucanase from Aspergillus aculeatus
2007
Naika, G.S.; Kaul, P.; Prakash, V.
J. Agric. Food Chem.
55
7566-7572
Comparative characterization of a recombinant Volvariella volvacea endoglucanase I (EG1) with its truncated catalytic core (EG1-CM), and their impact on the bio-treatment of cellulose-based fabrics
2007
Wu, S.; Ding, S.; Zhou, R.; Li, Z.
J. Biotechnol.
130
364-369
Effect of the cellulose-binding domain on the catalytic activity of a beta-glucosidase from Saccharomycopsis fibuligera
2007
Gundllapalli, S.B.; Pretorius, I.S.; Cordero Otero, R.R.
J. Ind. Microbiol. Biotechnol.
34
413-421
The brown-rot basidiomycete Fomitopsis palustris has the endo-glucanases capable of degrading microcrystalline cellulose
2007
Yoon, J.J.; Cha, C.J.; Kim, Y.S.; Son, D.W.; Kim, Y.K.
J. Microbiol. Biotechnol.
17
800-805
Purification and characterization of recombinant endoglucanase of Trichoderma reesei expressed in Saccharomyces cerevisiae with higher glycosylation and stability
2008
Qin, Y.; Wei, X.; Liu, X.; Wang, T.; Qu, Y.
Protein Expr. Purif.
58
162-167
Hydrolysis of amorphous and crystalline cellulose by heterologously produced cellulases of Melanocarpus albomyces
2008
Szijarto, N.; Siika-Aho, M.; Tenkanen, M.; Alapuranen, M.; Vehmaanperae, J.; Reczey, K.; Viikari, L.
J. Biotechnol.
136
140-147
Cellulase kinetics as a function of cellulose pretreatment
2008
Bommarius, A.S.; Katona, A.; Cheben, S.E.; Patel, A.S.; Ragauskas, A.J.; Knudson, K.; Pu, Y.
Metab. Eng.
10
370-381
Production, purification and characterization of beta-1,4-endoglucanase from a novel bacterial strain CTP-09 of a Bacillus sp
2008
Saleem, M.; Akhtar, M.S.; Yasmin, R.; Zahid, M.; Malik, N.N.; Afzal, M.; Rajoka, M.I.
Protein Pept. Lett.
15
402-410
Heterologous expression, crystallization and preliminary X-ray characterization of CcCel6C, a glycoside hydrolase family 6 enzyme from the basidiomycete Coprinopsis cinerea
2009
Kurakata, Y.; Tonozuka, T.; Liu, Y.; Kaneko, S.; Nishikawa, A.; Fukuda, K.; Yoshida, M.
Acta Crystallogr. Sect. F
65
140-143
Cloning and functional characterization of a complex endo-beta-1,3-glucanase from Paenibacillus sp.
2009
Cheng, Y.M.; Hong, T.Y.; Liu, C.C.; Meng, M.
Appl. Microbiol. Biotechnol.
81
1051-1061
Improving the thermostability and activity of Melanocarpus albomyces cellobiohydrolase Cel7B
2009
Voutilainen, S.P.; Boer, H.; Alapuranen, M.; Jaenis, J.; Vehmaanperae, J.; Koivula, A.
Appl. Microbiol. Biotechnol.
83
261-272
Gene cloning of cellobiohydrolase II from the white rot fungus Irpex lacteus MC-2 and its expression in Pichia pastoris
2008
Toda, H.; Nagahata, N.; Amano, Y.; Nozaki, K.; Kanda, T.; Okazaki, M.; Shimosaka, M.
Biosci. Biotechnol. Biochem.
72
3142-3147
Cloning, expression, and characterization of novel thermostable family 7 cellobiohydrolases
2008
Voutilainen, S.P.; Puranen, T.; Siika-Aho, M.; Lappalainen, A.; Alapuranen, M.; Kallio, J.; Hooman, S.; Viikari, L.; Vehmaanperae, J.; Koivula, A.
Biotechnol. Bioeng.
101
515-528
Cel9D, an atypical 1,4-beta-D-glucan glucohydrolase from Fibrobacter succinogenes: characteristics, catalytic residues, and synergistic interactions with other cellulases
2008
Qi, M.; Jun, H.S.; Forsberg, C.W.
J. Bacteriol.
190
1976-1984
Purification and characterization of a thermostable xylanase from the brown-rot fungus Laetiporus sulphureus
2009
Lee, J.W.; Park, J.Y.; Kwon, M.; Choi, I.G.
J. Biosci. Bioeng.
107
33-37
Cloning, sequencing, and expression of the gene encoding a multidomain endo-beta-1,4-Xylanase from Paenibacillus curdlanolyticus B-6, and characterization of the recombinant enzyme
2009
Waeonukul, R.; Pason, P.; Kyu, K.L.; Sakka, K.; Kosugi, A.; Mori, Y.; Ratanakhanokchai, K.
J. Microbiol. Biotechnol.
19
277-285
Characterization of beta-glucosidase from a strain of Penicillium purpurogenum KJS506
2010
Jeya, M.; Joo, A.R.; Lee, K.M.; Tiwari, M.K.; Lee, K.M.; Kim, S.H.; Lee, J.K.
Appl. Microbiol. Biotechnol.
86
1473-1484
Mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion (AFEX) pretreated corn stover
2010
Gao, D.; Chundawat, S.P.; Krishnan, C.; Balan, V.; Dale, B.E.
Biores. Technol.
101
2770-2781
Purification, characterization and sequencing of the major beta-1,3-glucanase from the midgut of Tenebrio molitor larvae
2009
Genta, F.A.; Bragatto, I.; Terra, W.R.; Ferreira, C.
Insect Biochem. Mol. Biol.
39
861-874
SCHEMA recombination of a fungal cellulase uncovers a single mutation that contributes markedly to stability
2009
Heinzelman, P.; Snow, C.D.; Smith, M.A.; Yu, X.; Kannan, A.; Boulware, K.; Villalobos, A.; Govindarajan, S.; Minshull, J.; Arnold, F.H.
J. Biol. Chem.
284
26229-26233
Functional analysis of conserved aromatic amino acids in the discoidin domain of Paenibacillus beta-1,3-glucanase
2009
Cheng, Y.; Hsieh, F.; Meng, M.
Microb. Cell Fact.
8
62
Production and characterization of cellobiohydrolase from a novel strain of Penicillium purpurogenum KJS506
2011
Lee, K.M.; Joo, A.R.; Jeya, M.; Lee, K.M.; Moon, H.J.; Lee, J.K.
Appl. Biochem. Biotechnol.
163
25-39
Characterization of a cellobiohydrolase (MoCel6A) produced by Magnaporthe oryzae
2010
Takahashi, M.; Takahashi, H.; Nakano, Y.; Konishi, T.; Terauchi, R.; Takeda, T.
Appl. Environ. Microbiol.
76
6583-6590
Enzymatic hydrolysis of cellulose by the cellobiohydrolase domain of CelB from the hyperthermophilic bacterium Caldicellulosiruptor saccharolyticus
2011
Park, J.I.; Kent, M.S.; Datta, S.; Holmes, B.M.; Huang, Z.; Simmons, B.A.; Sale, K.L.; Sapra, R.
Biores. Technol.
102
5988-5994
Mechanism of initial rapid rate retardation in cellobiohydrolase catalyzed cellulose hydrolysis
2010
Jalak, J.; Vaeljamaee, P.
Biotechnol. Bioeng.
106
871-883
Molecular cloning, purification and characterization of thermostable beta-1,3-1,4 glucanase from Bacillus subtilis A8-8
2010
Jung, Y.J.; Lee, Y.S.; Park, I.H.; Chandra, M.S.; Kim, K.K.; Choi, Y.L.
Indian J. Biochem. Biophys.
47
203-210
Purification and characterization of a thermostable cellobiohydrolase from Fomitopsis pinicola
2010
Shin, K.; Kim, Y.H.; Jeya, M.; Lee, J.K.; Kim, Y.S.
J. Microbiol. Biotechnol.
20
1681-1688
-
Screening and characterization of a cellulase with endocellulase and exocellulase activity from yak rumen metagenome
2011
Bao, L.; Huang, Q.; Chang, L.; Zhou, J.; Lu, H.
J. Mol. Catal. B
73
104-110
Substrate-binding properties of family 54 module of laminarinase Lic16A of Clostridium thermocellum
2010
Dvortsov, I.; Lunina, N.; Zverlov, V.; Velikodvorskaia, G.
Mol. Biol. (Mosk.)
44
671-676
Hyperthermostable endoglucanase from Pyrococcus horikoshii
2002
Ando, S.; Ishida, H.; Kosugi, Y.; Ishikawa, K.
Appl. Environ. Microbiol.
68
430-433
Complete saccharification of cellulose at high temperature using endocellulase and beta-glucosidase from Pyrococcus sp.
2010
Kim, H.W.; Ishikawa, K.
J. Microbiol. Biotechnol.
20
889-892
Purification and characterization of a thermophilic cellulase from a novel cellulolytic strain, Paenibacillus barcinonensis
2012
Asha, B.M.; Revathi, M.; Yadav, A.; Sakthivel, N.
J. Microbiol. Biotechnol.
22
1501-1509
Transient kinetics and rate-limiting steps for the processive cellobiohydrolase Cel7A: effects of substrate structure and carbohydrate binding domain
2013
Cruys-Bagger, N.; Tatsumi, H.; Ren, G.R.; Borch, K.; Westh, P.
Biochemistry
52
8938-8948
Role of cysteine residues in thermal inactivation of fungal Cel6A cellobiohydrolases
2013
Wu, I.; Heel, T.; Arnold, F.H.
Biochim. Biophys. Acta
1834
1539-1544
Xylo-oligosaccharides are competitive inhibitors of cellobiohydrolase I from Thermoascus aurantiacus
2012
Zhang, J.; Viikari, L.
Biores. Technol.
117
286-291
Effect of temperature on lignin-derived inhibition studied with three structurally different cellobiohydrolases
2013
Rahikainen, J.L.; Moilanen, U.; Nurmi-Rantala, S.; Lappas, A.; Koivula, A.; Viikari, L.; Kruus, K.
Biores. Technol.
146
118-125
Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures
2013
Wu, I.; Arnold, F.H.
Biotechnol. Bioeng.
110
1874-1883
Joint X-ray crystallographic and molecular dynamics study of cellobiohydrolase I from Trichoderma harzianum: deciphering the structural features of cellobiohydrolase catalytic activity
2013
Textor, L.C.; Colussi, F.; Silveira, R.L.; Serpa, V.; de Mello, B.L.; Muniz, J.R.; Squina, F.M.; Pereira, N.; Skaf, M.S.; Polikarpov, I.
FEBS J.
280
56-69
Structural characterization of a unique marine animal family 7 cellobiohydrolase suggests a mechanism of cellulase salt tolerance
2013
Kern, M.; McGeehan, J.E.; Streeter, S.D.; Martin, R.N.; Besser, K.; Elias, L.; Eborall, W.; Malyon, G.P.; Payne, C.M.; Himmel, M.E.; Schnorr, K.; Beckham, G.T.; Cragg, S.M.; Bruce, N.C.; McQueen-Mason, S.J.
Proc. Natl. Acad. Sci. USA
110
10189-10194
Cloning and characterization of a thermostable and pH-stable cellobiohydrolase from Neocallimastix patriciarum J11
2013
Wang, H.C.; Chen, Y.C.; Huang, C.T.; Hseu, R.S.
Protein Expr. Purif.
90
153-159
-
Kinetic behavior of free and in situ immobilized chitosanases produced by the fungus Mucor rouxii
2014
Abdel-Aziz, S.; Kahil, T.; Keera, A.
World Appl. Sci. J.
30
1-9
Biochemical and structural characterization of two Dictyostelium cellobiohydrolases from the Amoebozoa kingdom reveal a high conservation between distant phylogenetic trees of life
2016
Hobdey, S.E.; Knott, B.C.; Haddad Momeni, M.; Taylor, L.E.; Borisova, A.S.; Podkaminer, K.K.; VanderWall, T.A.; Himmel, M.E.; Decker, S.R.; Beckham, G.T.; Stahlberg, J.
Appl. Environ. Microbiol.
82
3395-3409
Cloning and characterization of an endoglucanase gene from Actinomyces sp. Korean Native Goat 40
2016
Kim, S.C.; Kang, S.H.; Choi, E.Y.; Hong, Y.H.; Bok, J.D.; Kim, J.Y.; Lee, S.S.; Choi, Y.J.; Choi, I.S.; Cho, K.K.
Asian-Australas. J. Anim. Sci.
29
126-133
Production and characterization of highly thermostable beta-glucosidase during the biodegradation of methyl cellulose by Fusarium oxysporum
2016
Olajuyigbe, F.; Nlekerem, C.; Ogunyewo, O.
Biochem. Res. Int.
2016
3978124
Engineered thermostable fungal cellulases exhibit efficient synergistic cellulose hydrolysis at elevated temperatures
2014
Trudeau, D.L.; Lee, T.M.; Arnold, F.H.
Biotechnol. Bioeng.
111
2390-2397
Mechanism of product inhibition for cellobiohydrolase Cel7A during hydrolysis of insoluble cellulose
2016
Olsen, J.P.; Alasepp, K.; Kari, J.; Cruys-Bagger, N.; Borch, K.; Westh, P.
Biotechnol. Bioeng.
113
1178-1186
N-linked glycosylation of recombinant cellobiohydrolase I (Cel7A) from Penicillium verruculosum and its effect on the enzyme activity
2016
Dotsenko, A.S.; Gusakov, A.V.; Volkov, P.V.; Rozhkova, A.M.; Sinitsyn, A.P.
Biotechnol. Bioeng.
113
283-291
Sequencing, biochemical characterization, crystal structure and molecular dynamics of cellobiohydrolase Cel7A from Geotrichum candidum 3C
2015
Borisova, A.S.; Eneyskaya, E.V.; Bobrov, K.S.; Jana, S.; Logachev, A.; Polev, D.E.; Lapidus, A.L.; Ibatullin, F.M.; Saleem, U.; Sandgren, M.; Payne, C.M.; Kulminskaya, A.A.; Stahlberg, J.
FEBS J.
282
4515-4537
Synergistic function of four novel thermostable glycoside hydrolases from a long-term enriched thermophilic methanogenic digester
2015
Wang, M.; Lai, G.L.; Nie, Y.; Geng, S.; Liu, L.; Zhu, B.; Shi, Z.; Wu, X.L.
Front. Microbiol.
6
509
Characterization of a novel endoglucanase from Ganoderma lucidum
2015
Manavalan, T.; Manavalan, A.; Thangavelu, K.P.; Heese, K.
J. Basic Microbiol.
55
761-771
Temperature effects on kinetic parameters and substrate affinity of Cel7A cellobiohydrolases
2015
Sorensen, T.H.; Cruys-Bagger, N.; Windahl, M.S.; Badino, S.F.; Borch, K.; Westh, P.
J. Biol. Chem.
290
22193-22202
Improving Trichoderma reesei Cel7B thermostability by targeting the weak spots
2014
Zhang, S.; Wang, Y.; Song, X.; Hong, J.; Zhang, Y.; Yao, L.
J. Chem. Inf. Model.
54
2826-2833
Recombinant expression and characterization of a novel endoglucanase from Bacillus subtilis in Escherichia coli
2014
Zafar, M.; Ahmed, S.; Khan, M.I.; Jamil, A.
Mol. Biol. Rep.
41
3295-3302
First thermostable endo-beta-1,4-glucanase from newly isolated Xanthomonas sp. EC102
2014
Woo, M.H.; Chang, Y.H.; Lee, H.S.; Pak, P.J.; Kim, J.S.; Chung, N.
Protein J.
33
110-117
Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessing
2014
Wei, H.; Wang, W.; Alahuhta, M.; Vander, Wall, T.; Baker, J.O.; Taylor, L.E. 2nd; Decker, S.R.; Himmel, M.E.; Zhang, M.
Biotechnol. Biofuels
7
148
Antagonistic activity of endo-beta-1,3-glucanase from a novel isolate, Streptomyces sp. 9X166, against black rot in orchids
2016
Sakdapetsiri, C.; Fukuta, Y.; Aramsirirujiwet, Y.; Shirasaka, N.; Kitpreechavanich, V.
J. Basic Microbiol.
56
469-479
The immunoreactive exo-1,3-beta-glucanase from the pathogenic oomycete Pythium insidiosum is temperature regulated and exhibits glycoside hydrolase activity
2015
Keeratijarut, A.; Lohnoo, T.; Rujirawat, T.; Yingyong, W.; Kalambaheti, T.; Miller, S.; Phuntumart, V.; Krajaejun, T.
PLoS ONE
10
e0135239
Two neutral thermostable cellulases from Phialophora sp. G5 act synergistically in the hydrolysis of filter paper
2012
Zhao, J.; Shi, P.; Li, Z.; Yang, P.; Luo, H., Bai, Y., Wang, Y.; Yao, B.
Bioresour. Technol.
121
404-410
Isolation of four major subunits from Clostridium thermocellum cellulosome and their synergism in the hydrolysis of crystalline cellulose
1994
Bhat, S.; Goodenough, P.W.; Bhat, M.K.; Owen, E.
Int. J. Biol. Macromol.
16
335-342
A C4-oxidizing lytic polysaccharide monooxygenase cleaving both cellulose and cello-oligosaccharides
2014
Isaksen, T.; Westereng, B.; Aachmann, F.L.; Agger, J.W.; Kracher, D.; Kittl, R.; Ludwig, R.; Haltrich, D.; Eijsink, V.G.; Horn, S.J.
J. Biol. Chem.
289
2632-2642
Cellulose surface degradation by a lytic polysaccharide monooxygenase and its effect on cellulase hydrolytic efficiency
2014
Eibinger, M.; Ganner, T.; Bubner, P.; Rosker, S.; Kracher, D.; Haltrich, D.; Ludwig, R.; Plank, H.; Nidetzky, B.
J. Biol. Chem.
289
35929-35938
Enhanced polysaccharide binding and activity on linear beta-glucans through addition of carbohydrate-binding modules to either terminus of a glucooligosaccharide oxidase
2015
Foumani, M.; Vuong, T.V.; MacCormick, B.; Master, E.R.
PLoS ONE
10
e0125398
Structural and functional characterization of a conserved pair of bacterial cellulose-oxidizing lytic polysaccharide monooxygenases
2014
Forsberg, Z.; Mackenzie, A.; Sorlie, M.; Rohr, A.; Helland, R.; Arvai, A.; Vaaje-Kolstad, G.; Eijsink, V.
Proc. Natl. Acad. Sci. USA
111
8446-8451
-
Characterisation of a chimeric Phanerochaete chrysosporium cellobiohydrolase expressed from Escherichia coli
2004
Howard, R.; Masoko, P.; Mowa, M.; Abotsi, E.; Howard, S.
Afr. J. Biotechnol.
3
349-352
-
Production and purification of thermostable cellulases from Humicola insolens YH-8
1980
Hayashida, S.; Yoshioka, H.
Agric. Biol. Chem.
44
1721-1728
Substrate specificity and mode of action of the cellulases from the thermophilic fungus Thermoascus aurantiacus
1981
Shepherd, M.G.; Tong, C.C.; Cole, A.L.
Biochem. J.
193
67-74
-
Purification and characterization of an endocellulase from the thermophilic fungus Chaetomium thermophilum CT2
2003
Li, D.; Lu, M.; Li, Y.; Lu, J.
Enzyme Microb. Technol.
33
932-937
Purification and biochemical characterization of glucose-cellobiose-tolerant cellulases from Scytalidium thermophilum
2013
Silva, J.C.; Guimaraes, L.H.; Salgado, J.C.; Furriel, R.P.; Polizeli, M.L.; Rosa, J.C.; Jorge, J.A.
Folia Microbiol. (Praha)
58
561-568
-
Cloning of a gene encoding thermostable cellobiohydrolase from the thermophilic fungus Chaetomium thermophilum and its expression in Pichia pastoris
2009
Li, Y.; Li, H.; Li, A.; Li, D.
J. Appl. Microbiol.
106
1867-1875
Thermophilic fungi as new sources for production of cellulases and xylanases with potential use in sugarcane bagasse saccharification
2015
de Cassia Pereira, J.; Paganini Marques, N.; Rodrigues, A.; Brito de Oliveira, T.; Boscolo, M.; da Silva, R.; Gomes, E.; Bocchini Martins, D.A.
J. Appl. Microbiol.
118
928-939
Expression of Talaromyces emersonii cellobiohydrolase Cel7A in Saccharomyces cerevisiae and rational mutagenesis to improve its thermostability and activity
2010
Voutilainen, S.; Murray, P.; Tuohy, M.; Koivula, A.
Protein Eng. Des. Sel.
23
69-79
Engineering ionic liquid-tolerant cellulases for biofuels production
2016
Wolski, P.; Dana, C.; Clark, D.; Blanch, H.
Protein Eng. Des. Sel.
29
117-122
-
Improving the hydrolytic action of cellulases by Tween 80 offsetting the lost activity of cellobiohydrolase Cel7A
2017
Xin, D.; Yang, M.; Chen, X.; Zhang, Y.; Ma, L.; Zhang, J.
ACS Sust. Chem. Eng.
5
11339-11345
Genetic and functional characterization of an extracellular modular GH6 endo-beta-1,4-glucanase from an earthworm symbiont, Cellulosimicrobium funkei HY-13
2016
Kim, D.; Lee, M.; Cho, H.; Lee, J.; Lee, M.; Chung, C.; Shin, D.; Rhee, Y.; Son, K.; Park, H.
Antonie van Leeuwenhoek
109
1-12
Expression and characteristics of an endoglucanase from Trichoderma atroviride (TaEGII) in Saccharomyces cerevisiae
2017
Huang, X.M.; Li, Q.Q.; Chen, X.L.; Fan, J.X.; Xu, X.H.; Sun, X.D.; Li, D.Y.; Zhao, H.X.
Appl. Biochem. Biotechnol.
182
1158-1170
Biochemical and structural characterizations of two Dictyostelium cellobiohydrolases from the amoebozoa kingdom reveal a high level of conservation between distant phylogenetic trees of life
2016
Hobdey, S.E.; Knott, B.C.; Haddad Momeni, M.; Taylor, L.E.; Borisova, A.S.; Podkaminer, K.K.; VanderWall, T.A.; Himmel, M.E.; Decker, S.R.; Beckham, G.T.; Stahlberg, J.
Appl. Environ. Microbiol.
82
3395-3409
Functional analysis of hyperthermophilic endocellulase from Pyrococcus horikoshii by crystallographic snapshots
2011
Kim, H.; Ishikawa, K.
Biochem. J.
437
223-230
Direct kinetic comparison of the two cellobiohydrolases Cel6A and Cel7A from Hypocrea jecorina
2017
Badino, S.F.; Kari, J.; Christensen, S.J.; Borch, K.; Westh, P.
Biochim. Biophys. Acta
1865
1739-1745
N-linked glycosylation of recombinant cellobiohydrolase I (Cel7A) from Penicillium verruculosum and its effect on the enzyme activity
2016
Dotsenko, A.; Gusakov, A.; Volkov, P.; Rozhkova, A.; Sinitsyn, A.
Biotechnol. Bioeng.
113
283-291
Fast purification method of functional LPMOs from Streptomyces ambofaciens by affinity adsorption
2017
Valenzuela, S.; Ferreres, G.; Margalef, G.; Pastor, F.
Carbohydr. Res.
448
205-211
A comparative study on the activity of fungal lytic polysaccharide monooxygenases for the depolymerization of cellulose in soybean spent flakes
2017
Pierce, B.; Agger, J.; Zhang, Z.; Wichmann, J.; Meyer, A.
Carbohydr. Res.
449
85-94
Biophysical and biochemical studies of a major endoglucanase secreted by Xanthomonas campestris pv. campestris
2016
Rosseto, F.R.; Manzine, L.R.; de Oliveira Neto, M.; Polikarpov, I.
Enzyme Microb. Technol.
91
1-7
Biochemical and structural insights into a thermostable cellobiohydrolase from Myceliophthora thermophila
2018
Kadowaki, M.A.S.; Higasi, P.; de Godoy, M.O.; Prade, R.A.; Polikarpov, I.
FEBS J.
285
559-579
-
Ionic liquid tolerant hyperthermophilic cellulases for biomass pretreatment and hydrolysis
2010
Datta, S.; Holmes, B.; Park, J.; Chen, Z.; Dibble, D.; Hadi, M.; Blanch, H.; Simmons, B.; Sapra, R.
Green Chem.
12
338-345
Antagonistic activity of endo-beta-1,3-glucanase from a novel isolate, Streptomyces sp. 9X166, against black rot in orchids
2016
Sakdapetsiri, C.; Fukuta, Y.; Aramsirirujiwet, Y.; Shirasaka, N.; Kitpreechavanich, V.
J. Basic Microbiol.
56
469-479
Expression and characterization of the processive exo-beta-1,4-cellobiohydrolase SCO6546 from Streptomyces coelicolor A(3)
2018
Lee, C.R.; Chi, W.J.; Lim, J.H.; Dhakshnamoorthy, V.; Hong, S.K.
J. Basic Microbiol.
58
310-321
Inter-domain synergism is required for efficient feeding of cellulose chain into active site of cellobiohydrolase Cel7A
2016
Kont, R.; Kari, J.; Borch, K.; Westh, P.; Vaeljamaee, P.
J. Biol. Chem.
291
26013-26023
The influence of different linker modifications on the catalytic activity and cellulose affinity of cellobiohydrolase Cel7A from Hypocrea jecorina
2017
Badino, S.F.; Bathke, J.K.; S?rensen, T.H.; Windahl, M.S.; Jensen, K.; Peters, G.H.J.; Borch, K.; Westh, P.
Protein Eng. Des. Sel.
30
495-501
Production and characteristics of avicel-digesting and non-avicel-digesting cellobiohydrolases from Aspergillus ficum
1988
Hayashida, S.; Mo, K.; Hosoda, A.
Appl. Environ. Microbiol.
54
1523-1529
Kinetic parameters and mode of action of the cellobiohydrolases produced by Talaromyces emersonii
2002
Tuohy, M.G.; Walsh, D.J.; Murray, P.G.; Claeyssens, M.; Cuffe, M.M.; Savage, A.V.; Coughlan, M.P.
Biochim. Biophys. Acta
1596
366-380
Hydrolysis of microcrystalline cellulose by cellobiohydrolase I and endoglucanase II from Trichoderma reesei Adsorption, sugar production pattern, and synergism of the enzymes
1998
Medve, J.; Karlsson, J.; Lee, D.; Tjerneld, F.
Biotechnol. Bioeng.
59
621-634
A functionally based model for hydrolysis of cellulose by fungal cellulase
2006
Zhang, Y.H.; Lynd, L.R.
Biotechnol. Bioeng.
94
888-898
Enhanced enzymatic cellulose degradation by cellobiohydrolases via product removal
2013
Gavlighi, H.A.; Meyer, A.S.; Mikkelsen, J.D.
Biotechnol. Lett.
35
205-212
The cellulose binding region in Trichoderma reesei cellobiohydrolase I has a higher capacity in improving crystalline cellulose degradation than that of Penicillium oxalicum
2018
Du, J.; Zhang, X.; Li, X.; Zhao, J.; Liu, G.; Gao, B.; Qu, Y.
Biores. Technol.
266
19-25
Expression of an endoglucanase-cellobiohydrolase fusion protein in Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi
2018
Xu, Q.; Alahuhta, M.; Wei, H.; Knoshaug, E.P.; Wang, W.; Baker, J.O.; Vander Wall, T.; Himmel, M.E.; Zhang, M.
Biotechnol. Biofuels
11
322
Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum
2018
Liu, Y.J.; Liu, S.; Dong, S.; Li, R.; Feng, Y.; Cui, Q.
Biotechnol. Biofuels
11
6-6
Replacement of carbohydrate binding modules improves acetyl xylan esterase activity and its synergistic hydrolysis of different substrates with xylanase
2016
Liu, S.; Ding, S.
BMC Biotechnol.
16
73
Functional characterization and comparative analysis of two heterologous endoglucanases from diverging subfamilies of glycosyl hydrolase family 45
2019
Berto, G.L.; Velasco, J.; Tasso Cabos Ribeiro, C.; Zanphorlin, L.M.; Noronha Domingues, M.; Tyago Murakami, M.; Polikarpov, I.; de Oliveira, L.C.; Ferraz, A.; Segato, F.
Enzyme Microb. Technol.
120
23-35
Rate-limiting step and substrate accessibility of cellobiohydrolase Cel6A from Trichoderma reesei
2018
Christensen, S.J.; Kari, J.; Badino, S.F.; Borch, K.; Westh, P.
FEBS J.
285
4482-4493
Bi-functional fusion enzyme (EG-M-Xyn)EG-M-Xyn displaying endoglucanase and xylanase activities and its utility in improving lignocellulose degradation
2018
Chen, C.C.; Gao, G.J.; Kao, A.L.; Tsai, Z.C.
Int. J. Biol. Macromol.
111
722-729
Systematic deletions in the cellobiohydrolase (CBH) Cel7A from the fungus Trichoderma reesei reveal flexible loops critical for CBH activity
2019
Schiano-di-Cola, C.; Rojel, N.; Jensen, K.; Kari, J.; Sorensen, T.H.; Borch, K.; Westh, P.
J. Biol. Chem.
294
1807-1815
Enzymatic characteristics of a highly thermostable beta-(1-4)-glucanase from Fervidobacterium islandicum AW-1 (KCTC 4680)
2017
Jeong, W.S.; Seo, D.H.; Jung, J.H.; Jung, D.H.; Lee, D.W.; Park, Y.S.; Park, C.
J. Microbiol. Biotechnol.
27
271-276
Discovery and characterization of a thermostable two-domain GH6 endoglucanase from a compost metagenome
2018
Jensen, M.S.; Fredriksen, L.; MacKenzie, A.K.; Pope, P.B.; Leiros, I.; Chylenski, P.; Williamson, A.K.; Christopeit, T.; Ostby, H.; Vaaje-Kolstad, G.; Eijsink, V.G.H.
PLoS ONE
13
e0197862
Construction of Escherichia coli BL21/A-53 producing histidine-tagged carboxymethylcellulase and comparison of its characteristics with CMCase without histidine-tag
2019
Kang, D.U.; Lee, Y.S.; Lee, J.W.
Prep. Biochem. Biotechnol.
49
167-175
Construction of thermostable cellobiohydrolase I from the fungus Talaromyces cellulolyticus by protein engineering
2019
Nakabayashi, M.; Kamachi, S.; Malle, D.; Yanamoto, T.; Kishishita, S.; Fujii, T.; Inoue, H.; Ishikawa, K.
Protein Eng. Des. Sel.
32
33-40
Functional characterisation of cellobiohydrolase I (Cbh1) from Trichoderma virens UKM1 expressed in Aspergillus niger
2019
Wahab, A.F.F.A.; Abdul Karim, N.A.; Ling, J.G.; Hasan, N.S.; Yong, H.Y.; Bharudin, I.; Kamaruddin, S.; Abu Bakar, F.D.; Murad, A.M.A.
Protein Expr. Purif.
154
52-61
Competitive inhibition of cellobiohydrolase I by manno-oligosaccharides
2015
Xin, D.; Ge, X.; Sun, Z.; Viikari, L.; Zhang, J.
Enzyme Microb. Technol.
68
62-68
Links to other databases for Avicel
ChEBI
PubChem
-
-
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
Server: brenda6