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Ligand alpha,alpha-trehalose

Basic Ligand Information

Molecular Structure

Pathway Source

Pathways

BRENDA

metabolism of disaccharids

KEGG

ABC transporters, Biosynthesis of secondary metabolites, Phosphotransferase system (PTS), Starch and sucrose metabolism

MetaCyc

glycogen biosynthesis III (from alpha-maltose 1-phosphate), mycobacterial sulfolipid biosynthesis, mycolate biosynthesis, mycolyl-arabinogalactan-peptidoglycan complex biosynthesis, polyacyltrehalose biosynthesis, trehalose biosynthesis I, trehalose biosynthesis II, trehalose biosynthesis III, trehalose biosynthesis IV, trehalose biosynthesis V, trehalose biosynthesis VI, trehalose biosynthesis VII, trehalose degradation II (cytosolic), trehalose degradation IV, trehalose degradation V, trehalose degradation VI (periplasmic) more

Show all BRENDA pathways known for alpha,alpha-trehalose

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (13 results)

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Show Natural Substrate (13 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.1.122

alpha,alpha-trehalose 6-mycolate + alpha,alpha-trehalose = alpha,alpha-trehalose + alpha,alpha-trehalose 6-mycolate

486242

-

2.3.1.279

a long-chain-fatty acyl-CoA + alpha,alpha-trehalose = a 2-(long-chain-fatty acyl)-trehalose + CoA

750956, 751021, 751039

-

2.4.1.231

alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D-glucose 1-phosphate

644902, 644899, 644897, 644898, 644900, 644905, 644903, 644906, 644901, 644904, 644907

-

2.4.1.64

alpha,alpha-trehalose + phosphate = D-glucose + beta-D-glucose 1-phosphate

736104, 489323

-

2.7.1.201

[protein]-Npi-phospho-L-histidine + alpha,alpha-trehalose[side 1] = [protein]-L-histidine + alpha,alpha-trehalose 6-phosphate[side 2]

736332, 737143, 736763, 736338

-

2.8.2.37

3'-phosphoadenylyl sulfate + alpha,alpha-trehalose = adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose

729533, 726029, 723932

-

3.2.1.21

trehalose + H2O = beta-D-glucose + ?

326295, 326283

-

3.2.1.28

alpha,alpha-trehalose + H2O = 2 D-glucose

666654, 665191, 710386, 664188, 708733, 655599, 665006, 665566, 664664, 665197, 708486

-

3.2.1.28

alpha,alpha-trehalose + H2O = alpha-D-glucopyranose + D-glucose

657031, 654532

-

3.2.1.28

alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose

707785

-

3.2.1.28

alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose

730763, 729103, 730096, 729539, 730924, 750527, 731011, 729110, 750882, 729803, 729830, 730653, 749959, 751266, 754350

-

3.2.1.28

trehalose + H2O = 2 D-glucose

709841, 710219, 704913, 709400

-

3.2.1.28

trehalose + H2O = D-glucose

136176, 136178, 136173, 136146, 136169, 136167

-

3.2.1.28

6 entries

In Vivo Product in Enzyme-catalyzed Reactions (10 results)

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Show Natural Product (10 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.1.122

2 alpha,alpha-trehalose 6-mycolate = alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate

486242, 0

-

2.3.1.122

alpha,alpha-trehalose 6-mycolate + alpha,alpha-trehalose = alpha,alpha-trehalose + alpha,alpha-trehalose 6-mycolate

486242

-

2.3.1.122

alpha,alpha-trehalose 6-mycolate = alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate

0

-

2.3.1.122

3 entries

2.4.1.245

ADP-alpha-D-glucose + D-glucose = ADP + alpha,alpha-trehalose

0

-

2.4.1.64

D-glucose + beta-D-glucose 1-phosphate = alpha,alpha-trehalose + phosphate

0

-

3.1.3.12

alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate

0

-

3.1.3.12

trehalose 6-phosphate + H2O = trehalose + phosphate

0, 488614, 649392, 649561, 652396, 651667, 651481, 651386, 653540

-

3.1.3.12

trehalose-6-phosphate + H2O = trehalose + phosphate

0, 649561, 653157

-

3.1.3.12

3 entries

3.2.1.141

4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O = (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose

0

-

5.4.99.16

maltose = alpha,alpha-trehalose

0

-

Substrate in Enzyme-catalyzed Reactions (53 results)

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Show Substrate (53 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.3.10

D-trehalose + O2 = ?

686372

-

1.1.3.10

trehalose + O2 = ?

655049

-

1.1.5.9

alpha,alpha-trehalose + 2,6-dichlorophenolindophenol = ? + reduced 2,6-dichlorophenolindophenol

740853

-

1.1.5.9

trehalose + 2,6-dichlorophenolindophenol = ? + reduced 2,6-dichlorophenolindophenol

740778

-

1.1.99.13

trehalose + 2,6-dichlorophenolindophenol = ?

739878

-

1.1.99.13

trehalose + 2,6-dichlorophenolindophenol = ? + reduced 2,6-dichlorophenolindophenol

741470

-

1.1.99.13

trehalose + acceptor = ?

389892, 389893, 389900, 389895, 389896, 655729

-

1.1.99.29

alpha,alpha-trehalose + 1,4-benzoquinone = 3,3'-didehydro-alpha,alpha-trehalose + hydroquinone

644548

-

1.1.99.29

alpha,alpha-trehalose + acceptor = 3,3'-didehydro-alpha,alpha-trehalose + reduced acceptor

695788

-

1.1.99.29

D-trehalose + acceptor = ?

686372

-

1.1.99.29

trehalose + 1,4-benzoquinone = ?

644547, 644544

-

1.1.99.29

trehalose + ferricenium ion = ?

644547

-

2.3.1.122

alpha,alpha-trehalose 6-mycolate + alpha,alpha-trehalose = alpha,alpha-trehalose + alpha,alpha-trehalose 6-mycolate

486242

-

2.3.1.122

alpha,alpha-trehalose 6-palmitate + trehalose = alpha,alpha-trehalose + alpha,alpha-trehalose 6-palmitate

486242

-

1.1.3.10

2 entries

1.1.5.9

2 entries

1.1.99.13

3 entries

1.1.99.29

5 entries

2.3.1.122

2 entries

2.3.1.279

a long-chain-fatty acyl-CoA + alpha,alpha-trehalose = a 2-(long-chain-fatty acyl)-trehalose + CoA

750956, 751021, 751039

-

2.4.1.230

alpha,alpha-trehalose + phosphate = D-glucose + beta-D-glucose 1-phosphate

736088

-

2.4.1.231

alpha,alpha-trehalose + arsenate = alpha-D-glucose + alpha-D-glucose 1-arsenate

674984

-

2.4.1.231

alpha,alpha-trehalose + arsenate = alpha-D-glucose 1-arsenate + alpha-D-glucose

644898, 644897, 674984

-

2.4.1.231

alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D-glucose 1-phosphate

644907, 644902, 720962, 644899, 691645, 692323, 644897, 644898, 644900, 644905, 644903, 674984, 644906, 644901, 644904, 757619, 757361

-

2.4.1.245

alpha,alpha-1,1-trehalose + ADP = ADP-glucose + D-glucose

662295

-

2.4.1.245

alpha,alpha-trehalose + ADP = ADP-alpha-D-glucose + D-glucose

723675, 747674

-

2.4.1.245

alpha,alpha-trehalose + CDP = CDP-alpha-D-glucose + D-glucose

747674

-

2.4.1.245

alpha,alpha-trehalose + GDP = GDP-alpha-D-glucose + D-glucose

723675, 747674

-

2.4.1.245

alpha,alpha-trehalose + UDP = UDP-alpha-D-glucose + D-glucose

723675, 747674

-

2.4.1.64

alpha,alpha-trehalose + phosphate = alpha-D-glucose + beta-D-glucose 1-phosphate

693804

-

2.4.1.64

alpha,alpha-trehalose + phosphate = beta-D-glucose 1-phosphate + D-glucose

721191, 721346

-

2.4.1.64

alpha,alpha-trehalose + phosphate = D-glucose + beta-D-glucose 1-phosphate

489320, 489321, 489322, 489323, 134592, 489326, 672930, 721896, 489328, 489325, 736104

-

2.4.1.231

3 entries

2.4.1.245

5 entries

2.4.1.64

3 entries

2.7.1.201

[protein]-Npi-phospho-L-histidine + alpha,alpha-trehalose[side 1] = [protein]-L-histidine + alpha,alpha-trehalose 6-phosphate[side 2]

736332, 737143, 736763, 736338

-

2.8.2.37

3'-phosphoadenylyl sulfate + alpha,alpha-trehalose = adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose

729533, 723932, 726029

-

3.1.1.6

alpha,alpha-trehalose + vinyl acetate = ? + vinyl alcohol

715029

-

3.2.1.10

trehalose + H2O = 2 alpha-D-glucose

690556

-

3.2.1.10

trehalose + H2O = ?

655923, 654304

-

3.2.1.10

2 entries

3.2.1.106

trehalose = ?

699548

-

3.2.1.20

alpha,alpha-1,1-trehalose + H2O = ?

692850

-

3.2.1.20

trehalose + H2O = ?

655752

-

3.2.1.20

trehalose + H2O = alpha-D-glucose

393346, 393333, 393349, 654259, 692850, 393334, 393344

-

3.2.1.20

3 entries

3.2.1.207

trehalose + H2O = ?

713777

-

3.2.1.21

trehalose + H2O = beta-D-glucose + ?

326295, 326283

-

3.2.1.22

trehalose + phenyl alpha-D-galactoside = ?

171187

-

3.2.1.26

trehalose + H2O = ?

136127, 136099

-

3.2.1.28

alpha,alpha-trehalose + H2O = 2 D-glucopyranose

701068

-

3.2.1.28

alpha,alpha-trehalose + H2O = 2 D-glucose

666654, 715128, 665006, 708486, 665197, 715680, 655599, 664664, 665566, 665191, 710386, 664188, 741949, 655304, 664996, 708733, 715178, 716128, 679765, 741947

-

3.2.1.28

alpha,alpha-trehalose + H2O = alpha-D-glucopyranose + D-glucose

655983, 656417, 655271, 654532, 657031, 655304, 656876, 655006

-

3.2.1.28

alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose

707785

-

3.2.1.28

alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose

730763, 729103, 730096, 750739, 729539, 730924, 750527, 731011, 739003, 729110, 750129, 750882, 729803, 729830, 750191, 751266, 754350, 750693, 750787, 751313, 749959, 730653

-

3.2.1.28

alpha-alpha-trehalose + H2O = D-glucose

656848

-

3.2.1.28

trehalose + H2O = 2 D-glucose

709841, 710219, 704913, 709400

-

3.2.1.28

trehalose + H2O = D-glucose

136153, 136173, 136178, 136172, 136157, 136142, 136168, 136141, 136150, 136158, 136162, 136165, 136174, 136175, 677711, 678895, 679927, 136169, 136171, 681232, 136143, 136145, 136147, 136148, 136149, 136161, 679865, 136152, 136166, 681784, 136159, 681792, 680519, 136167, 136163, 136154, 682056, 136146, 136155, 136177, 136180, 136170, 136176, 136179, 682142, 678800, 677555, 136160, 136144, 136151, 136156, 136164, 679712, 682673, 680542

-

3.2.1.3

alpha-alpha-trehalose + H2O = alpha-D-glucose

136228

-

3.2.1.3

trehalose + H2O = D-glucose

136199

-

3.2.1.28

8 entries

3.2.1.3

2 entries

3.2.1.74

trehalose + H2O = ?

136844

-

3.2.1.94

dextran + trehalose = 6-alpha-isomaltosyltrehalose + H2O

137024

-

5.4.99.16

alpha,alpha-trehalose = maltose

747681, 727433, 759128, 760081, 759622, 680282, 759527, 759389

-

Product in Enzyme-catalyzed Reactions (47 results)

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Show Product (47 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.1.122

alpha,alpha'-trehalose 6-monomycolate + alpha,alpha'-trehalose 6-monomycolate = alpha,alpha'-trehalose 6,6'-dimycolate + alpha,alpha'-trehalose

0

-

2.3.1.122

2 alpha,alpha-trehalose 6-monomycolate = alpha,alpha-trehalose 6,6'-dimycolate + alpha,alpha-trehalose

0

-

2.3.1.122

2 alpha,alpha-trehalose 6-mycolate = alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate

486242, 0

-

2.3.1.122

alpha,alpha-trehalose 6-mycolate + alpha,alpha-trehalose = alpha,alpha-trehalose + alpha,alpha-trehalose 6-mycolate

486242

-

2.3.1.122

alpha,alpha-trehalose 6-mycolate = alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate

0

-

2.3.1.122

alpha,alpha-trehalose 6-palmitate + trehalose = alpha,alpha-trehalose + alpha,alpha-trehalose 6-palmitate

486242

-

2.3.1.122

alpha,alpha-trehalose monomycolate + D-glucose = D-glucose monomycolate + alpha,alpha-trehalose

0

-

2.3.1.122

7 entries

2.4.1.15

alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate

0

-

2.4.1.231

alpha-D-glucose + alpha-D-glucose 1-phosphate = alpha,alpha-trehalose + phosphate

0

-

2.4.1.245

ADP-alpha-D-glucose + D-glucose = ADP + alpha,alpha-1,1-trehalose

0

-

2.4.1.245

GDP-glucose + D-glucose = alpha,alpha-1,1-trehalose + GDP

0

-

2.4.1.245

UDP-glucose + D-glucose = alpha,alpha-1,1-trehalose + UDP

0

-

2.4.1.245

ADP-alpha-D-glucose + D-glucose = ADP + alpha,alpha-trehalose

0

-

2.4.1.245

ADP-alpha-D-glucose + D-glucose = alpha,alpha-trehalose + ADP

0

-

2.4.1.245

CDP-alpha-D-glucose + D-glucose = alpha,alpha-trehalose + CDP

0

-

2.4.1.245

GDP-alpha-D-glucose + D-glucose = alpha,alpha-trehalose + GDP

0

-

2.4.1.245

GDP-glucose + D-glucose = alpha,alpha-trehalose + GDP

0

-

2.4.1.245

UDP-alpha-D-glucose + D-glucose = alpha,alpha-trehalose + UDP

0

-

2.4.1.245

UDP-glucose + D-glucose = alpha,alpha-trehalose + UDP

0

-

2.4.1.245

UDP-alpha-D-glucose + D-galactose = alpha-D-Glc-(1->1)-alpha-D-Glc + UDP

0

-

2.4.1.64

beta-D-glucose 1-phosphate + D-glucose = alpha,alpha-trehalose + phosphate

0

-

2.4.1.64

D-glucose + beta-D-glucose 1-phosphate = alpha,alpha-trehalose + phosphate

0

-

2.4.1.245

11 entries

2.4.1.64

2 entries

3.1.3.1

trehalose 6-phosphate + H2O = trehalose + phosphate

0

-

3.1.3.101

trehalose 6-phosphate + H2O = trehalose + phosphate

0

-

3.1.3.12

alpha,alpha-1,1-trehalose 6-phosphate = alpha,alpha-1,1-trehalose + phosphate

0

-

3.1.3.12

alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate

0

-

3.1.3.12

trehalose 6-phosphate + H2O = trehalose + phosphate

0, 488614, 649392, 649561, 653540, 652396, 651667, 651481, 651386

-

3.1.3.12

trehalose-6-phosphate + H2O = trehalose + phosphate

134589, 0, 134590, 134591, 134593, 649561, 134586, 134592, 134587, 134588, 134594, 653157

-

3.2.1.141

4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O = (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose

0

-

3.2.1.141

maltoheptaosyltrehalose + H2O = maltoheptaose + alpha,alpha'-trehalose

0

-

3.2.1.141

maltooligosyltrehalose + H2O = maltooligosaccharide + alpha,alpha'-trehalose

0

-

3.2.1.141

maltopentaosyltrehalose + H2O = maltopentaose + alpha,alpha'-trehalose

0

-

3.2.1.141

maltosyltrehalose + H2O = maltose + alpha,alpha'-trehalose

26155, 0

-

3.2.1.141

maltotetraosyltrehalose + H2O = maltotetraose + alpha,alpha'-trehalose

26155, 0

-

3.2.1.141

maltotriosyltrehalose + H2O = maltotriose + alpha,alpha'-trehalose

26155, 0

-

3.2.1.141

glucosyl trehalose + H2O = glucose + trehalose

26155

-

3.2.1.141

maltooligosyltrehalose + H2O = trehalose + ?

0

-

3.2.1.141

maltosylpentaosyltrehalose + H2O = trehalose + maltopentaose

0

-

3.2.1.141

maltosyltetraosyltrehalose + H2O = trehalose + maltotetraose

0

-

3.2.1.141

maltosyltrehalose + H2O = trehalose + maltose

0

-

3.2.1.141

maltosyltriosyltrehalose + H2O = trehalose + maltotriose

0

-

3.2.1.141

maltotriosyltrehalose + H2O = trehalose + maltotriose

0

-

3.1.3.12

4 entries

3.2.1.141

14 entries

5.4.99.15

soluble starch = trehalose

0

-

5.4.99.16

high-maltose rice syrup = alpha,alpha-trehalose

0

-

5.4.99.16

maltose = alpha,alpha-trehalose

3489, 3491, 0, 3493, 3490

-

5.4.99.16

maltose = alpha,alpha-trehalose + D-glucose + maltose

0

-

5.4.99.16

starch = alpha,alpha-trehalose

0

-

5.4.99.16

4 entries

Activator in Enzyme-catalyzed Reactions (11 results)

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Show Activating Compound (11 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.49

marginally increases the refolding yield in the presence of Arg, and it is unable to replace Arg in promoting a high refolding yield

696962

-

1.1.3.4

trehalose does not affect Vmax but instead decreases Km and as a result enzyme efficiency is increased

699078

-

2.4.1.15

activates up to 25fold

737237

-

2.4.1.19

the beta-CGTase and gamma-CGTase activities increase as the mycose concentrations increases to 2% (w/v)

758261

-

2.7.1.11

activates

675940

-

2.7.1.201

enzyme activity is induced by trehalose in the medium but not at high osmolarity

736332

-

2.7.13.3

stimulates, stimulation pattern of MtrA is similar to that of MtrB

680813

-

3.1.3.12

activates hydrolysis of glucose 6-phosphate, no activation of trehalose 6-phosphate

134587

-

3.2.1.28

the enzymatic activity increases with increasing concentrations of alpha,alpha-trehalose in the incubations up to about 50 mM

679765

-

3.2.1.3

presence of sorbitol and trehalose significantly increase the substrate affinity and catalytic efficiency of glucoamylases GAM-1 and GAM-2

730189

-

3.2.1.8

activates both isozymes and stabilizes them at higher temperatures

655381

-

Inhibitor in Enzyme-catalyzed Reactions (12 results)

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Show Inhibitors (12 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.2.1.12

might be an inhibitor, trehalose induces a conformational change in ecGAPDH in the current structure. The rotation of GAPDH also induced a conformational change in its active site. This suggests that the binding of trehalose to GAPDH induced a conformational change in its active site to prevent the binding of NAD+, although the NAD+- and trehalose-binding sites differ from one another

762577

-

2.4.1.1

weak

488230

-

2.4.1.15

non-competitive

488594

-

2.4.1.15

non-competitive to UDPglucose

488599

-

2.4.1.15

2 entries

2.4.1.64

-

736104

-

3.1.3.12

-

730793

-

3.1.3.24

-

682788

2b1q, 3D-view

3.2.1.20

inhibits both glycosyl hydrolase and transglycosylase activities decreasing Vmax by 3.9-4.8fold

682602

-

3.2.1.20

no inhibition of maltose hydrolysis, competitive inhibition of glycogen hydrolysis

393325

-

3.2.1.20

2 entries

3.2.1.26

50 mM, 23% inhibition

136100

-

3.4.22.32

bromelain in the presence of 1 M trehalose is destabilized under thermal stress, the average melting temperature decrease by 7°C, additionally the enzyme is inactivated faster at 60°C

682699

-

4.2.2.8

-

714553

-

3D Structure of Enzyme-Ligand-Complex (PDB) (136 results)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (136 entries)

EC NUMBER

ENZYME 3D STRUCTURE

1.1.1.27

1v6a, 3D-view

1.1.5.2

3a9g, 3D-view

1.1.5.2

3a9h, 3D-view

1.1.5.2

3a9g, 3D-view

1.1.5.2

3a9h, 3D-view

1.1.5.2

3a9g, 3D-view

1.1.5.2

3a9h, 3D-view

1.1.5.2

3a9g, 3D-view

1.1.5.2

3a9h, 3D-view

1.1.5.2

3a9g, 3D-view

1.1.5.2

3a9h, 3D-view

1.1.5.2

3a9g, 3D-view

1.1.5.2

3a9h, 3D-view

1.1.5.2

3a9g, 3D-view

1.1.5.2

3a9h, 3D-view

1.1.5.2

3a9g, 3D-view

1.1.5.2

3a9h, 3D-view

1.1.5.2

3a9g, 3D-view

1.1.5.2

3a9h, 3D-view

1.1.5.2

3a9g, 3D-view

1.1.5.2

3a9h, 3D-view

1.1.5.2

3a9g, 3D-view

1.1.5.2

3a9h, 3D-view

1.1.5.2

22 entries

1.14.99.3

1ni6, 3D-view

1.15.1.1

3lio, 3D-view

1.15.1.1

3lj9, 3D-view

1.15.1.1

3ljf, 3D-view

1.15.1.1

3lio, 3D-view

1.15.1.1

3lj9, 3D-view

1.15.1.1

3ljf, 3D-view

1.15.1.1

3lio, 3D-view

1.15.1.1

3lj9, 3D-view

1.15.1.1

3ljf, 3D-view

1.15.1.1

3lio, 3D-view

1.15.1.1

3lj9, 3D-view

1.15.1.1

3ljf, 3D-view

1.15.1.1

3lio, 3D-view

1.15.1.1

3lj9, 3D-view

1.15.1.1

3ljf, 3D-view

1.15.1.1

3lio, 3D-view

1.15.1.1

3lj9, 3D-view

1.15.1.1

3ljf, 3D-view

1.15.1.1

3lio, 3D-view

1.15.1.1

3lj9, 3D-view

1.15.1.1

3ljf, 3D-view

1.15.1.1

3lio, 3D-view

1.15.1.1

3lj9, 3D-view

1.15.1.1

3ljf, 3D-view

1.15.1.1

3lio, 3D-view

1.15.1.1

3lj9, 3D-view

1.15.1.1

3ljf, 3D-view

1.15.1.1

3lio, 3D-view

1.15.1.1

3lj9, 3D-view

1.15.1.1

3ljf, 3D-view

1.15.1.1

3lio, 3D-view

1.15.1.1

3lj9, 3D-view

1.15.1.1

3ljf, 3D-view

1.15.1.1

33 entries

3.1.3.24

2b1q, 3D-view

3.2.1.1

2bhy, 3D-view

3.2.1.1

2bxy, 3D-view

3.2.1.1

2bxz, 3D-view

3.2.1.1

2by0, 3D-view

3.2.1.1

2by1, 3D-view

3.2.1.1

2by2, 3D-view

3.2.1.1

2by3, 3D-view

3.2.1.1

2bhy, 3D-view

3.2.1.1

2bxy, 3D-view

3.2.1.1

2bxz, 3D-view

3.2.1.1

2by0, 3D-view

3.2.1.1

2by1, 3D-view

3.2.1.1

2by2, 3D-view

3.2.1.1

2by3, 3D-view

3.2.1.1

2bhy, 3D-view

3.2.1.1

2bxy, 3D-view

3.2.1.1

2bxz, 3D-view

3.2.1.1

2by0, 3D-view

3.2.1.1

2by1, 3D-view

3.2.1.1

2by2, 3D-view

3.2.1.1

2by3, 3D-view

3.2.1.1

2bhy, 3D-view

3.2.1.1

2bxy, 3D-view

3.2.1.1

2bxz, 3D-view

3.2.1.1

2by0, 3D-view

3.2.1.1

2by1, 3D-view

3.2.1.1

2by2, 3D-view

3.2.1.1

2by3, 3D-view

3.2.1.1

2bhy, 3D-view

3.2.1.1

2bxy, 3D-view

3.2.1.1

2bxz, 3D-view

3.2.1.1

2by0, 3D-view

3.2.1.1

2by1, 3D-view

3.2.1.1

2by2, 3D-view

3.2.1.1

2by3, 3D-view

3.2.1.1

2bhy, 3D-view

3.2.1.1

2bxy, 3D-view

3.2.1.1

2bxz, 3D-view

3.2.1.1

2by0, 3D-view

3.2.1.1

2by1, 3D-view

3.2.1.1

2by2, 3D-view

3.2.1.1

2by3, 3D-view

3.2.1.1

2bhy, 3D-view

3.2.1.1

2bxy, 3D-view

3.2.1.1

2bxz, 3D-view

3.2.1.1

2by0, 3D-view

3.2.1.1

2by1, 3D-view

3.2.1.1

2by2, 3D-view

3.2.1.1

2by3, 3D-view

3.2.1.1

2bhy, 3D-view

3.2.1.1

2bxy, 3D-view

3.2.1.1

2bxz, 3D-view

3.2.1.1

2by0, 3D-view

3.2.1.1

2by1, 3D-view

3.2.1.1

2by2, 3D-view

3.2.1.1

2by3, 3D-view

3.2.1.1

2bhy, 3D-view

3.2.1.1

2bxy, 3D-view

3.2.1.1

2bxz, 3D-view

3.2.1.1

2by0, 3D-view

3.2.1.1

2by1, 3D-view

3.2.1.1

2by2, 3D-view

3.2.1.1

2by3, 3D-view

3.2.1.1

2bhy, 3D-view

3.2.1.1

2bxy, 3D-view

3.2.1.1

2bxz, 3D-view

3.2.1.1

2by0, 3D-view

3.2.1.1

2by1, 3D-view

3.2.1.1

2by2, 3D-view

3.2.1.1

2by3, 3D-view

3.2.1.1

2bhy, 3D-view

3.2.1.1

2bxy, 3D-view

3.2.1.1

2bxz, 3D-view

3.2.1.1

2by0, 3D-view

3.2.1.1

2by1, 3D-view

3.2.1.1

2by2, 3D-view

3.2.1.1

2by3, 3D-view

3.2.1.1

77 entries

3.5.4.23

2z3g, 3D-view

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (50 results)

top print hide

Show Turnover Number (50 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.1.3.10

59.6

-

pH 7.0, 37°C

655049

2.4.1.231

0.33

-

loop 3 variant TP2, in 40 mM phosphate-citrate pH 6.2 at 45°C

720962

2.4.1.231

2.7

-

loop 3 variant TP3, in 40 mM phosphate-citrate pH 6.2 at 45°C

720962

2.4.1.231

13.3

-

30°C, pH 6.6

644900

2.4.1.231

13.3

-

30°C, pH 6.6, natural enzyme

671872

2.4.1.231

14.1

-

30°C, pH 6.6

644905

2.4.1.231

15

-

30°C, pH 6.6

644898

2.4.1.231

16.4

-

30°C, pH 6.6, recombinant enzyme

671872

2.4.1.231

18.3

-

-

674984

2.4.1.231

18.3

-

30°C, pH 6.8

674984

2.4.1.231

9 entries

2.4.1.245

1.2

-

pH 6.0, 60°C

723675

2.4.1.64

200

-

-

489326

2.4.1.64

210

-

recombinant enzyme, at pH 6.5 and 30°C

736104

2.8.2.37

0.017

-

pH 7.5, 22°C, mutant enzyme E36Q

726029

2.8.2.37

0.61

-

pH 7.5, 22°C, mutant enzyme E33A

726029

2.8.2.37

1.6

-

pH 7.5, 22°C, wild-type enzyme

726029

2.8.2.37

2.23

-

pH 7.5, 22°C

723932

3.2.1.10

5.8

-

-

690556

3.2.1.10

7.3

-

-

690556

3.2.1.20

20

-

site-specific GK24 alpha-glucosidase mutant, Vmax: 20 micromol/min mg

692850

3.2.1.20

47

-

site-specific mutant of HB27 alpha-glucosidase, Vmax: 46 micromol/min mg

692850

3.2.1.20

343

-

wild-type, Vmax: 333 micromol/min mg

692850

3.2.1.28

0.00045

-

mutant R222A/R287A, pH 7.0, 30°C

715178

3.2.1.28

0.0005

-

mutant E520A, pH 7.0, 30°C

715178

3.2.1.28

0.0009

-

mutant R287A, pH 7.0, 30°C

715178

3.2.1.28

0.12

-

mutant R169A, pH 7.0, 30°C

715178

3.2.1.28

0.18

-

mutant D322A, pH 7.0, 30°C

715178

3.2.1.28

0.3

-

mutant R222A, pH 7.0, 30°C

715178

3.2.1.28

0.67

-

-

655304

3.2.1.28

0.91

-

-

655304

3.2.1.28

0.94

-

-

655304

3.2.1.28

0.99

-

-

655304

3.2.1.28

1.02

-

-

655304

3.2.1.28

104.25

-

55°C, pH 5.0

750527

3.2.1.28

167

-

-

136154

3.2.1.28

199

-

at 37°C in sodium maleate buffer (75 mM, pH 5.5)

677555

3.2.1.28

263.25

-

wild type enzyme, 50°C, pH 6.5

750191

3.2.1.28

299.83

-

mutant enzyme Y227H, 50°C, pH 6.5

750191

3.2.1.28

319.88

-

mutant enzyme R442G, 50°C, pH 6.5

750191

3.2.1.28

433.69

-

mutant enzyme Y227H/R442G, 50°C, pH 6.0

750191

3.2.1.28

662

-

recombinant enzyme, in 50 mM sodium phosphate buffer, pH 6.6, with 0.01% Triton X-100, at 35°C

678800

3.2.1.28

730

-

wild-type, pH 7.0, 30°C

715178

5.4.99.16

61

-

pH 6.5, 30°C, recombinant mutant TSMrTt

727433

5.4.99.16

64.84

-

pH 7.0, 60°C

759527

5.4.99.16

66

-

pH 6.8, 37°C

727887

5.4.99.16

68.9

-

-

703606

5.4.99.16

68.9

-

pH 6.5, 30°C, recombinant wild-type enzyme

727433

5.4.99.16

110

-

pH 6.5, 30°C, recombinant wild-type enzyme

727433

5.4.99.16

187.6

-

45°C, pH 6.0

680282

5.4.99.16

202

-

pH 6.5, 30°C, recombinant mutant TSTtMr

727433

2.4.1.64

2 entries

2.8.2.37

4 entries

3.2.1.10

2 entries

3.2.1.20

3 entries

3.2.1.28

20 entries

5.4.99.16

8 entries

K_M Value (154 results)

top print hide

Show KM Value (154 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.3.10

7.51

-

100 mM ethanolamine buffer, pH 10.5

686372

1.1.3.10

192

-

pH 7.0, 37°C

655049

1.1.3.10

2 entries

1.1.99.29

0.05

-

0.1 M ethanolamine buffer at pH 9.4

686372

2.3.1.122

0.0083

-

-

486242

2.4.1.231

22

-

loop 3 variant TP3, in 40 mM phosphate-citrate pH 6.2 at 45°C

720962

2.4.1.231

53

-

30°C, pH 6.6, recombinant enzyme

671872

2.4.1.231

53

-

loop 3 variant TP2, in 40 mM phosphate-citrate pH 6.2 at 45°C

720962

2.4.1.231

61

-

30°C, pH 7

644907

2.4.1.231

71

-

30°C, pH 6.6, natural enzyme

671872

2.4.1.231

74.8

-

pH 7

644901

2.4.1.231

75

-

-

644903

2.4.1.231

75

-

30°C, pH 7

644902

2.4.1.231

79

-

-

674984

2.4.1.231

79

-

30°C, pH 6.8

674984

2.4.1.231

87

-

30°C, pH 6.6

644905

2.4.1.231

91

-

30°C, pH 6.6

644898

2.4.1.245

11.5

-

90°C, pH 6.5

662295

2.4.1.245

25.24

-

pH 6.0, 60°C

723675

2.4.1.245

82

-

recombinant enzyme, at 60°C

687429

2.4.1.64

0.86

-

recombinant enzyme, at pH 6.5 and 30°C

736104

2.4.1.64

0.97

-

-

489328

2.4.1.64

10

-

-

489326

2.4.1.64

33

-

-

489323

2.8.2.37

6.4

-

pH 7.5, 22°C, mutant enzyme E36Q

726029

2.8.2.37

15

-

pH 7.5, 22°C

723932

2.8.2.37

18

-

pH 7.5, 22°C, wild-type enzyme

726029

2.8.2.37

29

-

pH 7.5, 22°C, mutant enzyme E33A

726029

3.2.1.10

2

-

mutant P273G/N342T, pH 7.2, 35°C

655923

3.2.1.10

14

-

mutant P273G, pH 7.2, 35°C

655923

3.2.1.10

31.15

-

-

690556

3.2.1.10

33.6

-

-

690556

3.2.1.10

320

-

wild-type enzyme, pH 7.2, 35°C

655923

3.2.1.20

0.89

-

pH 5.5, 45°C

654259

3.2.1.20

3.2

-

-

393346

3.2.1.20

4.9

-

wild-type, Vmax: 333 micromol/min mg

692850

3.2.1.20

6.9

-

site-specific GK24 alpha-glucosidase mutant, Vmax: 20 micromol/min mg

692850

3.2.1.20

17

-

site-specific mutant of HB27 alpha-glucosidase, Vmax: 46 micromol/min mg

692850

3.2.1.28

0.0109

-

in 50 mM acetate buffer, pH 4.6, at 30°C

681784

3.2.1.28

0.031

-

pH 3.3, 37°C

710219

3.2.1.28

0.06

-

mutant R222A/R287A, pH 7.0, 30°C

715178

3.2.1.28

0.109

-

pH 6.3, 37°C

666654

3.2.1.28

0.13

-

-

136163

3.2.1.28

0.156

-

-

680519

3.2.1.28

0.16

-

signal sequenceless TreA

655983

3.2.1.28

0.16

-

at 65°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5)

679712

3.2.1.28

0.2

-

pH 4.5, 45°C

664996

3.2.1.28

0.22

-

mutant E520A, pH 7.0, 30°C

715178

3.2.1.28

0.22

-

mutant R287A, pH 7.0, 30°C

715178

3.2.1.28

0.22

-

-

136171

3.2.1.28

0.27

-

at 88°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5)

679712

3.2.1.28

0.3

-

wild-type, pH 7.0, 30°C

715178

3.2.1.28

0.31

-

periplasmic TreA

655983

3.2.1.28

0.35

-

pH 5.4, 37°C, recombinant protein with N-terminal His-tag

715680

3.2.1.28

0.3793

-

mutant enzyme Y227H/R442G, 50°C, pH 6.0

750191

3.2.1.28

0.38

-

-

656876

3.2.1.28

0.4

-

-

136155

3.2.1.28

0.41

-

at 37°C in sodium maleate buffer (75 mM, pH 5.5)

677555

3.2.1.28

0.43

-

at pH 6.0 and 30°C

729830

3.2.1.28

0.4317

-

mutant enzyme R442G, 50°C, pH 6.5

750191

3.2.1.28

0.44

-

pH 5.5, 30°C

715128

3.2.1.28

0.44

-

soluble trehalase P II, inacetate buffer, pH 5.5, at 30°C

681232

3.2.1.28

0.45

-

mutant R169A, pH 7.0, 30°C

715178

3.2.1.28

0.47

-

isozyme SfTre1

708733

3.2.1.28

0.47

-

pH 6.0, 30°C

665191

3.2.1.28

0.48

-

pH 6.5, 30°C

715128

3.2.1.28

0.5

-

pH 5.5, 30°C, immobilized enzyme

664799

3.2.1.28

0.5

-

-

136158, 136180

3.2.1.28

0.52

-

pH 7.5, 30°C

715128

3.2.1.28

0.52

-

-

136149

3.2.1.28

0.52

-

recombinant enzyme, in 50 mM sodium phosphate buffer, pH 6.6, with 0.01% Triton X-100, at 35°C

678800

3.2.1.28

0.57

-

-

136149

3.2.1.28

0.6

-

-

136179

3.2.1.28

0.63

-

presence of Ca2+

136177

3.2.1.28

0.66

-

-

655006

3.2.1.28

0.666

-

-

136153

3.2.1.28

0.67

-

-

136156

3.2.1.28

0.7194

-

mutant enzyme Y227H, 50°C, pH 6.5

750191

3.2.1.28

0.8

-

extracellular enzyme

136170

3.2.1.28

0.86

-

pH 5.5, 60°C

739003

3.2.1.28

0.89

-

-

136159

3.2.1.28

0.9

-

cell wall-bound enzyme

136170

3.2.1.28

0.99

-

wild type enzyme, 50°C, pH 6.5

750191

3.2.1.28

1

-

mutant D322A, pH 7.0, 30°C

715178

3.2.1.28

1

-

intracellular enzyme

136170

3.2.1.28

1.11

-

-

655304

3.2.1.28

1.2

-

-

136151

3.2.1.28

1.32

-

pH 5.5, 30°C, soluble enzyme

664799

3.2.1.28

1.34

-

-

655304

3.2.1.28

1.34

-

pH and temperature not specified in the publication

750787

3.2.1.28

1.37

-

-

655304

3.2.1.28

1.4

-

V-trehalase

136150

3.2.1.28

1.42

-

in 50 mM phosphate buffer, at pH 5.5 and 37°C

729110

3.2.1.28

1.47

-

55°C, pH 5.0

750527

3.2.1.28

1.5

-

cytoplsmic wild-type treF

655983

3.2.1.28

1.54

-

-

655304

3.2.1.28

1.56

-

soluble trehalase P I, in acetate buffer, pH 5.5, at 30°C

681232

3.2.1.28

1.6

-

isozyme SfTre2

708733

3.2.1.28

1.74

-

-

655304

3.2.1.28

1.77

-

-

655304

3.2.1.28

1.8

-

-

136167

3.2.1.28

1.9

-

cytoplasmic enzyme

136178

3.2.1.28

2.1

-

-

136142

3.2.1.28

2.24

-

intracellular isoform, pH 6.0, 65°C

741947

3.2.1.28

2.24

-

intracellular enzyme

136164

3.2.1.28

2.3

-

-

136176

3.2.1.28

2.3

-

in 20 mM MES buffer, pH 5.5, at 30°C

680542

3.2.1.28

2.3

-

native enzyme, in 20 mM MES, pH 6.0 for 10 min at 30°C

682673

3.2.1.28

2.6

-

recombinant enzyme, in 20 mM MES, pH 6.0 for 10 min at 30°C

682673

3.2.1.28

3.09

-

presence of Mn2+, intracellular isoform, pH 6.0, 65°C

741947

3.2.1.28

3.1

-

-

136154

3.2.1.28

3.15

-

enzyme form kidney, intestine and amniotic fluid

136143

3.2.1.28

3.3

-

detergent-solubilized trehalase

136145

3.2.1.28

3.33

-

mutant R222A, pH 7.0, 30°C

715178

3.2.1.28

3.5

-

pH 6.0

136148

3.2.1.28

3.58

-

extracellular isoform, pH 6.0, 60°C

741947

3.2.1.28

3.58

-

extracellular enzyme

136164

3.2.1.28

3.6

-

proteinase-treated trehalase

136145

3.2.1.28

3.64

-

at pH 5.5 and 55°C

731011

3.2.1.28

4.2

-

-

136146

3.2.1.28

4.7

-

-

136141, 136146

3.2.1.28

4.79

-

in absence of trehalose-c

136144

3.2.1.28

5

-

-

136165

3.2.1.28

5.28

-

in presence of trehalose-c

136144

3.2.1.28

5.4

-

-

136160

3.2.1.28

5.5

-

phosphate buffer, pH 5.7

136157

3.2.1.28

5.7

-

C-trehalase

136150

3.2.1.28

6.43

-

presence of Ca2+, intracellular isoform, pH 6.0, 65°C

741947

3.2.1.28

6.6

-

-

655271

3.2.1.28

7.63

-

presence of Mn2+, extracellular isoform, pH 6.0, 60°C

741947

3.2.1.28

8

-

actetate buffer, pH 5.0

136157

3.2.1.28

9.51

-

presence of Ca2+, extracellular isoform, pH 6.0, 60°C

741947

3.2.1.28

10

-

apparent value, at pH 7.1

677711

3.2.1.28

11

-

actetate buffer, pH 5.7

136157

3.2.1.28

11.78

-

-

656417

3.2.1.28

18

-

pH 7.5, 37°C, recombinant enzyme

704913

3.2.1.28

20

-

at 37°C

679765

3.2.1.28

42

-

pH 7.0, 30°C

701068

3.2.1.28

55

-

trehalase from activated spores

136161

3.2.1.28

72.8

-

55°C, pH 7.4

751266, 754350

5.4.99.16

0.087

-

pH 6.8, 37°C

727887

5.4.99.16

26

-

-

3493

5.4.99.16

62

-

recombinant His6-tagged wild-type enzyme, pH 6.5, 50°C

747681

5.4.99.16

69.6

-

pH 6.5, 30°C, recombinant mutant TSMrTt

727433

5.4.99.16

73.2

-

pH 7.0, 60°C

759527

5.4.99.16

75

-

in 50 mM phosphate buffer (pH 6) at 37°C

705534

5.4.99.16

82.2

-

pH 6.5, 30°C, recombinant wild-type enzyme

727433

5.4.99.16

90

-

in 40 mM potassium phosphate buffer (pH 6.8), at 37°C

692302

5.4.99.16

98.9

-

-

703606

5.4.99.16

98.9

-

pH 6.5, 30°C, recombinant wild-type enzyme

727433

5.4.99.16

154

-

pH 6.5, 30°C, recombinant mutant TSTtMr

727433

5.4.99.16

158

-

-

3491

5.4.99.16

210.3

-

45°C, pH 6.0

680282

5.4.99.16

456

-

recombinant His6-tagged mutant DM2, pH 6.5, 50°C

747681

5.4.99.16

1551

-

recombinant His6-tagged mutant DM1, pH 6.5, 50°C

747681

2.4.1.231

12 entries

2.4.1.245

3 entries

2.4.1.64

4 entries

2.8.2.37

4 entries

3.2.1.10

5 entries

3.2.1.20

5 entries

3.2.1.28

102 entries

5.4.99.16

15 entries

K_i Value (3 results)

top print hide

Show KI Value (3 entries)

EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.4.1.64

95

-

recombinant enzyme, at pH 6.5 and 30°C

736104

3.1.3.12

10

-

Ki above 10 mM, wild type enzyme, at pH 7.5 and 22°C

730793

3.1.3.24

26

-

-

682788

References & Links

Literature References (252)

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Show Reference (252 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

3489

27281143

Purification and characterization of a thermostable trehalose synthase from Thermus aquaticus

1996

Nishimoto, T.; Nakada, T.; Chaen, H.; Fukuda, S.; Sugimoto, T.; Kurimoto, M.; Tsujisaka, Y.

Biosci. Biotechnol. Biochem.

60

835-839

3490

8829531

Purification and properties of a novel enzyme, trehalose synthase, from Pimelobacter sp. R48

1996

Nishimoto, T.; Nakano, M.; Nakada, T.; Chaen, H.; Fukuda, S.; Sugimoto, T.; Kurimoto, M.; Tsujisaka, Y.

Biosci. Biotechnol. Biochem.

60

640-644

3491

28862556

Action of a thermostable trehalose synthase from Thermus aquaticus on sucrose

1997

Nishimoto, T.; Nakada, T.; Chaen, H.; Fukuda, S.; Sugimoto, T.; Kurimoto, M.; Tsujisaka, Y.

Biosci. Biotechnol. Biochem.

61

898-899

3493

-

Purification and properties of trehalose-synthesizing enzyme from Pseudomonas sp. F1

1997

Ohguchi, M.; Kubota, N.; Wada, T.; Yoshinaga, K.; Uritani, M.; Yagisawa, M.; Ohishi, K.; Yamagishi, M.; Ohta, T.; Ishikawa, K.

J. Ferment. Bioeng.

84

358-360

26155

9063974

Purification and characterization of thermostable maltooligosyl trehalose trehalohydrolase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

1996

Nakada, T.; Ikegami, S.; Chaen, H.; Kubota, M.; Fukuda, S.; Sugimoto, T.; Kurimoto, M.; Tsujisaka, Y.

Biosci. Biotechnol. Biochem.

60

267-270

134586

4327508

Partial purification and properties of a highly specific trehalose phosphate phosphatase from Mycobacterium smegmatis

1971

Matula, M.; Mitchell, M.; Elbein, A.D.

J. Bacteriol.

107

217-222

134587

4326209

Interactions among sites responsible for trehalose 6-phosphate and trehalose-activated glucose 6-phosphate hydrolysis on trehalose phosphatase isolated from Phormia regina

1971

Friedman, S.

J. Biol. Chem.

246

4122-4130

134588

-

Trehalose-6-phosphate phosphatase from insects

1966

Friedman, S.

Methods Enzymol.

8

372-374

134589

8809779

The orlA gene from Aspergillus nidulans encodes a trehalose-6-phosphate phosphatase necessary for normal growth and chitin synthesis at elevated temperatures

1996

Borgia, P.T.; Miao, Y.; Dodge, C.L.

Mol. Microbiol.

20

1287-1296

134590

1849964

Trehalose-6-phosphate synthase/phosphatase complex from bakers' yeast: purification of a proteolytically activated form

1991

Londesborough, J.; Vuorio, O.

J. Gen. Microbiol.

137

323-330

134591

1662849

The control of trehalose biosynthesis in Saccharomyces cerevisiae: evidence for a catabolite inactivation and repression of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase

1991

Francois, J.; Neves, M.J.; Hers, H.G.

Yeast

7

575-587

134592

16664852

Enzymes of alpha,alpha-trehalose metabolism in soybean nodules

1986

Salminen, S.O.; Streeter, J.G.

Plant Physiol.

81

538-541

134593

-

A temperature-sensitive mutant of Saccharomyces cerevisiae defective in the specific phosphatase of trehalose biosynthesis

1988

Piper, P.W.; Lockheart, A.

FEMS Microbiol. Lett.

49

245-250

134594

13825148

Occurence of trehalose-6-phosphatase in Phormia regina Meig.

1960

Friedman, S.

Arch. Biochem. Biophys.

88

339-343

136099

-

Purification and characterization of acid invertase from cultured tobacco cells

1988

Nakamura, M.; Hagimori, M.; Matsumoto, T.

Agric. Biol. Chem.

52

3157-3158

136100

-

Acid invertase from Tropaeolum leaves

1988

Isla, M.I.; Vattuone, M.A.; Gutierrez, M.I.; Sampietro, A.R.

Phytochemistry

27

1993-1998

136127

-

Characterization and localization of three soluble invertase forms from Lilium longiflorum flower buds

1994

Miller, W.B.; Ranwala, A.P.

Physiol. Plant.

92

247-253

136141

3286651

Purification and characterization of acid trehalase from the yeast suc2 mutant [published erratum appears in J Biol Chem 1988 Nov 5; 263(31):16512]

1988

Mittenbuehler, K.; Holzer, H.

J. Biol. Chem.

263

8537-8543

136142

3592657

Purification and properties of detergent-solubilized pig kidney trehalase

1987

Yoneyama, Y.

Arch. Biochem. Biophys.

255

168-175

136143

3828380

Intestinal and renal origin of trehalase activity in rabbit amniotic fluid

1987

Morin, P.R.; Potier, M.

Biochim. Biophys. Acta

923

371-380

136144

3024571

Partial purification and characterization of the interconvertible forms of trehalase from Saccharomyces cerevisiae

1986

Dellamora-Ortiz, G.M.; Ortiz, C.H.D.; Maia, J.C.C.; Panek, A.D.

Arch. Biochem. Biophys.

251

205-214

136145

3697377

Purification and characterization of amphiphilic trehalase from rabbit small intestine

1986

Yokota, K.; Nishi, Y.; Takesue, Y.

Biochim. Biophys. Acta

881

405-414

136146

-

Purification and properties of trehalase in Frankia ArI3

1985

Lopez, M.F.; Torrey, J.G.

Arch. Microbiol.

143

209-215

136147

2411719

Isolation and characterization of four forms of trehalase from rabbit kidney cortex

1985

Nakano, M.; Sacktor, B.

J. Biochem.

97

1329-1335

136148

6466686

Purification and characterization of kidney and intestinal brush-border membrane trehalases from the rabbit

1984

Galand, G.

Biochim. Biophys. Acta

789

10-19

136149

10564806

A comparison of thermal characteristics and kinetic parameters of trehalase from a thermophilic and a mesophilic fungus

1999

Bharadwaj, G.; Maheshwari, R.

FEMS Microbiol. Lett.

181

187-193

136150

6430270

Characterization of two trehalases in baker's yeast

1984

Londesborough, J.; Varimo, K.

Biochem. J.

219

511-518

136151

6847202

Trehalase from the cellular slime mold Dictyostelium discoideum: purification and characterization of the homogeneous enzyme from myxamoebae

1983

Killick, K.A.

Arch. Biochem. Biophys.

222

561-573

136152

6954495

Changes in the activity and properties of trehalase during early germination of yeast ascospores: correlation with trehalose breakdown as studied by in vivo 13C NMR

1982

Thevelein, J.M.; den Hollander, J.A.; Shulman, R.C.

Proc. Natl. Acad. Sci. USA

79

3503-3507

136153

6766684

Electrophoretic purification of soluble trehalase from Drosophila melanogaster by selective unstacking

1980

Bargiello, T.A.; Grossfield, J.

Anal. Biochem.

101

131-137

136154

31976

alpha,alpha-Trehalase of Trichoderma reesei

1978

Vijayakumar, P.; Ross, W.; Reese, E.T.

Can. J. Microbiol.

24

1280-1283

136155

356886

Purification and properties of trehalase from the thermophilic fungus Humicola lanuginosa

1978

Prasad, A.R.S.; Maheshwari, R.

Biochim. Biophys. Acta

525

162-170

136156

656440

Physical properties and Tris inhibition of an insect trehalase and a thermodynamic approach to the nature of its active site

1978

Terra, W.R.; Ferreira, C.; De Bianchi, A.G.

Biochim. Biophys. Acta

524

131-141

136157

18444

Purification and properties of trehalase from rat intestinal mucosal cells

1977

Nakano, M.; Sumi, Y.; Miyakawa, M.

J. Biochem.

81

1041-1049

136158

782286

A purification of trehalase from Saccharomyces cerevisiae

1976

Kelly, P.J.; Catley, B.J.

Anal. Biochem.

72

353-358

136159

1144

Properties of a free and a solubilized form of bound alpha,alpha-trehalase purified from honey bee thorax

1975

Talbot, B.G.; Muir, J.G.; Huber, R.E.

Can. J. Biochem.

53

1106-1117

136160

240424

Purification and properties of alpha,alpha-trehalase from the mucosa of rat small intestine

1975

Sasajima, K.; Kawachi, T.; Sato, S.; Sugimura, T.

Biochim. Biophys. Acta

403

139-146

136161

1138912

Some properties of trehalase from Phycomyces blakesleeanus

1975

van Assche, J.A.; Carlier, A.R.

Biochim. Biophys. Acta

391

154-161

136162

235257

Partial purification of the protein system controlling the breakdown of trehalose in baker's yeast

1975

Van Solingen, P.; van der Plaat, J.B.

Biochem. Biophys. Res. Commun.

62

553-560

136163

13525386

The purification and properties of trehalase

1958

Kalf, G.F.; Rieder, S.V.

J. Biol. Chem.

230

691-698

136164

8980633

Characterization of trehalase activities from the thermophilic fungus Scytalidium thermophilum

1996

Kadowaki, M.K.; de Lourdes Polizeli, M.; Terenzi, H.F.; Jorge, J.A.

Biochim. Biophys. Acta

1291

199-205

136165

8645714

Characterization of an acid trehalase of Saccharomyces cerevisiae present in trehalase-sucrase aggregate

1996

Biswas, N.; Ghosh, A.K.

Biochim. Biophys. Acta

1290

95-100

136166

9242988

Trehalase of Dictyostelium discoideum: inhibition by amino-containing analogs of trehalose and affinity purification

1997

Temesvari, L.A.; Cotter, D.A.

Biochimie

79

229-239

136167

1482405

Characterization of trehalase in Rhodotorula rubra

1992

Mansure, J.J.; Silva, J.T.; Panek, A.D.

Biochem. Int.

28

693-700

136168

2335220

Time-dependent inhibition of porcine kidney trehalase by aminosugars

1990

Mohd, H.; Honek, S.; Honek, J.F.

FEBS Lett.

262

359-362

136169

7519445

Molecular characterization of ovary trehalase of the silkworm, Bombyx mori and its transcriptional activation by diapause hormone

1994

Su, Z.H.; Ikeda, M.; Sato, Y.; Saito, H.; Imai, K.; Isobe, M.; Yamashita, O.

Biochim. Biophys. Acta

1218

366-374

136170

-

Purification and characterization of trehalase from the yeast Arxula adeninivorans

1992

Buettner, R.; Birnbaum, D.

Zentralbl. Mikrobiol.

147

291-296

136171

-

Purification and properties of membrane-bound trehalase from silkworm eggs

1991

Egorova, T.A.; Khomidov, K.S.

Biokhimiya

56

1374-1384

136172

8773341

Human trehalase: characterization, localization, and its increase in urine by renal proximal tubular damage

1996

Sasai-Takedatsu, M.; Taketani, S.; Nagata, N.; Furukawa, T.; Tokunaga, R.; Kojima, T.; Kobayashi, Y.

Nephron

73

179-185

136173

1710760

Osmotic induction of the periplasmic trehalase in Escherichia coli K12: characterization of the treA gene promoter

1991

Repoila, F.; Gutierrez, C.

Mol. Microbiol.

5

747-755

136174

2048932

Characterization of different forms of yeast acid trehalase in the secretory pathway

1991

Mittenbuehler, K.; Holzer, H.

Arch. Microbiol.

155

217-220

136175

8764988

Purification and biochemical characterization of the ATH1 gene product vacuolar acid trehalase, from Saccharomyces cerevisiae

1996

Alizadeh, P.; Klionsky, D.J.

FEBS Lett.

391

273-278

136176

2223824

Purification and properties of an extracellular conidial trehalase from Humicola grisea var. thermoidea

1990

Zimmermann, A.L.S.; Terenzi, H.F.; Jorge, J.A.

Biochim. Biophys. Acta

1036

41-46

136177

-

Biochemical characterization of a Ca2+-dependent acid trehalase activity from the thermophilic fungus Chaetomium thermophilum var. coprophilum

1999

De Almeida, E.M.; de Lourdes Polizeli, M.; Terenzi, H.F.; Jorge, J.A.

FEMS Microbiol. Lett.

171

11-15

136178

8892826

Characterization of a cytoplasmic trehalase of Escherichia coli

1996

Horlacher, R.; Uhland, K.; Klein, W.; Ehrmann, M.; Boos, W.

J. Bacteriol.

178

6250-6257

136179

7765274

Purification and some properties of a trehalase from a green alga, Lobosphaera sp.

1994

Nakano, H.; Moriwaki, M.; Washino, T.; Kino, T.; Yoshizumi, H.; Kitahara, S.

Biosci. Biotechnol. Biochem.

58

1430-1434

136180

-

Degradation of the compatible solute trehalose in Ectothiorhodospira halochloris: isolation and characterization of trehalase

1990

Herzog, R.M.; Galinski, E.A.; Trueper, H.G.

Arch. Microbiol.

153

600-606

136199

6434506

Fungal glucoamylases

1983

Manjunath, P.; Shenoy, B.C.; Raghavendra Roa, M.R.

J. Appl. Biochem.

5

235-260

136228

9521694

Enzymatic properties of the cysteinesulfinic acid derivative of the catalytic-base mutant Glu400->Cys of glucoamylase from Aspergillus awamori

1998

Fierobe, H.P.; Clarke, A.J.; Tull, D.; Svensson, B.

Biochemistry

37

3753-3759

136844

2119579

Mode of action and substrate specificity of a purified exo-1,4-beta-D-glucosidase cloned from the cellulolytic bacterium Ruminococcus albus AR67

1990

Ware, C.E.; Lachke, A.H.; Gregg, K.

Biochem. Biophys. Res. Commun.

171

777-786

137024

-

Enzymatic preparation of novel non-reducing oligosaccharides having an isomaltosyl residue by using the transfer action of isomaltodextranase from Arthrobacter globiformis T6

1995

Kim, Y.K.; Tsumuraya, Y.; Sakano, Y.

Biosci. Biotechnol. Biochem.

59

1367-1369

171187

4561015

Biochemistry of alpha-galactosidases

1972

Dey, P.M.; Pridham, J.B.

Adv. Enzymol. Relat. Areas Mol. Biol.

36

91-130

326283

8987723

Properties of an intracellular beta-glucosidase purified from the cellobiose-fermenting yeast Candida wickerhamii

1996

Skory, C.D.; Freer, S.N.; Bothast, R.J.

Appl. Microbiol. Biotechnol.

546

353-359

326295

8486554

Purification and properties of the endocellular beta-glucosidase from Candida cacaoi Buckley and Van Uhden CBS 2020

1993

Drider, D.; Pommares, P.; Chemardin, P.; Arnaud, A.; Galzy, P.

J. Appl. Bacteriol.

74

473-479

389892

-

Purification und properties of 3-ketosucrose-forming enzyme from the cells of Agrobacterium tumefaciens

1967

Hayano, K.; Fukui, S.

J. Biol. Chem.

242

3665-3672

389893

165382

Hexopyranoside: cytochrome c oxidoreductase from Agrobacterium

1975

Van Beeumen, J.; De Ley, J.

Methods Enzymol.

41

153-158

389895

3818559

Purification and properties of glucoside 3-dehydrogenase from Flavobacterium saccharophilum

1986

Takeuchi, M.; Ninomiya, K.; Kawabata, K.; Asano, N.; Kameda, Y.; Matsui, K.

J. Biochem.

100

1049-1055

389896

-

Purification and properties of soluble D-glucoside 3-dehydrogenase from Flavobacterium saccharophilum

1988

Takeuchi, M.; Asano, N.; Kameda, Y.;Matsui, K.

Agric. Biol. Chem.

52

1905-1912

389900

9210346

3-Ketoglycoside-mediated metabolism of sucrose in E. coli as conferred by genes from Agrobacterium tumefaciens

1997

Schuerman, P.L.; Liu, J.S.; Mou, H.; Dandekar, A.M.

Appl. Microbiol. Biotechnol.

47

560-565

393325

9285074

Purification and biochemical characterisation of human placental acid alpha-glucosidase

1997

Chadalavada, D.M.; Sivakami, S.

Biochem. Mol. Biol. Int.

42

1051-1061

393333

10388682

Purification and characterization of an alpha-glucosidase from Rhizobium sp. (Robinia pseudoacacia L.) strain USDA 4280

1999

Berthelot, K.; Delmotte, F.M.

Appl. Environ. Microbiol.

65

2907-2911

393334

-

Characterization of high pI alpha-glucosidase from germinated barley seeds: substrate specificity subsite affinities and active-site residues

1995

Im, H.; Henson, C.A.

Carbohydr. Res.

277

145-159

393344

10648165

Overexpression, purification, and characterization of a barley alpha-glucosidase secreted by Pichia pastoris

2000

Muslin, E.H.; Kanikula, A.M.; Clark, S.E.; Henson, C.A.

Protein Expr. Purif.

18

20-26

393346

7764968

Purification and characterization of a Bacillus sp. SAM1606 thermostable alpha-glucosidase with transglucosylation activity

1994

Nakao, M.; Nakayama, T.; Harada, M.; Kakudo, A.; Ikemoto, H.; Kobayashi, S.; Shibano, Y.

Appl. Microbiol. Biotechnol.

41

337-343

393349

-

Production and characteristics of an intracellular alpha-glucosidase from a color variant strain of Aureobasidium pullulans

1993

Saha, B.C.; Bothast, R.J.

Curr. Microbiol.

27

73-77

486242

3654621

Purification and characterization of a novel mycolic acid exchange enzyme from Mycobacterium smegmatis

1987

Sathyamoorthy, N.; Takayama, K.

J. Biol. Chem.

262

13417-13423

488230

-

Isolation and partial characterization of three forms of glycogen phosphorylase from fat body of Locusta migratoria

1988

Van Marrewijk, W.J.A.; Van den Broek, A.T.M.; Beenakkers, A.M.T.

Insect Biochem.

18

37-44

488594

8480996

Biosynthesis and function of trehalose in Ectothiorhodospira halochloris

1993

Lippert, K.; Galinski, E.A.; Trueper, H.G.

Antonie van Leeuwenhoek

63

85-91

488599

14285483

The enzymes of glycogen and trehalose synthesis in silk moth fat body

1965

Murphy, T.A.; Wyatt, G.R.

J. Biol. Chem.

240

1500-1508

488614

10831428

Characterization of a bifunctional enzyme fusion of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Escherichia coli

2000

Seo, H.S.; Koo, Y.J.; Lim, J.Y.; Song, J.T.; Kim, C.H.; Kim, J.K.; Lee, J.S.; Choi, Y.D.

Appl. Environ. Microbiol.

66

2484-2490

489320

5413942

Trehalose phosphorylase from Euglena gracilis

1970

Belocopitow, E.; Marechal, L.R.

Biochim. Biophys. Acta

198

151-154

489321

6236810

Fructose 2,6-bisphosphate, a potent regulator of carbohydrate metabolism, inhibits trehalose phosphorylase from protist Euglena gracilis

1984

Miyatake, K.; Kuramoto, Y.; Kitaoka, S.

Biochem. Biophys. Res. Commun.

122

906-911

489322

-

Enzymatic synthesis of trehalose from maltose

1985

Murao, S.; Nagano, H.; Ogura, S.; Nishino, T.

Agric. Biol. Chem.

49

2113-2118

489323

4623846

Metabolism of trehalose in Euglena gracilis. I. Partial purification and some properties of trehalose phosphorylase

1972

Marechal, L.R.; Belocopitow, E.

J. Biol. Chem.

247

3223-3228

489325

7557309

Production of trehalose phosphorylase by Catellatospora ferruginea

1995

Aisaka, K.; Masuda, T.

FEMS Microbiol. Lett.

131

47-51

489326

-

Purification and characterization of trehalose phosphorylase from Micrococcus varians

1995

Kizawa, H.; Miyagawa, K.I.; Sugiyama, Y.

Biosci. Biotechnol. Biochem.

59

1908-1912

489328

-

Purification and characterization of thermostable trehalose phosphorylase from Thermoanaerobium brockii

1999

Chaen, H.; Nakada, T.; Nishimoto, T.; Kuroda, N.; Fukuda, S.; Sugimoto, T.; Kurimoto, M.; Tsujisaka, Y.

J. Appl. Glycosci.

46

399-405

644544

9042751

Pyranose 2-dehydrogenase, a novel sugar oxidoreductase from the basidiomycete fungus Agaricus bisporus

1997

Volc, J.; Kubátová, E.; Wood, D.; Daniel, G.

Arch. Microbiol.

167

119-125

644547

11511865

Screening of basidiomycete fungi for the quinone-dependent sugar C-2/C-3 oxidoreductase, pyranose dehydrogenase, and properties of the enzyme from Macrolepiota rhacodes

2001

Volc, J.; Kubátová, E.; Daniel, G.; Sedmera, P.; Haltrich, D.

Arch. Microbiol.

176

178-186

644548

-

C-3 oxidation of non-reducing sugars by a fungal pyranose dehydrogenase: spectral characterization

2002

Volc, J.; Sedmera, P.; Halada, P.; Daniel, G.; Pøikyrlová, V.; Haltrich, D.

J. Mol. Catal. B

17

91-100

644897

9872418

The stereochemical course of the reaction mechanism of trehalose phosphorylase from Schizophyllum commune

1998

Eis, C.; Albert, M.; Dax, K.; Nidetzky, B.

FEBS Lett.

440

440-443

644898

10393097

Characterization of trehalose phosphorylase from Schizophyllum commune

1999

Eis, C.; Nidetzky, B.

Biochem. J.

341

385-393

644899

11931662

Substrate-binding recognition and specificity of trehalose phosphorylase from Schizophyllum commune examined in steady-state kinetic studies with deoxy and deoxyfluoro substrate analogues and inhibitors

2002

Eis, C.; Nidetzky, B.

Biochem. J.

363

335-340

644900

11389683

Fungal trehalose phosphorylase: kinetic mechanism, pH-dependence of the reaction and some structural properties of the enzyme from Schizophyllum commune

2001

Eis, C.; Watkins, M.; Prohaska, T.; Nidetzky, B.

Biochem. J.

356

757-767

644901

12880768

Cloning and characterization of a gene encoding trehalose phosphorylase (TP) from Pleurotus sajor-caju

2003

Han, S.E.; Kwon, H.B.; Lee, S.B.; Yi, B.Y.; Murayama, I.; Kitamoto, Y.; Byun, M.O.

Protein Expr. Purif.

30

194-202

644902

100302494

alpha-Glucose-1-phosphate formation by a novel trehalose phosphorylase from Flammulina velutipes

1988

Kitamoto, Y.; Akashi, H.; Tanaka, H.; Mori, N.

FEMS Microbiol. Lett.

55

147-149

644903

-

Purification and properties of alpha-glucose 1-phosphate-forming trehalose phosphorylase from a basidiomycete, Pleurotus ostreatus

2000

Kitamoto, Y.; Osaki, N.; Tanaka, H.; Sasaki, H.; Mori, N.

Mycoscience

41

607-613

644904

-

Survey of alpha-glucose 1-phosphate forming trehalose phosphorylase and trehalase in various fungi including basidiomycetous mushrooms

1998

Kitamoto, Y.; Tanaka, H.; Osaki, N.

Mycoscience

39

327-331

644905

11736665

alpha-Retaining glucosyl transfer catalysed by trehalose phosphorylase from Schizophyllum commune: mechanistic evidence obtained from steady-state kinetic studies with substrate analogues and inhibitors

2001

Nidetzky, B.; Eis, C.

Biochem. J.

360

727-736

644906

9797287

Purification and characterization of a trehalose synthase from the basidiomycete Grifola frondosa

1998

Saito, K.; Kase, T.; Takahashi, E.; Horinouchi, S.

Appl. Environ. Microbiol.

64

4340-4345

644907

9813313

Purification and characterization of trehalose phosphorylase from the commercial mushroom Agaricus bisporus

1998

Wannet, W.J.; Op den Camp, H.J.; Wisselink, H.W.; van der Drift, C.; Van Griensven, L.J.; Vogels, G.D.

Biochim. Biophys. Acta

1425

177-188

649392

14711665

Overproduction of trehalose: heterologous expression of Escherichia coli trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in Corynebacterium glutamicum

2004

Padilla, L.; Kramer, R.; Stephanopoulos, G.; Agosin, E.

Appl. Environ. Microbiol.

70

370-376

649561

11162529

Directed Evolution of Operon of Trehalose-6-phosphate Synthase/Phosphatase from Escherichia coli

2001

Kong, X.; Liu, Y.; Gou, X.; Zhang, H.; Wang, X.; Zhang, J.

Biochem. Biophys. Res. Commun.

280

396-400

651386

12748054

Cloning and characterization of genes encoding trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2) from Zygosaccharomyces rouxii

2003

Kwon, H.B.; Yeo, E.T.; Hahn, S.E.; Bae, S.C.; Kim, D.Y.; Byun, M.O.

FEMS Yeast Res.

3

433-440

651481

11895938

Disruption of the Candida albicans TPS2 gene encoding trehalose-6-phosphate phosphatase decreases infectivity without affecting hypha formation

2002

Van Dijck, P.; De Rop, L.; Szlufcik, K.; Van Ael, E.; Thevelein, J.M.

Infect. Immun.

70

1772-1782

651667

11004189

Characterization of tpp1(+) as encoding a main trehalose-6P phosphatase in the fission yeast Schizosaccharomyces pombe

2000

Franco, A.; Soto, T.; Vicente-Soler, J.; Guillen, P.V.; Cansado, J.; Gacto, M.

J. Bacteriol.

182

5880-5884

652396

12417583

Purification, cloning, expression, and properties of mycobacterial trehalose-phosphate phosphatase

2003

Klutts, S.; Pastuszak, I.; Edavana, V.K.; Thampi, P.; Pan, Y.T.; Abraham, E.C.; Carroll, J.D.; Elbein, A.D.

J. Biol. Chem.

278

2093-2100

653157

11988502

Disruption in Candida albicans of the TPS2 gene encoding trehalose-6-phosphate phosphatase affects cell integrity and decreases infectivity

2002

Zaragoza, O.; de Virgilio, C.; Ponton, J.; Gancedo, C.

Microbiology

148

1281-1290

653540

12586876

Expression of a bifunctional fusion of the Escherichia coli genes for trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth

2003

Jang, I.C.; Oh, S.J.; Seo, J.S.; Choi, W.B.; Song, S.I.; Kim, C.H.; Kim, Y.S.; Seo, H.S.; Choi, Y.D.; Nahm, B.H.; Kim, J.K.

Plant Physiol.

131

516-524

654259

11872475

Novel alpha-glucosidase from Aspergillus nidulans with strong transglycosylation activity

2002

Kato, N.; Suyama, S.; Shirokane, M.; Kato, M.; Kobayashi, T.; Tsukagoshi, N.

Appl. Environ. Microbiol.

68

1250-1256

654304

12658515

Cloning and characterization of two alpha-glucosidases from Bifidobacterium adolescentis DSM20083

2003

van den Broek, L.A.M.; Struijs, K.; Verdoes, J.C.; Beldman, G.; Voragen, A.G.J.

Appl. Microbiol. Biotechnol.

61

55-60

654532

12943532

A role for calcium in the regulation of neutral trehalase activity in the fission yeast Schizosaccharomyces pombe

2003

Franco, A.; Soto, T.; Vicente-Soler, J.; Paredes, V.; Madrid, M.; Gacto, M.; Cansado, J.

Biochem. J.

376

209-217

655006

11826961

Purification and identification of the essential ionizable groups of honeybee, Apis mellifera L., trehalase

2001

Lee, J.H.; Tsuji, M.; Nakamura, M.; Nishimoto, M.; Okuyama, M.; Mori, H.; Kimura, A.; Matsui, H.; Chiba, S.

Biosci. Biotechnol. Biochem.

65

2657-2665

655049

14730138

Purification, characterization, and molecular cloning of a pyranose oxidase from the fruit body of the basidiomycete,Tricholoma matsutake

2003

Takakura, Y.; Kuwata, S.

Biosci. Biotechnol. Biochem.

67

2598-2607

655271

12941639

Purification and characterization of acid trehalase from muscle of Ascaris suum (Nematoda)

2003

Dmitryjuk, M.; Zoltowska, K.

Comp. Biochem. Physiol. B

136

61-69

655304

15170238

Nucleotide sequence variation does not relate to differences in kinetic properties of neutral trehalase from the insect pathogenic fungus Metarhizium anisopliae

2004

Small, C.L.; Donaldson, N.; Bidochka, M.J.

Curr. Microbiol.

48

428-434

655381

-

Thermostabilization of Bacillus subtilis CCMI 966 xylanases with trehalose. Study of deactivation kinetics

2004

Sa-Pereira, P.; Carvalho, A.S.L.; Costa-Ferreira, M.; Aires-Barros, M.R.

Enzyme Microb. Technol.

34

278-282

655599

11094289

Role for trehalase during germination of spores in the fission yeast Schizosaccharomyces pombe

2000

Beltran, F.F.; Castillo, R.; Vicente-Soler, J.; Cansado, J.; Gacto, M.

FEMS Microbiol. Lett.

193

117-121

655729

-

Multi-sugar analysis system using a novel glucose-3-dehydrogenase electrode

2002

Hamafuji, T.; Takano, Y.; Tsugawa, W.; Sode, K.

Instrumentation Sci. Technol.

30

97-105

655752

15013735

Purification and biochemical characterisation of a membrane-bound alpha-glucosidase from the parasite Entamoeba histolytica

2004

Bravo-Torres, J.C.; Villagomez-Castro, J.C.; Calvo-Mendez, C.; Flores-Carreon, A.; Lopez-Romero, E.

Int. J. Parasitol.

34

455-462

655923

14607981

Deciphering the molecular basis of the broad substrate specificity of alpha-glucosidase from Bacillus sp. SAM1606

2003

Noguchi, A.; Yano, M.; Ohshima, Y.; Hemmi, H.; Inohara-ochiai, M.; Okada, M.; Min, K.s.; Nakayama, T.; Nishino, T.

J. Biochem.

134

543-550

655983

10816581

Determinants of translocation and folding of TreF, a trehalase of Escherichia coli

2000

Uhland, K.; Mondigler, M.; Spiess, C.; Prinz, W.; Ehrmann, M.

J. Biol. Chem.

275

23439-23445

656417

100167757

Partial purification and characterization of neutral trehalase from commercial bakers' yeast, Saccharomyces cerevisiae

2000

Yarar, S.; Hamamci, H.; Bakir, U.

J. Food Biochem.

24

443-451

656848

-

Purification and characterization of an extracellular acid trehalase from Lentinula edodes

2001

Murata, M.; Nagai, M.; Takao, M.; Suzuki, A.; Sakai, T.; Terashita, T.

Mycoscience

42

479-482

656876

100451756

Identification and characterization of an extracellular acid trehalase from the ectomycorrhizal fungus Amanita muscaria

2000

Wisser, G.; Guttenberger, M.; Hampp, R.; Nehls, U.

New Phytol.

146

169-175

657031

11161063

Trehalose and trehalase in Arabidopsis

2001

Müller, J.; Aeschbacher, R.A.; Wingler, A.; Boller, T.; Wiemken, A.

Plant Physiol.

125

1086-1093

662295

15364950

TreT, a novel trehalose glycosyltransferring synthase of the hyperthermophilic archaeon Thermococcus litoralis

2004

Qu, Q.; Lee, S-J.; Boos, W.

J. Biol. Chem.

279

47890-47897

664188

15809023

Biochemical characterisation of the trehalase of thermophilic fungi: an enzyme with mixed properties of neutral and acid trehalase

2005

Lucio-Eterovic, A.K.B.; Jorge, J.A.; Polizeli, M.de L.T.M.; Terenzi, H.F.

Biochim. Biophys. Acta

1723

201-207

664664

16458033

Absorption of toxic beta-glucosides produced by plants and their effects on tissue trehalase from insects

2006

Silva, M.C.P.; Terra, W.R.; Ferreira, C.

Comp. Biochem. Physiol. B

143

367-373

664799

-

A simple method for obtaining reusable reactors containing immobilized trehalase: characterization of a crude trehalase preparation immobilized on chitin particles

2006

Martins, A.S.; Peixoto, D.; Paiva, L.M.C.; Panek, A.D.; Paiva, C.L.A.

Enzyme Microb. Technol.

38

486-492

664996

16139441

Characterization of an acid trehalase produced by the thermotolerant fungus Rhizopus microsporus var. rhizopodiformis: biochemical properties and immunochemical localisation

2005

de Aquino, A.C.M.; Peixoto-Nogueira, S.C.; Jorge, J.A.; Terenzi, H.F.; Polizeli, M.de L.

FEMS Microbiol. Lett.

251

169-175

665006

15780651

Acid trehalase in yeasts and filamentous fungi: localization, regulation and physiological function

2005

Parrou, J.L.; Jules, M.; Beltran, G.; Francois, J.

FEMS Yeast Res.

5

503-511

665191

15475303

The role of carboxyl, guanidine and imidazole groups in catalysis by a midgut trehalase purified from an insect larvae

2004

Silva, M.C.P.; Terra, W.R.; Ferreira, C.

Insect Biochem. Mol. Biol.

34

1089-1099

665197

16164606

Membran-penetrating trehalase from silkworm Bombyx mori. Molecular cloning and localization in larval midgut

2005

Mitsumasu, K.; Azuma, M.; Niimi, T.; Yamashita, O.; Yaginuma, T.

Insect Mol. Biol.

14

501-508

665566

15252058

The ATC1 gene encodes a cell wall-linked acid trehalase required for growth on trehalose in Candida albicans

2004

Pedreno, Y.; Maicas, S.; Arguelles, J.C.; Sentandreu, R.; Valentin, E.

J. Biol. Chem.

279

40852-40860

666654

15907687

Physiological implications of trehalase from Phaseolus vulgaris root nodules: partial purification and characterization

2005

Garcia, N.A.T.; Iribarne, C.; Lopez, M.; Herrera-Cervera, J.A.; Lluch, C.

Plant Physiol. Biochem.

43

355-361

671872

16640506

Structure-function relationships for Schizophyllum commune trehalose phosphorylase and their implications for the catalytic mechanism of family GT-4 glycosyltransferases

2006

Goedl, C.; Griessler, R.; Schwarz, A.; Nidetzky, B.

Biochem. J.

397

491-500

672930

16872587

Construction and characterization of chimeric enzymes of kojibiose phosphorylase and trehalose phosphorylase from Thermoanaerobacter brockii

2006

Yamamoto, T.; Yamashita, H.; Mukai, K.; Watanabe, H.; Kubota, M.; Chaen, H.; Fukuda, S.

Carbohydr. Res.

341

2350-2359

674984

17222933

Trehalose phosphorylase from Pleurotus ostreatus: characterization and stabilization by covalent modification, and application for the synthesis of alpha,alpha-trehalose

2007

Schwarz, A.; Goedl, C.; Minani, A.; Nidetzky, B.

J. Biotechnol.

129

140-150

675940

15786733

Opposing effects of two osmolytes - trehalose and glycerol--on thermal inactivation of rabbit muscle 6-phosphofructo-1-kinase

2005

Faber-Barata, J.; Sola-Penna, M.

Mol. Cell. Biochem.

269

203-207

677555

17455176

Molecular basis for trehalase inhibition revealed by the structure of trehalase in complex with potent inhibitors

2007

Gibson, R.P.; Gloster, T.M.; Roberts, S.; Warren, R.A.; Storch de Gracia, I.; Garcia, A.; Chiara, J.L.; Davies, G.J.

Angew. Chem.

46

4115-4119

677711

18065618

New insights into trehalose metabolism by Saccharomyces cerevisiae: NTH2 encodes a functional cytosolic trehalase, and deletion of TPS1 reveals Ath1p-dependent trehalose mobilization

2008

Jules, M.; Beltran, G.; Francois, J.; Parrou, J.L.

Appl. Environ. Microbiol.

74

605-614

678800

17827701

Molecular cloning of cDNA for trehalase from the European honeybee, Apis mellifera L., and its heterologous expression in Pichia pastoris

2007

Lee, J.H.; Saito, S.; Mori, H.; Nishimoto, M.; Okuyama, M.; Kim, D.; Wongchawalit, J.; Kimura, A.; Chiba, S.

Biosci. Biotechnol. Biochem.

71

2256-2265

678895

17899389

Co-purification of glucanase with acid trehalase-invertase aggregate in Saccharomyces cerevisiae

2008

Basu, A.; Chaudhuri, P.; Malakar, D.; Ghosh, A.K.

Biotechnol. Lett.

30

299-304

679712

16944251

A highly thermostable trehalase from the thermophilic bacterium Rhodothermus marinus

2007

Jorge, C.D.; Sampaio, M.M.; Hreggvidsson, G.O.; Kristjanson, J.K.; Santos, H.

Extremophiles

11

115-122

679765

17319935

A novel trehalase from Mycobacterium smegmatis - purification, properties, requirements

2007

Carroll, J.D.; Pastuszak, I.; Edavana, V.K.; Pan, Y.T.; Elbein, A.D.

FEBS J.

274

1701-1714

679865

17673210

The Arabidopsis thaliana trehalase is a plasma membrane-bound enzyme with extracellular activity

2007

Frison, M.; Parrou, J.L.; Guillaumot, D.; Masquelier, D.; Francois, J.; Chaumont, F.; Batoko, H.

FEBS Lett.

581

4010-4016

679927

17916074

Role of 14-3-3 proteins in the regulation of neutral trehalase in the yeast Saccharomyces cerevisiae

2008

Panni, S.; Landgraf, C.; Volkmer-Engert, R.; Cesareni, G.; Castagnoli, L.

FEMS Yeast Res.

8

53-63

680282

16968068

Gene cloning, expression, and biochemical characterization of a recombinant trehalose synthase from Picrophilus torridus in Escherichia coli

2006

Chen, Y.S.; Lee, G.C.; Shaw, J.F.

J. Agric. Food Chem.

54

7098-7104

680519

18192391

Role of periplasmic trehalase in uptake of trehalose by the thermophilic bacterium Rhodothermus marinus

2008

Jorge, C.D.; Fonseca, L.L.; Boos, W.; Santos, H.

J. Bacteriol.

190

1871-1878

680542

16980715

Identification of an extracellular acid trehalase and its gene involved in fungal pathogenesis of Metarhizium anisopliae

2006

Zhao, H.; Charnley, A.K.; Wang, Z.; Yin, Y.; Li, Z.; Li, Y.; Cao, Y.; Peng, G.; Xia, Y.

J. Biochem.

140

319-327

680813

17650500

Osmosensing properties of the histidine protein kinase MtrB from Corynebacterium glutamicum

2007

Moeker, N.; Reihlen, P.; Kraemer, R.; Morbach, S.

J. Biol. Chem.

282

27666-27677

681232

-

Purification and properties of two types of soluble trehalases from embryonic larvae of the silkworm, Bombyx mori

2006

Huang, J.; Furusawa, T.; Sadakane, K.; Sugimura, Y.

J. Insect Biotechnol. Sericol.

75

1-8

681784

17428646

Solubilization and characterization of a cell wall-bound trehalase from ascospores of the fission yeast Schizosaccharomyces pombe

2007

Vicente-Soler, J.; Soto, T.; Madrid, M.; Nunez, A.; Cansado, J.; Gacto, M.

Microbiol. Res.

164

304-311

681792

17464051

Disruption of the Candida albicans ATC1 gene encoding a cell-linked acid trehalase decreases hypha formation and infectivity without affecting resistance to oxidative stress

2007

Pedreno, Y.; Gonzalez-Parraga, P.; Martinez-Esparza, M.; Sentandreu, R.; Valentin, E.; Argueelles, J.C.

Microbiology

153

1372-1381

682056

17512710

Isoforms of trehalase and invertase of Fusarium oxysporum

2007

Wolska-Mitaszko, B.; Jaroszuk-Scisei, J.; Pszeniczna, K.

Mycol. Res.

111

456-465

682142

17504469

Trehalose turnover during abiotic stress in arbuscular mycorrhizal fungi

2007

Ocon, A.; Hampp, R.; Requena, N.

New Phytol.

174

879-891

682602

-

A novel alpha-glucosidase from Chaetomium thermophilum var. coprophilum that converts maltose into trehalose: Purification and partial characterisation of the enzyme

2006

Giannesi, G.C.; Polizeli, M.de L.; Terenzi, H.F.; Jorge, J.A.

Process Biochem.

41

1729-1735

682673

17419071

Expression, purification, and characterization of recombinant Metarhizium anisopliae acid trehalase in Pichia pastoris

2007

Liu, Y.; Wang, Z.; Yin, Y.; Cao, Y.; Zhao, H.; Xia, Y.

Protein Expr. Purif.

54

66-72

682699

17203393

Thermal destabilization of stem bromelain by trehalose

2007

Habib, S.; Khan, M.A.; Younus, H.

Protein J.

26

117-124

682788

17510968

Crystal structure of a cyanobacterial sucrose-phosphatase in complex with glucose-containing disaccharides

2007

Fieulaine, S.; Lunn, J.E.; Ferrer, J.

Proteins Struct. Funct. Bioinform.

68

796-801

686372

-

Amperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodes

2007

Tasca, F.; Timur, S.; Ludwig, R.; Haltrich, D.; Volc, J.; Antiochia, R.; Gorton, L.

Electroanalysis

19

294-302

687429

18835983

A unique combination of genetic systems for the synthesis of trehalose in Rubrobacter xylanophilus: properties of a rare actinobacterial TreT

2008

Nobre, A.; Alarico, S.; Fernandes, C.; Empadinhas, N.; da Costa, M.S.

J. Bacteriol.

190

7939-7946

690556

19114534

Characterization of two novel alpha-glucosidases from Bifidobacterium breve UCC2003

2009

Pokusaeva, K.; OConnell-Motherway, M.; Zomer, A.; Fitzgerald, G.F.; van Sinderen, D.

Appl. Environ. Microbiol.

75

1135-1143

691645

18281024

Studying non-covalent enzyme carbohydrate interactions by STD NMR

2008

Brecker, L.; Schwarz, A.; Goedl, C.; Kratzer, R.; Tyl, C.E.; Nidetzky, B.

Carbohydr. Res.

343

2153-2161

692302

18505459

Trehalose synthase converts glycogen to trehalose

2008

Pan, Y.T.; Carroll, J.D.; Asano, N.; Pastuszak, I.; Edavana, V.K.; Elbein, A.D.

FEBS J.

275

3408-3420

692323

18205830

The phosphate site of trehalose phosphorylase from Schizophyllum commune probed by site-directed mutagenesis and chemical rescue studies

2008

Goedl, C.; Nidetzky, B.

FEBS J.

275

903-913

692850

18223075

Molecular and physiological role of the trehalose-hydrolyzing alpha-glucosidase from Thermus thermophilus HB27

2008

Alarico, S.; da Costa, M.S.; Empadinhas, N.

J. Bacteriol.

190

2298-2305

693804

18314671

Trehalose catabolism enzymes in L3 and L4 larvae of Anisakis simplex

2007

Lopienska-Biernat, E.; Zoltowska, K.; Rokicki, J.

J. Parasitol.

93

1291-1294

695788

-

Pyranose dehydrogenases: biochemical features and perspectives of technological applications

2009

Peterbauer, C.; Volc, J.

Appl. Microbiol. Biotechnol.

146

491-499

696962

19284595

An optimised system for refolding of human glucose 6-phosphate dehydrogenase

2009

Wang, X.; Engel, P.

BMC Biotechnol.

9

19

699078

19433216

Trehalose-mediated thermal stabilization of glucose oxidase from Aspergillus niger

2009

Paz-Alfaro, K.J.; Ruiz-Granados, Y.G.; Uribe-Carvajal, S.; Sampedro, J.G.

J. Biotechnol.

141

130-136

699548

18586271

Structural insights into the substrate specificity and function of Escherichia coli K12 YgjK, a glucosidase belonging to the glycoside hydrolase family 63

2008

Kurakata, Y.; Uechi, A.; Yoshida, H.; Kamitori, S.; Sakano, Y.; Nishikawa, A.; Tonozuka, T.

J. Mol. Biol.

381

116-128

701068

19073263

Heterologous expression in Escherichia coli of Neurospora crassa neutral trehalase as an active enzyme

2009

de Almeida, F.M.; Bonini, B.M.; Beton, D.; Jorge, J.A.; Terenzi, H.F.; da Silva, A.M.

Protein Expr. Purif.

65

185-189

703606

19779856

Overexpression and characterization of a thermostable trehalose synthase from Meiothermus ruber

2010

Zhu, Y.; Wei, D.; Zhang, J.; Wang, Y.; Xu, H.; Xing, L.; Li, M.

Extremophiles

14

1-8

704913

19436130

Water-stress induced trehalose accumulation and control of trehalase in the cyanobacterium Nostoc punctiforme IAM M-15

2009

Yoshida, T.; Sakamoto, T.

J. Gen. Appl. Microbiol.

55

135-145

705534

19523196

Molecular cloning and expression of a novel trehalose synthase gene from Enterobacter hormaechei

2009

Yue, M.; Wu, X.L.; Gong, W.N.; Ding, H.B.

Microb. Cell Fact.

8

34

707785

19897915

Catalytic reaction mechanism based on alpha-secondary deuterium isotope effects in hydrolysis of trehalose by European honeybee trehalase

2009

Mori, H.; Lee, J.H.; Okuyama, M.; Nishimoto, M.; Ohguchi, M.; Kim, D.; Kimura, A.; Chiba, S.

Biosci. Biotechnol. Biochem.

73

2466-2473

708486

19703229

The Saccharomyces cerevisiae vacuolar acid trehalase is targeted at the cell surface for its physiological function

2009

He, S.; Bystricky, K.; Leon, S.; Francois, J.M.; Parrou, J.L.

FEBS J.

276

5432-5446

708733

19843188

Sequencing of Spodoptera frugiperda midgut trehalases and demonstration of secretion of soluble trehalase by midgut columnar cells

2009

Silva, M.C.; Ribeiro, A.F.; Terra, W.R.; Ferreira, C.

Insect Mol. Biol.

18

769-784

709400

19615372

Characterization of putative soluble and membrane-bound trehalases in a hemipteran insect, Nilaparvata lugens

2009

Gu, J.; Shao, Y.; Zhang, C.; Liu, Z.; Zhang, Y.

J. Insect Physiol.

55

997-1002

709841

19520725

The three trehalases Nth1p, Nth2p and Ath1p participate in the mobilization of intracellular trehalose required for recovery from saline stress in Saccharomyces cerevisiae

2009

Garre, E.; Matallana, E.

Microbiology

155

3092-3099

710219

-

Inhibitory effects of validamycin compounds on the termites trehalase

2009

Jin, L.; Zheng, Y.

Pestic. Biochem. Physiol.

95

28-32

710386

20405036

Different functions of the insect soluble and membrane-bound trehalase genes in chitin biosynthesis revealed by RNA interference

2010

Chen, J.; Tang, B.; Chen, H.; Yao, Q.; Huang, X.; Chen, J.; Zhang, D.; Zhang, W.

PLoS ONE

5

e10133

713777

21927828

Purification and partial biochemical characterization of a membrane-bound type II-like alpha-glucosidase from the yeast morphotype of Sporothrix schenckii

2012

Torres-Rodriguez, B.I.; Flores-Berrout, K.; Villagomez-Castro, J.C.; Lopez-Romero, E.

Antonie van Leeuwenhoek

101

313-322

714553

21445884

Biochemical analysis and kinetic modeling of the thermal inactivation of MBP-fused heparinase I: implications for a comprehensive thermostabilization strategy

2011

Chen, S.; Ye, F.; Chen, Y.; Chen, Y.; Zhao, H.; Yatsunami, R.; Nakamura, S.; Arisaka, F.; Xing, X.H.

Biotechnol. Bioeng.

108

1841-1851

715029

19968989

Carbohydrate esterases of family 2 are 6-O-deacetylases

2010

Topakas, E.; Kyriakopoulos, S.; Biely, P.; Hirsch, J.; Vafiadi, C.; Christakopoulos, P.

FEBS Lett.

584

543-548

715128

20542873

A membrane-bound trehalase from Chironomus riparius larvae: purification and sensitivity to inhibition

2010

Forcella, M.; Cardona, F.; Goti, A.; Parmeggiani, C.; Cipolla, L.; Gregori, M.; Schirone, R.; Fusi, P.; Parenti, P.

Glycobiology

20

1186-1195

715178

20691783

The catalytic and other residues essential for the activity of the midgut trehalase from Spodoptera frugiperda

2010

Silva, M.C.; Terra, W.R.; Ferreira, C.

Insect Biochem. Mol. Biol.

40

733-741

715680

20870456

Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures

2011

Ujita, M.; Yamanaka, M.; Maeno, Y.; Yoshida, K.; Ohshio, W.; Ueno, Y.; Banno, Y.; Fujii, H.; Okumura, H.

J. Biosci. Bioeng.

111

22-25

716128

20630729

Characterization of Beauveria bassiana neutral trehalase (BbNTH1) and recognition of crucial stress-responsive elements to control its expression in response to multiple stresses

2011

Liu, Q.; Ying, S.H.; Feng, M.G.

Microbiol. Res.

166

282-293

720962

20713411

Rational engineering of Lactobacillus acidophilus NCFM maltose phosphorylase into either trehalose or kojibiose dual specificity phosphorylase

2010

Nakai, H.; Petersen, B.O.; Westphal, Y.; Dilokpimol, A.; Abou Hachem, M.; Duus, J.?.; Schols, H.A.; Svensson, B.

Protein Eng. Des. Sel.

23

781-787

721191

20383018

Crystallization and X-ray diffraction studies of inverting trehalose phosphorylase from Thermoanaerobacter sp.

2010

Van Hoorebeke, A.; Stout, J.; Van der Meeren, R.; Kyndt, J.; Van Beeumen, J.; Savvides, S.N.

Acta Crystallogr. Sect. F

66

442-447

721346

21803886

Enzymatic properties and substrate specificity of the trehalose phosphorylase from Caldanaerobacter subterraneus

2011

Van der Borght, J.; Chen, C.; Hoflack, L.; Van Renterghem, L.; Desmet, T.; Soetaert, W.

Appl. Environ. Microbiol.

77

6939-6944

721896

20799297

Enzymatic production of beta-D-glucose-1-phosphate from trehalose

2010

Van der Borght, J.; Desmet, T.; Soetaert, W.

Biotechnol. J.

5

986-993

723675

-

Catalytic reversibility of Pyrococcus horikoshii trehalose synthase: Efficient synthesis of several nucleoside diphosphate glucoses with enzyme recycling

2011

Ryu, S.; Kim, J.; Kim, E.; Chung, S.; Lee, S.

Process Biochem.

46

128-134

723932

15866533

Kinetic measurements and mechanism determination of Stf0 sulfotransferase using mass spectrometry

2005

Pi, N.; Hoang, M.B.; Gao, H.; Mougous, J.D.; Bertozzi, C.R.; Leary, J.A.

Anal. Biochem.

341

94-104

726029

15258569

Identification, function and structure of the mycobacterial sulfotransferase that initiates sulfolipid-1 biosynthesis

2004

Mougous, J.D.; Petzold, C.J.; Senaratne, R.H.; Lee, D.H.; Akey, D.L.; Lin, F.L.; Munchel, S.E.; Pratt, M.R.; Riley, L.W.; Leary, J.A.; Berger, J.M.; Bertozzi, C.R.

Nat. Struct. Mol. Biol.

11

721-729

727433

22402678

Effects of the N-terminal and C-terminal domains of Meiothermus ruber CBS-01 trehalose synthase on thermostability and activity

2012

Wang, Y.; Zhang, J.; Wang, W.; Liu, Y.; Xing, L.; Li, M.

Extremophiles

16

377-385

727887

21840994

Mechanistic analysis of trehalose synthase from Mycobacterium smegmatis

2011

Zhang, R.; Pan, Y.T.; He, S.; Lam, M.; Brayer, G.D.; Elbein, A.D.; Withers, S.G.

J. Biol. Chem.

286

35601-35609

729103

22484163

Purification and characterization of a trehalase-invertase enzyme with dual activity from Candida utilis

2012

Lahiri, S.; Basu, A.; Sengupta, S.; Banerjee, S.; Dutta, T.; Soren, D.; Chattopadhyay, K.; Ghosh, A.K.

Arch. Biochem. Biophys.

522

90-99

729110

22851503

Gene identification and enzymatic properties of a membrane-bound trehalase from the ovary of Rhodnius prolixus

2012

Santos, R.; Alves-Bezerra, M.; Rosas-Oliveira, R.; Majerowicz, D.; Meyer-Fernandes, J.R.; Gondim, K.C.

Arch. Insect Biochem. Physiol.

81

199-213

729533

17559818

Synthesis of mono- and dideoxygenated alpha,alpha-trehalose analogs

2007

Lin, F.L.; van Halbeek, H.; Bertozzi, C.R.

Carbohydr. Res.

342

2014-2030

729539

24680508

N-Bridged 1-deoxynojirimycin dimers as selective insect trehalase inhibitors

2014

Cipolla, L.; Sgambato, A.; Forcella, M.; Fusi, P.; Parenti, P.; Cardona, F.; Bini, D.

Carbohydr. Res.

389

46-49

729803

23613237

cDNA cloning and characterization of two trehalases from Spodoptera litura (Lepidoptera; Noctuidade)

2013

Zou, Q.; Wei, P.; Xu, Q.; Zheng, H.Z.; Tang, B.; Wang, S.G.

Genet. Mol. Res.

12

901-915

729830

23770497

Active site characterization and molecular cloning of Tenebrio molitor midgut trehalase and comments on their insect homologs

2013

Gomez, A.; Cardoso, C.; Genta, F.A.; Terra, W.R.; Ferreira, C.

Insect Biochem. Mol. Biol.

43

768-780

730096

25179874

Production of a periplasmic trehalase in Gluconobacter oxydans and growth on trehalose

2014

Kosciow, K.; Zahid, N.; Schweiger, P.; Deppenmeier, U.

J. Biotechnol.

189C

27-35

730189

25179903

Effects of temperature and additives on the thermal stability of glucoamylase from Aspergillus niger

2014

Liu, Y.; Meng, Z.; Shi, R.; Zhan, L.; Hu, W.; Xiang, H.; Xie, Q.

J. Microbiol. Biotechnol.

24

33-43

730653

23656873

Trehalases: a neglected carbon metabolism regulator?

2013

Barraza, A.; Sanchez, F.

Plant Signal. Behav.

8

e24778

730763

25003205

Trehalase regulates neuroepithelial stem cell maintenance and differentiation in the Drosophila optic lobe

2014

Chen, X.; Quan, Y.; Wang, H.; Luo, H.

PLoS ONE

9

e101433

730793

24992307

Structure of the trehalose-6-phosphate phosphatase from Brugia malayi reveals key design principles for anthelmintic drugs

2014

Farelli, J.D.; Galvin, B.D.; Li, Z.; Liu, C.; Aono, M.; Garland, M.; Hallett, O.E.; Causey, T.B.; Ali-Reynolds, A.; Saltzberg, D.J.; Carlow, C.K.; Dunaway-Mariano, D.; Allen, K.N.

PLoS Pathog.

10

e1004245

730924

23116628

Intracellular trehalase activity is required for development, germination and heat-stress resistance of Aspergillus niger conidia

2013

Svanstroem, A.; Melin, P.

Res. Microbiol.

164

91-99

731011

-

Purification and characterization of trehalase from seeds of chickpea (Cicer arietinum L.)

2013

Kord, M.; Youssef, E.; Ahmed, H.; Qaid, E.

Turk. J. Biol.

37

661-669

736088

24255995

Structural and mutational analysis of substrate recognition in kojibiose phosphorylase

2014

Okada, S.; Yamamoto, T.; Watanabe, H.; Nishimoto, T.; Chaen, H.; Fukuda, S.; Wakagi, T.; Fushinobu, S.

FEBS J.

281

778-786

736104

24021648

Potassium ion-dependent trehalose phosphorylase from halophilic Bacillus selenitireducens MLS10

2013

Nihira, T.; Saito, Y.; Chiku, K.; Kitaoka, M.; Ohtsubo, K.; Nakai, H.

FEBS Lett.

587

3382-3386

736332

2160944

Trehalose transport and metabolism in Escherichia coli

1990

Boos, W.; Ehmann, U.; Forkl, H.; Klein, W.; Rimmele, M.; Postma, P.

J. Bacteriol.

172

3450-3461

736338

7608078

Molecular analysis of treB encoding the Escherichia coli enzyme II specific for trehalose

1995

Klein, W.; Horlacher, R.; Boos, W.

J. Bacteriol.

177

4043-4052

736763

12949193

Glucose and trehalose PTS permeases of Spiroplasma citri probably share a single IIA domain, enabling the spiroplasma to adapt quickly to carbohydrate changes in its environment

2003

Andre, A.; Maccheroni, W.; Doignon, F.; Garnier, M.; Renaudin, J.

Microbiology

149

2687-2696

737143

24586369

The trehalose phosphotransferase system (PTS) in E. coli W can transport low levels of sucrose that are sufficient to facilitate induction of the csc sucrose catabolism operon

2014

Steen, J.A.; Bohlke, N.; Vickers, C.E.; Nielsen, L.K.

PLoS ONE

9

e88688

737237

-

Properties of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in third-stage larvae of the nematode Anisakis simplex - preliminary studies

2014

Lopienska-Biernat, E.; Czubak, M.; Zaobidna, E.; Rokicki, J.

Russ. J. Nematol.

22

131-140

739003

-

A cytosolic trehalase from the thermophilic fungus Humicola grisea var. thermoidea

1994

Cardello, L.; Terenzi, H. F.; Jorge, J. A.

Microbiology

140

1671-1677

739878

24585380

Purification and characterization of the glucoside 3-dehydrogenase produced by a newly isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251

2014

Zhang, J.F.; Yang, B.; Chen, W.; Chen, J.

Appl. Biochem. Biotechnol.

172

3913-3925

740778

25912449

Novel glucose dehydrogenase from Mucor prainii: Purification, characterization, molecular cloning and gene expression in Aspergillus sojae

2015

Satake, R.; Ichiyanagi, A.; Ichikawa, K.; Hirokawa, K.; Araki, Y.; Yoshimura, T.; Gomi, K.

J. Biosci. Bioeng.

120

498-503

740853

25022525

Efficient expression, purification, and characterization of a novel FAD-dependent glucose dehydrogenase from Aspergillus terreus in Pichia pastoris

2014

Yang, Y.; Huang, L.; Wang, J.; Wang, X.; Xu, Z.

J. Microbiol. Biotechnol.

24

1516-1524

741470

28044272

Gene cloning and expression of a glucoside 3-dehydrogenase from Sphingobacterium faecium ZJF-D6, and used it to produce N-p-nitrophenyl-3-ketovalidamine

2017

Zhang, J.F.; Chen, W.Q.; Chen, H.

World J. Microbiol. Biotechnol.

33

21

741947

8980633

Characterization of trehalase activities from the thermophilic fungus Scytalidium thermophilum

1996

Kadowaki, M.K.; Polizeli, M.L.; Terenzi, H.F.; Jorge, J.A.

Biochim. Biophys. Acta

1291

199-205

741949

15809023

Biochemical characterisation of the trehalase of thermophilic fungi an enzyme with mixed properties of neutral and acid trehalase

2005

Lucio-Eterovic, A.K.; Jorge, J.A.; Polizeli, M.d.e. .L.; Terenzi, H.F.

Biochim. Biophys. Acta

1723

201-207

747674

-

Molecular cloning and characterization of trehalose synthase from Thermotoga maritima DSM3109 Syntheses of trehalose disaccharide analogues and NDP-glucoses

2010

Ryu, S.; Kim, J.; Huong, N.; Woo, E.; Moon, S.; Lee, S.

Enzyme Microb. Technol.

47

249-256

747681

27871371

Effect of C-terminal domain truncation of Thermus thermophilus trehalose synthase on its substrate specificity

2017

Cho, C.B.; Park, D.Y.; Lee, S.B.

Enzyme Microb. Technol.

96

121-126

749959

27133444

Yeast trehalases Two enzymes, one catalytic mission

2016

Maicas, S.; Guirao-Abad, J.; Argüelles, J.

Biochim. Biophys. Acta

1860

2249-2254

750129

29411859

Split trehalase as a versatile reporter for a wide range of biological analytes

2018

Drikic, M.; De Buck, J.

Biotechnol. Bioeng.

115

1128-1136

750191

26822136

A trehalase from Zunongwangia sp. characterization and improving catalytic efficiency by directed evolution

2016

Cheng, Q.; Gao, H.; Hu, N.

BMC Biotechnol.

16

9

750527

30657252

Mechanistic insights into enzymatic catalysis by trehalase from the insect gut endosymbiont Enterobacter cloacae

2019

Adhav, A.; Harne, S.; Bhide, A.; Giri, A.; Gayathri, P.; Joshi, R.

FEBS J.

286

1700-1716

750693

29719214

First echinoderm trehalase from a tropical sea cucumber (Holothuria leucospilota) Molecular cloning and mRNA expression in different tissues, embryonic and larval stages, and under a starvation challenge

2018

Huo, D.; Jiang, X.; Wu, X.; Ren, C.; Yu, Z.; Liu, J.; Li, H.; Ruan, Y.; Wen, J.; Chen, T.; Hu, C.

Gene

665

74-81

750739

29844238

The periplasmic trehalase affects type 1 fimbria production and virulence of extraintestinal pathogenic Escherichia coli strain MT78

2018

Pavanelo, D.B.; Houle, S.; Matter, L.B.; Dozois, C.M.; Horn, F.

Infect. Immun.

86

e00241-18

750787

29412176

Functional characterization of Helicoverpa armigera trehalase and investigation of physiological effects caused due to its inhibition by validamycin A formulation

2018

Adhav, A.S.; Kokane, S.R.; Joshi, R.S.

Int. J. Biol. Macromol.

112

638-647

750882

30828477

Trehalase localization in the cerebral cortex, hippocampus and cerebellum of mouse brains

2019

Halbe, L.; Rami, A.

J. Adv. Res.

18

71-79

750956

25331437

The rv1184c locus encodes Chp2, an acyltransferase in Mycobacterium tuberculosis polyacyltrehalose lipid biosynthesis

2015

Touchette, M.H.; Holsclaw, C.M.; Previti, M.L.; Solomon, V.C.; Leary, J.A.; Bertozzi, C.R.; Seeliger, J.C.

J. Bacteriol.

197

201-210

751021

19276083

PapA3 is an acyltransferase required for polyacyltrehalose biosynthesis in Mycobacterium tuberculosis

2009

Hatzios, S.K.; Schelle, M.W.; Holsclaw, C.M.; Behrens, C.R.; Botyanszki, Z.; Lin, F.L.; Carlson, B.L.; Kumar, P.; Leary, J.A.; Bertozzi, C.R.

J. Biol. Chem.

284

12745-12751

751039

25124040

Biosynthesis and translocation of unsulfated acyltrehaloses in Mycobacterium tuberculosis

2014

Belardinelli, J.M.; Larrouy-Maumus, G.; Jones, V.; Sorio de Carvalho, L.P.; McNeil, M.R.; Jackson, M.

J. Biol. Chem.

289

27952-27965

751266

29912409

Gene cloning, prokaryotic expression, and biochemical characterization of a soluble trehalase in Helicoverpa armigera Huebner (Lepidoptera Noctuidae)

2018

Ai, D.; Cheng, S.; Chang, H.; Yang, T.; Wang, G.; Yu, C.

J. Insect Sci.

18

22

751313

29642287

Saci_1816 A Trehalase that catalyzes trehalose degradation in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius

2018

Lee, J.; Lee, A.; Moon, K.; Choi, K.H.; Cha, J.

J. Microbiol. Biotechnol.

28

909-916

754350

29912409

Gene cloning, prokaryotic expression, and biochemical characterization of a soluble trehalase in Helicoverpa armigera Huebner (Lepidoptera Noctuidae)

2018

Ai, D.; Cheng, S.; Chang, H.; Yang, T.; Wang, G.; Yu, C.

J. Insect Sci.

18

22

757361

-

Protective roles of trehalose in Pleurotus pulmonarius during heat stress response

2019

Liu, X.; Wu, X.; Gao, W.; Qu, J.; Chen, Q.; Huang, C.; Zhang, J.

J. Integr. Agric.

18

428-437

757619

27312340

Changes in trehalose content, enzyme activity and gene expression related to trehalose metabolism in Flammulina velutipes under heat shock

2016

Liu, J.H.; Shang, X.D.; Liu, J.Y.; Tan, Q.

Microbiology

162

1274-1285

758261

-

Enhancing the thermostability of recombinant cyclodextrin glucanotransferase via optimized stabilizer

2018

Zhang, J.; Li, M.; Zhang, Y.

Process Biochem.

67

64-70

759128

29859604

Combination of sequence-based and in silico screening to identify novel trehalose synthases

2018

Cai, X.; Seitl, I.; Mu, W.; Zhang, T.; Stressler, T.; Fischer, L.; Jiang, B.

Enzyme Microb. Technol.

115

62-72

759389

31290662

Computational and enzymatic analyses unveil the catalytic mechanism of thermostable trehalose synthase and suggest strategies for improved bioconversion

2019

Ren, X.; Wang, J.; Li, Y.; Wang, F.; Wang, R.; Li, P.; Ma, C.; Su, J.

J. Agric. Food Chem.

67

8177-8185

759527

31711928

Production and characterization of a recombinant thermophilic trehalose synthase from Thermus antranikianii

2020

Lin, Y.F.; Su, P.C.; Chen, P.T.

J. Biosci. Bioeng.

129

418-422

759622

-

Cloning, purification and characterization of a novel recombinant trehalose synthase (TreS) from Acidiplasma sp. MBA-1

2019

Al Faik, M.; Das, R.; Bo, J.; Mu, W.; Hassanin, H.

J. Microbiol. Biotechnol. Food Sci.

8

1298-1302

760081

27751933

Inducible expression of trehalose synthase in Bacillus licheniformis

2017

Li, Y.; Gu, Z.; Zhang, L.; Ding, Z.; Shi, G.

Protein Expr. Purif.

130

115-122

762577

29717994

A cryoprotectant induces conformational change in glyceraldehyde-3-phosphate dehydrogenase

2018

Kim, Y.J.

Acta Crystallogr. Sect. F

74

277-282