print visible entries

print all entries

Ligand GlcNAcMan5GlcNAc2

Basic Ligand Information

Molecular Structure

close all tables

open all tables

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

top print hide

Show Natural Substrate (2 entries)

EC NUMBER

LITERATURE

REACTION DIAGRAM

PROVEN IN VIVO REACTION

ENZYME 3D STRUCTURE

3.2.1.114

715284

GlcNAcMan5GlcNAc2 + H2O = ? + alpha-D-mannose

-

3.2.1.114

646641, 646640, 646602

Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O = Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose

-

3.2.1.114

2 entries

Substrate in Enzyme-catalyzed Reactions (4 results)

top print hide

Show Substrate (4 entries)

EC NUMBER

LITERATURE

REACTION DIAGRAM

REACTION

ENZYME 3D STRUCTURE

3.2.1.114

664599

GlcNAcMan5GlcNAc2 + H2O = ?

-

3.2.1.114

715284

GlcNAcMan5GlcNAc2 + H2O = ? + alpha-D-mannose

-

3.2.1.114

646637, 646641, 646640, 646617, 646602

Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O = Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose

-

3.2.1.114

681767, 682539

GlcNAc(Man)5GlcNAc2 + H2O = GlcNAc(Man)3GlcNAc2 + D-mannose

-

3.2.1.114

4 entries

Enzyme Kinetic Parameters

References & Links

Literature References (9)

top print hide

Show Reference (9 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

646602

14636047

Comparison of kifunensine and 1-deoxymannojirimycin binding to class I and II alpha-mannosidases demonstrates different saccharide distortions in inverting and retaining catalytic mechanisms

2003

Shah, N.; Kuntz, D.A.; Rose, D.R.

Biochemistry

42

13812-13816

646617

6445359

Control of glycoprotein synthesis. Processing of asparagine-linked oligosaccharides by one or more rat liver Golgi alpha-D-mannosidases dependent on the prior action of UDP-N-acetylglucosamine: alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I

1980

Harpaz, N.; Schachter, H.

J. Biol. Chem.

255

4894-4902

646637

9210421

Purification and properties of alpha-mannosidase II from Golgi-like membranes of baculovirus-infected Spodoptera frugiperda (IPLB-SF-21AE) cells

1997

Ren, J.; Castellino, F.J.; Bretthauer, R.K.

Biochem. J.

324

951-956

646640

10472797

alpha-Mannosidases involved in N-glycan processing show cell specificity and distinct subcompartmentalization within the Golgi apparatus of cells in the testis and epididymis

1999

Igdoura, S.A.; Herscovics, A.; Lal, A.; Moremen, K.W.; Morales, C.R.; Hermo, L.

Eur. J. Cell Biol.

78

441-452

646641

11231279

Overexpression of the Golgi-localized enzyme alpha-mannosidase IIx in Chinese hamster ovary cells results in the conversion of hexamannosyl-N-acetylchitobiose to tetramannosyl-N-acetylchitobiose in the N-glycan-processing pathway

2001

Oh-Eda, M.; Nakagawa, H.; Akama, T.O.; Lowitz, K.; Misago, M.; Moremen, K.W.; Fukuda, M.N.

Eur. J. Biochem.

268

1280-1288

664599

15368573

Inhibition of Golgi mannosidase II with mannostatin A analogues: synthesis, biological evaluation, and structure-activity relationship studies

2004

Li, B.; Kawatkar, S.P.; George, S.; Strachan, H.; Woods, R.J.; Siriwardena, A.; Moremen, K.W.; Boons, G.J.

Chembiochem

5

1220-1227

681767

17113875

N-Glycan structure analysis using lectins and an alpha-mannosidase activity assay

2006

Akama, T.O.; Fukuda, M.N.

Methods Enzymol.

416

304-314

682539

16754854

Essential and mutually compensatory roles of {alpha}-mannosidase II and {alpha}-mannosidase IIx in N-glycan processing in vivo in mice

2006

Akama, T.O.; Nakagawa, H.; Wong, N.K.; Sutton-Smith, M.; Dell, A.; Morris, H.R.; Nakayama, J.; Nishimura, S.; Pai, A.; Moremen, K.W.; Marth, J.D.; Fukuda, M.N.

Proc. Natl. Acad. Sci. USA

103

8983-8988

715284

20504027

Molecular mechanism of the glycosylation step catalyzed by Golgi alpha-mannosidase II: a QM/MM metadynamics investigation

2010

Petersen, L.; Ardevol, A.; Rovira, C.; Reilly, P.J.

J. Am. Chem. Soc.

132

8291-8300

Links to other databases for GlcNAcMan5GlcNAc2

top print hide

Show Links (1 entry)

ChEBI

PubChem

-