Ligand 10-hydroxy-alpha-humulene
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Basic Ligand Information
Molecular Structure

C15H24O
10-hydroxy-alpha-humulene
NLCQRJBYGGWZRQ-SKTNYSRSSA-N
(2E,6E,10E)-2,6,9,9-tetramethylcycloundeca-2,6,10-trien-1-ol
Roles as Enzyme Ligand
In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)
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10-hydroxy-alpha-humulene + NAD+ = zerumbone + NADH + H+
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In Vivo Product in Enzyme-catalyzed Reactions (1 result)
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alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase] = 10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
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Substrate in Enzyme-catalyzed Reactions (1 result)
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10-hydroxy-alpha-humulene + NAD+ = zerumbone + NADH + H+
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Product in Enzyme-catalyzed Reactions (1 result)
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alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase] = 10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (4 results)
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0.1
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recombinant mutant S142A, pH 8.0, 37°C
0.1
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recombinant mutant Y155A, pH 8.0, 37°C
1.3
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recombinant wild-type enzyme, pH 8.0, 37°C
2.9
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recombinant mutant S144A, pH 8.0, 37°C
KM Value (4 results)
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0.028
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recombinant mutant S144A, pH 8.0, 37°C
0.0585
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recombinant wild-type enzyme, pH 8.0, 37°C
0.0782
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recombinant mutant Y155A, pH 8.0, 37°C
0.138
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recombinant mutant S142A, pH 8.0, 37°C
References & Links
Literature References (2)
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A short-chain dehydrogenase involved in terpene metabolism from Zingiber zerumbet
2011
Okamoto, S.; Yu, F.; Harada, H.; Okajima, T.; Hattan, J.; Misawa, N.; Utsumi, R.
FEBS J.
278
2892-2900
Production of sesquiterpenoid zerumbone from metabolic engineered Saccharomyces cerevisiae
2018
Zhang, C.; Liu, J.; Zhao, F.; Lu, C.; Zhao, G.R.; Lu, W.
Metab. Eng.
49
28-35
Links to other databases for 10-hydroxy-alpha-humulene