Ligand 5-hydroxymethyluracil

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Basic Ligand Information

Molecular Structure
Picture of 5-hydroxymethyluracil (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C5H6N2O3
5-hydroxymethyluracil
JDBGXEHEIRGOBU-UHFFFAOYSA-N
Synonyms:
5-(hydroxymethyl)pyrimidine-2,4(1H,3H)-dione, 5-Hydroxymethyl-uracil

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
5-hydroxymethyluracil + 2-oxoglutarate + O2 = 5-formyluracil + succinate + CO2
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (6 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2
show the reaction diagram
-
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O = 5-hydroxymethyluracil + double-stranded DNA with abasic site
show the reaction diagram
-

Substrate in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
5-hydroxymethyluracil + 2-oxoglutarate + O2 = 5-formyluracil + succinate + CO2
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (6 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2
show the reaction diagram
-
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O = 5-hydroxymethyluracil + double-stranded DNA with abasic site
show the reaction diagram
-

Inhibitor in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
competitive inhibitor against thymine, noncompetitive against oxygen
-
35% inactivation at 2 mM
-
; inhibition of isozyme hCA I
-

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (1 result)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.42
-
pH 7.5, 30°C, wild-type enzyme

KM Value (3 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

Ki Value (1 result)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.04951
-
pH 7.4, temperature not specified in the publication

References & Links

Links to other databases for 5-hydroxymethyluracil

ChEBI
PubChem
ChEBI
PubChem