Ligand 4-nitrophenyl

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Basic Ligand Information

Molecular Structure
Picture of 4-nitrophenyl (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C6H5NO2
4-nitrophenyl
Synonyms:
nitrobenzene


Show all pahtways known for Show all BRENDA pathways known for 4-nitrophenyl

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
nitrobenzene + 2 NADPH + 2 H+ = N-hydroxyaniline + 2 NADP+ + H2O
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Substrate in Enzyme-catalyzed Reactions (10 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
nitrobenzene + NADH + O2 = catechol + nitrite + NAD+
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show the reaction diagram
nitrobenzene + NADH + H+ + O2 = 4-nitrophenol + NAD+ + H2O
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show the reaction diagram
nitrobenzene + NADH + O2 = nitrophenol + NAD+ + H2O
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show the reaction diagram
nitrobenzene + NADPH + H+ = ? + NADP+
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show the reaction diagram
4-nitrophenyl + H2O = 4-nitrophenol + beta-D-xylopyranose
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show the reaction diagram
NADH + H+ + nitrobenzene = NAD+ + ?
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Product in Enzyme-catalyzed Reactions (17 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
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show the reaction diagram
4-nitrophenyl laurate + H2O = 4-nitrophenyl + laurate
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show the reaction diagram
4-nitrophenyl phosphate + H2O = 4-nitrophenyl + phosphate
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show the reaction diagram
4-nitrophenyl-alpha-D-mannopyranoside + H2O = 4-nitrophenyl + alpha-D-mannose
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show the reaction diagram
4-nitrophenyl alpha-D-glucopyranoside + H2O = 4-nitrophenyl + D-glucose
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show the reaction diagram
4-nitrophenyl-alpha-L-arabinopyranoside + H2O = 4-nitrophenyl + L-arabinopyranose
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show the reaction diagram
4-nitrophenyl beta-D-glucoside + H2O = 4-nitrophenyl + D-glucose
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show the reaction diagram
benzyloxycarbonyl-Arg-p-nitrobenzyl ester + H2O = benzyloxycarbonyl-Arg + 4-nitrophenyl
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show the reaction diagram
benzyloxycarbonyl-Ala-4-nitrophenyl ester + H2O = benzyloxycarbonyl-Ala + 4-nitrophenyl
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show the reaction diagram
N-carbobenzoxy-Lys-p-nitrophenyl ester + H2O = N-carbobenzoxy-Lys + 4-nitrophenyl
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Inhibitor in Enzyme-catalyzed Reactions (1 result)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (10 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
mutant A405G, pH 6.8, 30°C
0.23
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mutant I204A, pH 6.8, 30°C
0.19
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mutant I204T, pH 6.8, 30°C
0.22
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mutant I204V, pH 6.8, 30°C
0.25
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pH 6.8, 30°C
0.3
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wild-type, pH 6.8, 30°C
0.27
-
mutant T201A, pH 7.5, 4°C
0.034
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wild-type, pH 7.5, 4°C
0.013
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pH 8.0, 37°C
0.18
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pH 7.0, 25°C
14
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KM Value (16 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
mutant A405G, pH 6.8, 30°C
0.0062
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mutant F293H, pH 7.3, 30°C
0.0239
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mutant F293Q, pH 7.3, 30°C
0.0607
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mutant I204A, pH 6.8, 30°C
0.047
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mutant I204T, pH 6.8, 30°C
0.0099
-
mutant I204V, pH 6.8, 30°C
0.0048
-
mutant I350T, pH 7.3, 30°C
0.159
-
mutant N258V, pH 7.3, 30°C
1.78
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pH 6.8, 30°C
0.0045
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wild-type, pH 6.8, 30°C
0.0047
-
wild-type, pH 7.3, 30°C
0.0077
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25°C, pH not specified in the publication
0.0023
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imobilized enzyme, 25°C, pH not specified in the publication
0.002
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pH 8.0, 37°C
0.545
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temperature not specified in the publication, pH 8.0
0.005
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pH 7.0, 25°C
1.5
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Ki Value (1 result)

COMMENTARY
EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
LITERATURE
-
0.205
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References & Links

Links to other databases for 4-nitrophenyl

ChEBI
PubChem
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