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0.1
-
pH 7.0, 30°C, mutant enzyme L239M/T245A
0.2
-
pH 7.0, 30°C, mutant enzyme F236V
1.3
-
pH 7.0, 30°C, mutant enzyme T245A
9.5
-
pH 7.0, 30°C, mutant enzyme F236S
20
-
pH 7.0, 30°C, mutant enzyme L239F
22
-
pH 7.0, 30°C, mutant enzyme L239W
92
-
pH 7.0, 30°C, mutant enzyme G234V/G235D
150
-
pH 7.0, 30°C, mutant enzyme L239M
520
-
pH 7.0, 30°C, mutant enzyme L239A
18000
-
pH 7.0, 30°C, wild-type enzyme
0.833
-
pH 6.9, 85°C, under argon
1.33
-
pH 6.9, 85°C, under argon
0.06
-
mutant T45M, pH 9.5, 35°C
0.101
-
mutant E116V, pH 9.5, 35°C
0.182
-
wild-type, pH 9.5, 35°C
0.214
-
mutant T45M/E116V, pH 9.5, 35°C
1.2
-
mutant I472A/I476F, pH 6.5, 30°C
6.9
-
mutant I472A, pH 6.5, 30°C
0.07
-
pH 7.0, mutant enzyme H70A
0.46
-
mutant enzyme H70A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.46
-
pH and temperature not specified in the publication, mutant H70A
1.41
-
pH 7.0, mutant enzyme H281A
2
-
mutant enzyme H281T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
2.1
-
mutant enzyme H281A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
2.1
-
pH and temperature not specified in the publication, mutant H281A
4.45
-
pH 7.0, mutant enzyme S26A
4.5
-
mutant enzyme H70F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
4.5
-
pH and temperature not specified in the publication, mutant H70F
4.6
-
mutant enzyme I466A, at pH 6.0 and 30°C
5.1
-
wild type enzyme, at pH 6.0 and 30°C
5.6
-
mutant enzyme H70S, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
6.9
-
mutant enzyme H281Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
6.9
-
pH and temperature not specified in the publication, mutant H281Y
8.6
-
mutant enzyme H281Q, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
14
-
mutant enzyme H70L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
14
-
pH and temperature not specified in the publication, mutant H70L
15
-
mutant enzyme S26A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
15
-
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
15
-
pH and temperature not specified in the publication, mutant S26A
17
-
mutant enzyme H281W, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
19
-
mutant enzyme H281N in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
21
-
pH 6.0, 30°C, mutant enzyme A460I/F464I
26
-
mutant enzyme S26M, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
26
-
pH and temperature not specified in the publication, mutant S26M
42
-
mutant enzyme H70T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
62
-
pH 6.0, 30°C, mutant enzyme A460I
65
-
mutant enzyme H281F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
65
-
pH and temperature not specified in the publication, mutant H281F
94
-
mutant enzyme S26T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
123
-
pH 6.0, 30°C, mutant enzyme F464I
132
-
mutant enzyme S26L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
132
-
pH and temperature not specified in the publication, mutant S26L
222
-
wild type enzyme at pH 6.0 and 35°C
241
-
pH 7.0, wild-type enzyme
284
-
recombinant, his-tagged enzyme at pH 6.0 and 35°C
320
-
wild type enzyme, at pH 6.0 and 30°C
320
-
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
320
-
pH and temperature not specified in the publication, wild-type enzyme
426
-
at 30°C, in 50 mM potassium phosphate buffer, pH 6.5
1349
-
pH 6.0, 30°C, wild-type enzyme
0.01
-
mutant D29E, pH 6.5, 30°C
0.07
-
mutant E468D, pH 6.5, 30°C
0.29
-
mutant H115K, pH 6.5, 30°C
0.87
-
mutant E52D, pH 6.5, 30°C
2.25
-
mutant Q383T, pH 6.5, 30°C
11.6
-
wild-type, pH 6.5, 30°C
31.4
-
at pH 6.5, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
37.55
-
enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde
0.26
-
pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2
1.5
-
at pH 7.0 and 37°C
0.466
-
pH 6.9, 85°C, under argon
0.6
-
pH 6.9, 85°C, under argon
0.75
-
mutant E116V, pH 9.5, 35°C
1.12
-
mutant T45M/E116V, pH 9.5, 35°C
1.99
-
mutant T45M, pH 9.5, 35°C
9.38
-
wild-type, pH 9.5, 35°C
1.8
-
mutant I472A, pH 6.5, 30°C
4.4
-
mutant I472A/I476F, pH 6.5, 30°C
0.06
-
mutant enzyme L461V, in potassium phosphate buffer (pH 6.5, 50 mM)
0.2
-
mutant enzyme A460I
0.2
-
pH 6.0, 30°C, mutant enzyme A460I
0.23
-
in 50 mM potassium phosphate buffer, pH 6.5, at 30°C
0.25
-
mutant enzyme L461A, in potassium phosphate buffer (pH 6.5, 50 mM)
0.27
-
wild type enzyme, at pH 6.0 and 30°C
0.27
-
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.27
-
pH and temperature not specified in the publication, wild-type enzyme
0.29
-
mutant enzyme H281Q, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.29
-
mutant enzyme H281W, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.37
-
pH 7.0, wild-type enzyme
0.37
-
wild type enzyme, in potassium phosphate buffer (pH 6.5, 50 mM)
0.38
-
mutant enzyme H70L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.38
-
pH and temperature not specified in the publication, mutant H70L
0.4
-
mutant enzyme H281A
0.44
-
recombinant, his-tagged enzyme at pH 6.0 and 35°C
0.45
-
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C
0.49
-
mutant enzyme H281Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.49
-
pH and temperature not specified in the publication, mutant H281Y
0.51
-
mutant enzyme P24A, in potassium phosphate buffer (pH 6.5, 50 mM)
0.54
-
mutant enzyme H281F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.54
-
wild type enzyme, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
0.54
-
pH and temperature not specified in the publication, mutant H281F
0.58
-
mutant enzyme L476Q
0.58
-
mutant enzyme L476Q, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
0.59
-
mutant enzyme L476G, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
0.6
-
mutant enzyme S181T/L476P, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
0.69
-
wild type enzyme at pH 6.0 and 35°C
0.8
-
mutant enzyme S26M, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.8
-
pH 6.0, 30°C, wild-type enzyme
0.8
-
pH and temperature not specified in the publication, mutant S26M
0.85
-
mutant enzyme H70F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.85
-
mutant enzyme S26T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.85
-
pH and temperature not specified in the publication, mutant H70F
0.89
-
mutant enzyme H70T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.9
-
mutant enzyme H281T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
0.94
-
mutant enzyme H70S, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1
-
mutant enzyme L476H, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.02
-
mutant enzyme L476T, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.03
-
mutant enzyme L476P, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.06
-
mutant enzyme L476Q/S525G, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.08
-
mutant enzyme L476M, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.1
-
mutant enzyme A460I/F464I
1.1
-
pH 6.0, 30°C, mutant enzyme A460I/F464I
1.14
-
mutant enzyme L476S, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.17
-
mutant enzyme L476A, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.2
-
mutant enzyme H281A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.2
-
mutant enzyme S26L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.2
-
pH and temperature not specified in the publication, mutant H281A
1.2
-
pH and temperature not specified in the publication, mutant S26L
1.23
-
mutant enzyme S181T, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.26
-
mutant enzyme L476C, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.4
-
mutant enzyme L461G, in potassium phosphate buffer (pH 6.5, 50 mM)
1.46
-
mutant enzyme L476K, in 50 mM potassium phosphate buffer pH 7.0, 0.5 mM thiamine diphosphate, 2.5 mM MgSO4, at 30°C
1.5
-
mutant enzyme H70A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
1.5
-
pH and temperature not specified in the publication, mutant H70A
1.54
-
mutant enzyme A460G, in potassium phosphate buffer (pH 6.5, 50 mM)
1.68
-
pH 7.0, mutant enzyme H281A
1.92
-
pH 7.0, mutant enzyme H70A
2
3
wild type enzyme, at pH 6.0 and 30°C
2.3
-
mutant enzyme F464I
2.3
-
pH 6.0, 30°C, mutant enzyme F464I
2.9
-
mutant enzyme H281N in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
7.8
-
mutant enzyme S26A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C
7.8
-
pH and temperature not specified in the publication, mutant S26A
8.62
-
pH 7.0, mutant enzyme S26A
15
-
mutant enzyme I466A, at pH 6.0 and 30°C
7.5
-
in 50 mM potassium phosphate buffer, pH 6.8 in the presence of 2.5 mM MgSO4 and 0.1 mM thiamine diphosphate
0.024
-
mutant D29E, pH 6.5, 30°C
0.14
-
mutant E468D, pH 6.5, 30°C
0.27
-
mutant H115K, pH 6.5, 30°C
1.65
-
wild-type, pH 6.5, 30°C
1.71
-
mutant Q383T, pH 6.5, 30°C
2.72
-
mutant E52D, pH 6.5, 30°C
7.3
-
at pH 6.5, temperature not specified in the publication
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1997
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L-Aspartate-beta-decarboxylase. Structure, catalytic activities, and allosteric regulation
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Differential reactivity in the processing of [p-(halomethyl)benzoyl]formates by benzoylformate decarboxylase, a thiamin pyrophosphate dependent enzyme
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Kinetics and mechanism of benzoylformate decarboxylase using 13C and solvent deuterium isotope effects on benzoylformate and benzoylformate analogues
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Initial reactions involved in the dissimilation of mandelate by Rhodotorula graminis
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Negative Co-operativity in the benzoylformate decarboxylase of Acinetobacter calcoaceticus N.C.I.B. 8250
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Benzoylformate decarboxylase (Pseudomonas putida)
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307-322
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Acyloin formation by benzoylformate decarboxylase from Pseudomonas putida
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1699-1704
Purification and crystallization of benzoylformate decarboxylase
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251
907-915
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L-alpha-Hydroxy acid oxidases of hog renal cortex
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Structural and kinetic analysis of catalysis by a thiamin diphosphate-dependent enzyme, benzoylformate decarboxylase
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Branched-chain keto acid decarboxylase from Lactococcus lactis (KdcA), a valuable thiamine diphosphate-dependent enzyme for asymmetric C-C bond formation
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349
1425-1435
Identification of novel benzoylformate decarboxylases by growth selection
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46
10739-10744
Isolation and characterization of a benzoylformate decarboxylase and a NAD+/NADP+-dependent benzaldehyde dehydrogenase involved in D-phenylglycine metabolism in Pseudomonas stutzeri ST-201
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Biochim. Biophys. Acta
1770
1585-1592
Protein-enhanced decarboxylation of the covalent intermediate in benzoylformate decarboxylase--Desolvation or acid catalysis?
2006
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34
337-344
Factors mediating activity, selectivity, and substrate specificity for the thiamin diphosphate-dependent enzymes benzaldehyde lyase and benzoylformate decarboxylase
2006
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An activity, stability and selectivity comparison of propioin synthesis by thiamine diphosphate-dependent enzymes in a solid/gas bioreactor
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Chembiochem
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Asymmetric synthesis of aliphatic 2-hydroxy ketones by enzymatic carboligation of aldehydes
2007
Dominguez de Maria, P.; Pohl, M.; Gocke, D.; Groeger, H.; Trauthwein, H.; Stillger, T.; Walter, L.; Mueller, M.
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2007
2940-2944
Accelerating unimolecular decarboxylation by preassociated acid catalysis in thiamin-derived intermediates: implicating Bronsted acids as carbanion traps in enzymes
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