Ligand amylopectin

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Basic Ligand Information

Molecular Structure
Picture of amylopectin (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C60H99O48R3
amylopectin
Synonyms:
amylose-extender amylopectin
Pathway Source
Pathways
MetaCyc
starch biosynthesis, starch degradation II

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (18 results)

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EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
amylopectin + ascorbic acid + O2 = malto-aldonic acids + dehydroascorbate
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show the reaction diagram
amylopectin = amylopectin containing alpha-1,6-glucosidic linkages
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show the reaction diagram
amylopectin + H2O = ?
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show the reaction diagram
amylopectin + H2O = alpha-D-glucose
-
show the reaction diagram
amylopectin + H2O = beta-D-glucose
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show the reaction diagram
amylopectin + H2O = amylopectin + D-glucose
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In Vivo Product in Enzyme-catalyzed Reactions (1 result)

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EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
amylopectin + H2O = amylopectin + D-glucose
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Substrate in Enzyme-catalyzed Reactions (95 results)

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EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
amylopectin + ascorbic acid + O2 = malto-aldonic acids + dehydroascorbate
-
show the reaction diagram
ADP-glucose + amylopectin = ADP + ?
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show the reaction diagram
sucrose + amylopectin = ?
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show the reaction diagram
amylopectin + H2O = ?
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show the reaction diagram
amylopectin + H2O = ?
-
show the reaction diagram
amylopectin + H2O = ?
-
show the reaction diagram
amylopectin + H2O = maltotetraose + ?
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show the reaction diagram
amylopectin + H2O = ?
-
show the reaction diagram
amylopectin + H2O = ?
-
show the reaction diagram
amylopectin + H2O = ?
-
show the reaction diagram
amylopectin + H2O = maltose + ?
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Product in Enzyme-catalyzed Reactions (5 results)

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EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
amylopectin + H2O = amylopectin + beta-D-glucose
-
show the reaction diagram
amylopectin + H2O = amylopectin + D-glucose
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Activator in Enzyme-catalyzed Reactions (2 results)

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COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (47 results)

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COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
pH 8.0, 30°C
235
-
recombinant His6-tagged enzyme, pH 5.0, 20°C
3.4
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calculated as low molecular weight products
171
-
5°C, pH 8.3
0.042
-
45°C, pH 5.0
137
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isozyme HaAmy1, pH 7.0, 37°C
0.29
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isozyme HaAmy2, pH 7.0, 37°C
0.13
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pH 5.0, 37°C, recombinant enzyme
pH 6.5, 90°C
4180
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pH 7.0, 50°C
2274.8
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15°C, pH 8.3
0.15
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recombinant His-tagged enzyme, pH 5.0, 45°C
137
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pH 6.5, 50°C
19.4
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monomeric enzyme form, at pH 7.0 and 80°C
0.17
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in sodium-acetate buffer (50 mM, pH 6.0), at 60°C
15.38
-
mutant enzyme D109A, at pH 7.0 and 80°C
0.1
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dimeric enzyme form, at pH 7.0 and 80°C
0.04
-
at pH 5.5 and 90°C
4.01
-
mutant enzyme D109E, at pH 7.0 and 80°C
0.005
-
pH and temperature not specified in the publication
0.8
-
mutant M440G, 37°C, pH not specified in the publication
3.9
-
wild-type, 37°C, pH not specified in the publication
15.6
-
37°C, recombinant mutant Y164Q
453
-
-
550
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37°C, recombinant mutant T47M/Y164E/T328N
325
-
37°C, recombinant mutant Y164E
1129
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pH 5.4, 37°C, recombinant wild-type enzyme
1280
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pH 5.4, 37°C, recombinant mutant T342V
pH 5.4, 37°C, recombinant mutant T342S
3.5
-
pH 5.4, 37°C, recombinant mutant T342A
0.75
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37°C, recombinant mutant Y164F
988
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37°C, recombinant mutant Y164H
92
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37°C, recombinant wild-type enzyme
2739
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pH 4.5, 37°C
24.2
-
-
25.48
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pH 5.0, 55°C
34.1
-
-
683
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pH 5.5, 75°C, calculated as microMol maltose released from the substrate, in absence of DMSO
0.923
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pH 5.5, 75°C, calculated as microMol maltose released from the substrate, in presence of DMSO
pH 6.5, 75°C, calculated as microMol maltose released from the substrate, in absence of DMSO
0.48
-
pH 3.5, 95°C
1.69
-
37°C, pH 6.0
2.18
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KM Value (54 results)

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COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
stromal phosphorylase, synthesis
0.017
-
stromal phosphorylase, phosphorolysis
0.028
-
pH 7.5, 37°C, synthesis reaction, cytosolic isozyme
0.03
-
pH 7.5, 30°C, degradation reaction, cytosolic isozyme
0.03
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pH 5.5, 37°C, synthesis reaction
0.0046
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low-mobility isoform
10
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high-mobility isoform
2
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pH 6.4, 30°C, type IV amylopectin
0.0056
-
-
0.75
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UDP-glucose utilizing enzyme in presence of 0.5 M citrate
0.52
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Km value is 1.9 ml/ml at 5°C, pH 8.3 and 2.5 mg/ml at 15°C, pH 8.3
-999
-
micromol of D-glucosidic bond per liter
18.2
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Km-value: 8.95 mg/ml, 45°C, pH 5.0
-999
-
Km-value: 10.3 mg/ml, pH 7.0, 50°C
-999
-
0.0012 mg/ml, intestine alpha-amylase
-999
-
0.002 mg/ml, muscle alpha-amylase
-999
-
calculated as low molecular weight products
3.5
-
pH 9, 50°C, Km-value shown as glucose equivalents
49
-
Km-value: 8.1 mg/ml, pH 6.5, 50°C
-999
-
Km value of wild-type 6.9 mg/ml, mutant M440G 4.4 mg/ml, respectively, 37°C, pH not specified in the publication
-999
-
KM value 0.056 mg/ml, pH 7.0, 25°C
-999
-
pH 5.4, 37°C, recombinant mutant T342V
1.84
-
37°C, recombinant mutant Y164H
2.63
-
37°C, recombinant mutant Y164Q
1.22
-
37°C, recombinant mutant Y164F
0.92
-
pH 5.4, 37°C, recombinant mutant T342A
0.26
-
37°C, recombinant wild-type enzyme
0.73
-
pH 5.4, 37°C, recombinant mutant T342S
0.39
-
pH 5.4, 37°C, recombinant wild-type enzyme
1.94
-
37°C, recombinant mutant Y164E
1.24
-
37°C, recombinant mutant T47M/Y164E/T328N
0.47
-
0.11 mg/ml
-999
-
pH 4.5, 37°C
0.0013
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pH 5.0, 37°C, isozyme GA-I
17.1
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enzyme form GIII
0.3
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pH 5.0, 37°C, isozyme GA-II
4.2
-
pH 5.0, 55°C
2
-
enzyme form GI
6.1
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glucoamylase M2
1.65
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glucoamylase G5
0.16
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glucoamylase G4
0.17
-
glucoamylase G2 or G3
0.0002
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glucoamylase G1
0.0003
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enzyme form GII
7.4
-
Km-values: 31.1 mg/ml at pH 5.5, 75°C, in absence of DMSO. 88.0 mg/ml at pH 5.5, 75°C, in presence of DMSO. 92.3 mg/ml at pH 6.5, 75°C, in absence of DMSO. 345 mg/ml at pH 5.5, 75°C, in presence of DMSO
-999
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Km-value: 3.95 mg/ml, pH 3.5, 95°C
-999
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Km-value: 3.6 mg/ml, pH 6.5, 55°C
-999
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homo-oligomer
hetero-oligomer
pH 5.0, 35°C, barley amylopectin or potato amylopectin
0.03
-

References & Links

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Links to other databases for amylopectin

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ChEBI
PubChem
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