Ligand tert-butyl hydroperoxide

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Basic Ligand Information

Molecular Structure
Picture of tert-butyl hydroperoxide (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C4H10O2
tert-butyl hydroperoxide
CIHOLLKRGTVIJN-UHFFFAOYSA-N
Synonyms:
t-butyl hydroperoxide, tert-butyl-hydroperoxide, tert-butylhydroperoxide, tertbutyl hydroperoxide, tertiary butyl hydroperoxide

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
tert-butyl hydroperoxide + 2 GSH = tert-butyl alcohol + GSSG + H2O
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (108 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
tert-butyl hydroperoxide + NADPH + H+ = ? + NADP+ + H2O
show the reaction diagram
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tert-butyl hydroperoxide + NADPH + H+ = ?
show the reaction diagram
-
thioredoxin + tert-butyl-hydroperoxide = ?
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
stimulation up to 100 mM
-
0.5 mM, oxidative stress induces Pseudomonas putida FprA but not FprB
-
tert-butyl hydroperoxide significantly increases the sulfation of 7-hydroxycoumarin at an optimal concentration of 0.01 mM
-
activation is inhibited by MG132
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Inhibitor in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
irreversible, inactivation is associated with -SH group oxidation
-
1% residual activity after treatment with 290 mM tert-butyl hydroperoxide at 50°C and pH 7 for 3 h
-
kinetic studies
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substrate inhibition
-
0.01 mM
-
1% residual activity after treatment with 290 mM tert-butyl hydroperoxide at 50°C and pH 7 for 3 h
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (15 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
1.5
-
-
320
-
in 50 mM potassium phosphate buffer, pH 7.0
25.3
-
27°C, pH 7.0, activity of seleno-glutathione transferase

KM Value (34 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
22
-
in 50 mM potassium phosphate buffer, pH 7.0

References & Links

Links to other databases for tert-butyl hydroperoxide

ChEBI
PubChem
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PubChem