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Ligand 5beta-pregnane-21-ol-3,20-dione

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Substrate (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.270

5beta-pregnane-21-ol-3,20-dione + NADPH + H+ = 5beta-pregnane-3beta,21-diol-20-one + NADP+

724124

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1.3.99.6

21-hydroxy-5beta-pregnan-3,20-dione + acceptor = 21-hydroxy-pregn-4-en-3,20-dione + reduced acceptor

391402

-

Product in Enzyme-catalyzed Reactions (3 results)

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Show Product (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.239

5beta-pregnan-3alpha,21-diol-20-one + NAD+ = 5beta-pregnan-21-ol-3,20-dione + NADH

286354

-

1.1.1.270

5beta-pregnane-3beta,21-diol-20-one + NADP+ = 5beta-pregnane-21-ol-3,20-dione + NADPH + H+

-

-

1.1.1.50

3alpha,21-dihydroxy-5beta-pregnan-20-one + NADP+ = 21-hydroxy-5beta-pregnan-3,20-dione + NADPH

-

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (5 results)

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Show Turnover Number (5 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.1.1.270

0.023

-

pH 7.4, 25°C, mutant F303Q/M304S

724124

1.1.1.270

0.042

-

pH 7.4, 25°C, mutant M304S

724124

1.1.1.270

0.062

-

pH 7.4, 25°C, mutant F303Q

724124

1.1.1.270

0.063

-

pH 7.4, 25°C, mutant L116F

724124

1.1.1.270

0.092

-

pH 7.4, 25°C, wild-type enzyme

724124

1.1.1.270

5 entries

K_M Value (5 results)

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Show KM Value (5 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.1.270

0.0023

-

pH 7.4, 25°C, mutant L116F

724124

1.1.1.270

0.0024

-

pH 7.4, 25°C, wild-type enzyme

724124

1.1.1.270

0.013

-

pH 7.4, 25°C, mutant F303Q

724124

1.1.1.270

0.023

-

pH 7.4, 25°C, mutant M304S

724124

1.1.1.270

0.05

-

pH 7.4, 25°C, mutant F303Q/M304S

724124

1.1.1.270

5 entries

References & Links

Literature References (3)

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Show Reference (3 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

286354

2317205

Demonstration of 3alpha(17beta)-hydroxysteroid dehydrogenase distinct from 3alpha-hydroxysteroid dehydrogenase in hamster liver

1990

Ohmura, M.; Hara, A.; Nakagawa, M.; Sawada, H.

Biochem. J.

266

583-589

391402

5907467

Purification and mechanism of action of a steroid delta-4-5-beta-dehydrogenase

1966

Davidson, S.J.; Talalay, P.

J. Biol. Chem.

241

906-915

724124

22874434

Characterization of rabbit aldose reductase-like protein with 3beta-hydroxysteroid dehydrogenase activity

2012

Endo, S.; Matsunaga, T.; Kumada, S.; Fujimoto, A.; Ohno, S.; El-Kabbani, O.; Hu, D.; Toyooka, N.; Mano, J.; Tajima, K.; Hara, A.

Arch. Biochem. Biophys.

527

23-30

Links to other databases for 5beta-pregnane-21-ol-3,20-dione

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